Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy

Surekha Barkur, Aseefhali Bankapur, Madhura Pradhan, Santhosh Chidangil, Deepak Mathur, Uma Ladiwala

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

Original languageEnglish
Article number085001
JournalJournal of Biomedical Optics
Volume20
Issue number8
DOIs
Publication statusPublished - 01-08-2015

Fingerprint

Stem cells
RNA
cultured cells
Stretching
Raman spectroscopy
Tumors
DNA
cancer
Cells
Proteins
cells
stem cells
tumors
deoxyribonucleic acid
proteins
augmentation

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

@article{9532cfc02d114eb4bdd51231b630a7c7,
title = "Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy",
abstract = "Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.",
author = "Surekha Barkur and Aseefhali Bankapur and Madhura Pradhan and Santhosh Chidangil and Deepak Mathur and Uma Ladiwala",
year = "2015",
month = "8",
day = "1",
doi = "10.1117/1.JBO.20.8.085001",
language = "English",
volume = "20",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "8",

}

Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy. / Barkur, Surekha; Bankapur, Aseefhali; Pradhan, Madhura; Chidangil, Santhosh; Mathur, Deepak; Ladiwala, Uma.

In: Journal of Biomedical Optics, Vol. 20, No. 8, 085001, 01.08.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy

AU - Barkur, Surekha

AU - Bankapur, Aseefhali

AU - Pradhan, Madhura

AU - Chidangil, Santhosh

AU - Mathur, Deepak

AU - Ladiwala, Uma

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

AB - Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

UR - http://www.scopus.com/inward/record.url?scp=84938916159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938916159&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.20.8.085001

DO - 10.1117/1.JBO.20.8.085001

M3 - Article

C2 - 26244913

AN - SCOPUS:84938916159

VL - 20

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 8

M1 - 085001

ER -