Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro

B. S.S. Rao, R. Shanbhoge, B. N. Rao, S. K. Adiga, D. Upadhya, B. K. Aithal, M. R.S. Kumar

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Abstract

This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 μg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 μg/mL CCE for 1h before 2Gy of radiation. Similarly, there was a significant (P<0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125μg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalHuman and Experimental Toxicology
Volume28
Issue number4
DOIs
Publication statusPublished - 04-2009

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Cymbopogon
Scavenging
DNA Damage
Free Radicals
Radiation
DNA
Dosimetry
Lipids
Micronucleus Tests
Sulfonic Acids
Gamma Rays
Fibroblasts
Cricetulus
Superoxides
Gamma rays
Hydroxyl Radical
Assays
Nitric Oxide
Antioxidants
Cells

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro",
abstract = "This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 μg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85{\%}), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77{\%}), hydroxyl (70{\%}), superoxide (76{\%}), nitric oxide (78{\%}) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57{\%}). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 μg/mL CCE for 1h before 2Gy of radiation. Similarly, there was a significant (P<0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125μg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.",
author = "Rao, {B. S.S.} and R. Shanbhoge and Rao, {B. N.} and Adiga, {S. K.} and D. Upadhya and Aithal, {B. K.} and Kumar, {M. R.S.}",
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AU - Rao, B. S.S.

AU - Shanbhoge, R.

AU - Rao, B. N.

AU - Adiga, S. K.

AU - Upadhya, D.

AU - Aithal, B. K.

AU - Kumar, M. R.S.

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N2 - This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 μg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 μg/mL CCE for 1h before 2Gy of radiation. Similarly, there was a significant (P<0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125μg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.

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