Process optimization for tannase production by Bacillus gottheilii M2S2 on inert polyurethane foam support

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tannin acyl hydrolase (E.C. 3.1.1.20), commonly referred as "tannase", breaks hydrolysable tannins into gallic acid and glucose. It finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. In this work, a tannase producing Bacillus gottheilii M2S2 was isolated from the tannery effluent soil and a sequential optimization strategy was used to determine the effect of carbon source and inducer-tannic acid, nitrogen source NH4NO3, inoculum volume, initial pH, incubation time and mineral salts (KH2PO4, MgSO4, NaCl and CaCl2�2H2O) in the production of tannase from Bacillus gottheilii M2S2 in solid state fermentation. Polyurethane foam was used as an inert support for growth of the microorganism. The microorganism was fermented at 32 �C in an optimized liquid medium composed of tannic acid (4%), NH4NO3 (2%), KH2PO4 (0.1%), MgSO4 (0.2%), NaCl (0.1%) and CaCl2�2H2O (0.05%) adjusted to initial media pH 4.74 then inoculated with 7.035% vv-1 inoculum and impregnated on polyurethane foam for 26.45 h under static condition A final activity of 49.32�0.30 U L-1 was achieved, which represents an increase of 328% in relation to the initial unoptimized conditions.

Original languageEnglish
Pages (from-to)48-55
Number of pages8
JournalBiocatalysis and Agricultural Biotechnology
Volume7
DOIs
Publication statusPublished - 01-07-2016

Fingerprint

tannase
polyurethanes
Tannins
Bacilli
foams
Bacillus
Polyurethanes
tannins
Foams
Gallic Acid
Acids
Microorganisms
ammonium nitrate
gallic acid
Fruit juices
Hydrolyzable Tannins
inoculum
Hydrolases
tannery effluents
Food Industry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

@article{51caa098f6054447b2e19820461860f5,
title = "Process optimization for tannase production by Bacillus gottheilii M2S2 on inert polyurethane foam support",
abstract = "Tannin acyl hydrolase (E.C. 3.1.1.20), commonly referred as {"}tannase{"}, breaks hydrolysable tannins into gallic acid and glucose. It finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. In this work, a tannase producing Bacillus gottheilii M2S2 was isolated from the tannery effluent soil and a sequential optimization strategy was used to determine the effect of carbon source and inducer-tannic acid, nitrogen source NH4NO3, inoculum volume, initial pH, incubation time and mineral salts (KH2PO4, MgSO4, NaCl and CaCl2{\"i}¿½2H2O) in the production of tannase from Bacillus gottheilii M2S2 in solid state fermentation. Polyurethane foam was used as an inert support for growth of the microorganism. The microorganism was fermented at 32 {\"i}¿½C in an optimized liquid medium composed of tannic acid (4{\%}), NH4NO3 (2{\%}), KH2PO4 (0.1{\%}), MgSO4 (0.2{\%}), NaCl (0.1{\%}) and CaCl2{\"i}¿½2H2O (0.05{\%}) adjusted to initial media pH 4.74 then inoculated with 7.035{\%} vv-1 inoculum and impregnated on polyurethane foam for 26.45 h under static condition A final activity of 49.32{\"i}¿½0.30 U L-1 was achieved, which represents an increase of 328{\%} in relation to the initial unoptimized conditions.",
author = "S. Subbalaxmi and Murty, {Vytla Ramachandra}",
year = "2016",
month = "7",
day = "1",
doi = "10.1016/j.bcab.2016.05.004",
language = "English",
volume = "7",
pages = "48--55",
journal = "Biocatalysis and Agricultural Biotechnology",
issn = "1878-8181",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Process optimization for tannase production by Bacillus gottheilii M2S2 on inert polyurethane foam support

AU - Subbalaxmi, S.

AU - Murty, Vytla Ramachandra

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Tannin acyl hydrolase (E.C. 3.1.1.20), commonly referred as "tannase", breaks hydrolysable tannins into gallic acid and glucose. It finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. In this work, a tannase producing Bacillus gottheilii M2S2 was isolated from the tannery effluent soil and a sequential optimization strategy was used to determine the effect of carbon source and inducer-tannic acid, nitrogen source NH4NO3, inoculum volume, initial pH, incubation time and mineral salts (KH2PO4, MgSO4, NaCl and CaCl2�2H2O) in the production of tannase from Bacillus gottheilii M2S2 in solid state fermentation. Polyurethane foam was used as an inert support for growth of the microorganism. The microorganism was fermented at 32 �C in an optimized liquid medium composed of tannic acid (4%), NH4NO3 (2%), KH2PO4 (0.1%), MgSO4 (0.2%), NaCl (0.1%) and CaCl2�2H2O (0.05%) adjusted to initial media pH 4.74 then inoculated with 7.035% vv-1 inoculum and impregnated on polyurethane foam for 26.45 h under static condition A final activity of 49.32�0.30 U L-1 was achieved, which represents an increase of 328% in relation to the initial unoptimized conditions.

AB - Tannin acyl hydrolase (E.C. 3.1.1.20), commonly referred as "tannase", breaks hydrolysable tannins into gallic acid and glucose. It finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. In this work, a tannase producing Bacillus gottheilii M2S2 was isolated from the tannery effluent soil and a sequential optimization strategy was used to determine the effect of carbon source and inducer-tannic acid, nitrogen source NH4NO3, inoculum volume, initial pH, incubation time and mineral salts (KH2PO4, MgSO4, NaCl and CaCl2�2H2O) in the production of tannase from Bacillus gottheilii M2S2 in solid state fermentation. Polyurethane foam was used as an inert support for growth of the microorganism. The microorganism was fermented at 32 �C in an optimized liquid medium composed of tannic acid (4%), NH4NO3 (2%), KH2PO4 (0.1%), MgSO4 (0.2%), NaCl (0.1%) and CaCl2�2H2O (0.05%) adjusted to initial media pH 4.74 then inoculated with 7.035% vv-1 inoculum and impregnated on polyurethane foam for 26.45 h under static condition A final activity of 49.32�0.30 U L-1 was achieved, which represents an increase of 328% in relation to the initial unoptimized conditions.

UR - http://www.scopus.com/inward/record.url?scp=84968875316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84968875316&partnerID=8YFLogxK

U2 - 10.1016/j.bcab.2016.05.004

DO - 10.1016/j.bcab.2016.05.004

M3 - Article

VL - 7

SP - 48

EP - 55

JO - Biocatalysis and Agricultural Biotechnology

JF - Biocatalysis and Agricultural Biotechnology

SN - 1878-8181

ER -