Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds

An ex vivo study

Vijendra Prabhu, Anusha Acharya, Satish B.S. Rao, Pramod Kumar, Anurag Sharan, Krishna K. Mahato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Thermal injuries are considered as the most frequent and dreadful forms of trauma. Quick and objective assessment of treatment regimen plays vital role in controlling morbidity and mortality. In the present work, we aimed to investigate the predicative potential of laser induced fluorescence (LIF) in assessing collagen synthesis during the wound healing progression following thermal injury. To this end, granulation tissues of thermal wounds before grafting were collected from the patients of age group 18- 60 years with prior consent. LIF measurements of the wound granulation tissues were carried out by exciting them with 325 nm He-Cd laser (2 mW power). Collagen intensity with respect to normalized NADH levels in each of the samples was computed and tabulated. In addition, biochemical estimation of the hydroxyproline was also carried out from the same granulation tissues. Spectroscopic assessment of the wound granulation tissues displayed varied collagen levels indicating different degree of healing. Likewise, biochemical estimation of granulation tissues exhibited a wide range of hydroxyproline levels. The findings of the present ex vivo study suggest that both the techniques are equally informative in evaluating collagen content in tissues. However, with LIF, it is more advantageous in the sense that it is fast, objective, cost effective as well as non-destructive method. Thus, it can be concluded that laser induced fluorescence has the potential to be used as an objective tool to qualitatively determine the collagen levels in wound granulation tissues and provide information helpful for making clinical decisions.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics X
PublisherSPIE
Volume8926
ISBN (Print)9780819498397
DOIs
Publication statusPublished - 2014
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: 07-02-201508-02-2015

Conference

ConferencePhotonic Therapeutics and Diagnostics XI
CountryUnited States
CitySan Francisco
Period07-02-1508-02-15

Fingerprint

collagens
Granulation
Granulation Tissue
Collagen
laser induced fluorescence
Lasers
Hot Temperature
Fluorescence
Tissue
Wounds and Injuries
Hydroxyproline
wound healing
mortality
healing
progressions
NAD
Wound Healing
Age Groups
Morbidity
costs

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: An ex vivo study",
abstract = "Thermal injuries are considered as the most frequent and dreadful forms of trauma. Quick and objective assessment of treatment regimen plays vital role in controlling morbidity and mortality. In the present work, we aimed to investigate the predicative potential of laser induced fluorescence (LIF) in assessing collagen synthesis during the wound healing progression following thermal injury. To this end, granulation tissues of thermal wounds before grafting were collected from the patients of age group 18- 60 years with prior consent. LIF measurements of the wound granulation tissues were carried out by exciting them with 325 nm He-Cd laser (2 mW power). Collagen intensity with respect to normalized NADH levels in each of the samples was computed and tabulated. In addition, biochemical estimation of the hydroxyproline was also carried out from the same granulation tissues. Spectroscopic assessment of the wound granulation tissues displayed varied collagen levels indicating different degree of healing. Likewise, biochemical estimation of granulation tissues exhibited a wide range of hydroxyproline levels. The findings of the present ex vivo study suggest that both the techniques are equally informative in evaluating collagen content in tissues. However, with LIF, it is more advantageous in the sense that it is fast, objective, cost effective as well as non-destructive method. Thus, it can be concluded that laser induced fluorescence has the potential to be used as an objective tool to qualitatively determine the collagen levels in wound granulation tissues and provide information helpful for making clinical decisions.",
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Prabhu, V, Acharya, A, Rao, SBS, Kumar, P, Sharan, A & Mahato, KK 2014, Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: An ex vivo study. in Photonic Therapeutics and Diagnostics X. vol. 8926, 892608, SPIE, Photonic Therapeutics and Diagnostics XI, San Francisco, United States, 07-02-15. https://doi.org/10.1117/12.2039416

Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds : An ex vivo study. / Prabhu, Vijendra; Acharya, Anusha; Rao, Satish B.S.; Kumar, Pramod; Sharan, Anurag; Mahato, Krishna K.

Photonic Therapeutics and Diagnostics X. Vol. 8926 SPIE, 2014. 892608.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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