Abstract
The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.
| Original language | English |
|---|---|
| Article number | e201700394 |
| Journal | Journal of Biophotonics |
| Volume | 11 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 01-09-2018 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
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Probing endogenous collagen by laser-induced autofluorescence in burn wound biopsies : A pilot study. / Prabhu, Vijendra; Acharya, Anusha; Satish Rao, Bola S.; Rathnakar, Bharath; Kumar, Pramod; Guddattu, Vasudeva; Mahato, Krishna K.
In: Journal of Biophotonics, Vol. 11, No. 9, e201700394, 01.09.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - Probing endogenous collagen by laser-induced autofluorescence in burn wound biopsies
T2 - A pilot study
AU - Prabhu, Vijendra
AU - Acharya, Anusha
AU - Satish Rao, Bola S.
AU - Rathnakar, Bharath
AU - Kumar, Pramod
AU - Guddattu, Vasudeva
AU - Mahato, Krishna K.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.
AB - The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.
UR - http://www.scopus.com/inward/record.url?scp=85052829301&partnerID=8YFLogxK
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U2 - 10.1002/jbio.201700394
DO - 10.1002/jbio.201700394
M3 - Article
VL - 11
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 9
M1 - e201700394
ER -