Probing oxidative stress in single erythrocytes with Raman Tweezers

E. Zachariah, A. Bankapur, C. Santhosh, M. Valiathan, D. Mathur

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to S. S and C-S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.

Original languageEnglish
Pages (from-to)113-116
Number of pages4
JournalJournal of Photochemistry and Photobiology B: Biology
Volume100
Issue number3
DOIs
Publication statusPublished - 01-09-2010

Fingerprint

erythrocytes
Oxidative Stress
Erythrocytes
glutathione
Glutathione
Principal Component Analysis
Sulfhydryl Compounds
Disulfides
augmentation
disulfides
principal components analysis
thiols
discrimination
damage
proteins
Proteins
cells

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

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Probing oxidative stress in single erythrocytes with Raman Tweezers. / Zachariah, E.; Bankapur, A.; Santhosh, C.; Valiathan, M.; Mathur, D.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 100, No. 3, 01.09.2010, p. 113-116.

Research output: Contribution to journalArticle

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AU - Santhosh, C.

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AU - Mathur, D.

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