Probing endogenous collagen by laser-induced autofluorescence in burn wound biopsies

A pilot study

Vijendra Prabhu, Anusha Acharya, Bola S. Satish Rao, Bharath Rathnakar, Pramod Kumar, Vasudeva Guddattu, Krishna K. Mahato

Research output: Contribution to journalArticle

Abstract

The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.

Original languageEnglish
Article numbere201700394
JournalJournal of Biophotonics
Volume11
Issue number9
DOIs
Publication statusPublished - 01-09-2018

Fingerprint

Biopsy
collagens
Collagen
Granulation Tissue
Lasers
Granulation
Tissue
Wounds and Injuries
Hydroxyproline
lasers
nicotinamide
normalizing
NAD
adenines
Tissue Donors
synthesis
excitation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

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title = "Probing endogenous collagen by laser-induced autofluorescence in burn wound biopsies: A pilot study",
abstract = "The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.",
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Probing endogenous collagen by laser-induced autofluorescence in burn wound biopsies : A pilot study. / Prabhu, Vijendra; Acharya, Anusha; Satish Rao, Bola S.; Rathnakar, Bharath; Kumar, Pramod; Guddattu, Vasudeva; Mahato, Krishna K.

In: Journal of Biophotonics, Vol. 11, No. 9, e201700394, 01.09.2018.

Research output: Contribution to journalArticle

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AU - Prabhu, Vijendra

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AU - Satish Rao, Bola S.

AU - Rathnakar, Bharath

AU - Kumar, Pramod

AU - Guddattu, Vasudeva

AU - Mahato, Krishna K.

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