Promising anticancer activities of Justicia simplex D. Don. in cellular and animal models

Litty Joseph, Jesil Mathew Aranjani, K. Sreedhara Ranganath Pai, K. K. Srinivasan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ethnopharmacological relevance Justicia simplex D. Don. belonging to the family of Acanthaceae has been traditionally used for treatment of rheumatism, inflammation and bronchitis. The plant is traditionally considered as an anticancer medicine and is used by healers of Karnataka to treat various types of cancers. Aim of the study The present study aims at the elucidation of anticancer activity of various extracts of J. simplex, isolation of its active constituents and assessment of the role in growth inhibition and angiogenesis both in vitro and in vivo. Materials and methods Extracts of J. simplex was evaluated for the in vitro cytotoxic effect by Brine Shrimp Lethality assay, Trypan Blue dye exclusion assay and antiproliferative assay. In vivo cytotoxicity of the extracts were determined by liquid tumor model in Swiss albino mice. Tumor prognosis, metastasis and angiogenesis were assessed by VEGF expression of the solid tumor. Phytochemical analysis afforded the isolation of a compound, the chemical structure of which was established using IR, NMR and TOF-MS spectral method. The compound was also evaluated for the growth inhibitory and angiogenic effects. Results and conclusion The petroleum ether extract revealed potent anticancer activity in in vitro and in vivo studies. The anti-angiogenic effect is due to the down regulation of VEGF expression. The growth inhibitory assay revealed that the isolated compound namely triacontanoic ester of 5ʹʹ-hydroxyjustisolin is responsible for the anticancer activity.

Original languageEnglish
Pages (from-to)231-239
Number of pages9
JournalJournal of Ethnopharmacology
Volume199
DOIs
Publication statusPublished - 06-03-2017

Fingerprint

Justicia
Animal Models
Vascular Endothelial Growth Factor A
Neoplasms
Growth
Acanthaceae
Artemia
Trypan Blue
Bronchitis
Phytochemicals
Rheumatic Diseases
Esters
Coloring Agents
Down-Regulation
Medicine
Neoplasm Metastasis
Inflammation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

@article{b8a1824ae1a04b1b9e62839829f30362,
title = "Promising anticancer activities of Justicia simplex D. Don. in cellular and animal models",
abstract = "Ethnopharmacological relevance Justicia simplex D. Don. belonging to the family of Acanthaceae has been traditionally used for treatment of rheumatism, inflammation and bronchitis. The plant is traditionally considered as an anticancer medicine and is used by healers of Karnataka to treat various types of cancers. Aim of the study The present study aims at the elucidation of anticancer activity of various extracts of J. simplex, isolation of its active constituents and assessment of the role in growth inhibition and angiogenesis both in vitro and in vivo. Materials and methods Extracts of J. simplex was evaluated for the in vitro cytotoxic effect by Brine Shrimp Lethality assay, Trypan Blue dye exclusion assay and antiproliferative assay. In vivo cytotoxicity of the extracts were determined by liquid tumor model in Swiss albino mice. Tumor prognosis, metastasis and angiogenesis were assessed by VEGF expression of the solid tumor. Phytochemical analysis afforded the isolation of a compound, the chemical structure of which was established using IR, NMR and TOF-MS spectral method. The compound was also evaluated for the growth inhibitory and angiogenic effects. Results and conclusion The petroleum ether extract revealed potent anticancer activity in in vitro and in vivo studies. The anti-angiogenic effect is due to the down regulation of VEGF expression. The growth inhibitory assay revealed that the isolated compound namely triacontanoic ester of 5ʹʹ-hydroxyjustisolin is responsible for the anticancer activity.",
author = "Litty Joseph and Aranjani, {Jesil Mathew} and Pai, {K. Sreedhara Ranganath} and Srinivasan, {K. K.}",
year = "2017",
month = "3",
day = "6",
doi = "10.1016/j.jep.2017.01.046",
language = "English",
volume = "199",
pages = "231--239",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",

}

Promising anticancer activities of Justicia simplex D. Don. in cellular and animal models. / Joseph, Litty; Aranjani, Jesil Mathew; Pai, K. Sreedhara Ranganath; Srinivasan, K. K.

In: Journal of Ethnopharmacology, Vol. 199, 06.03.2017, p. 231-239.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Promising anticancer activities of Justicia simplex D. Don. in cellular and animal models

AU - Joseph, Litty

AU - Aranjani, Jesil Mathew

AU - Pai, K. Sreedhara Ranganath

AU - Srinivasan, K. K.

PY - 2017/3/6

Y1 - 2017/3/6

N2 - Ethnopharmacological relevance Justicia simplex D. Don. belonging to the family of Acanthaceae has been traditionally used for treatment of rheumatism, inflammation and bronchitis. The plant is traditionally considered as an anticancer medicine and is used by healers of Karnataka to treat various types of cancers. Aim of the study The present study aims at the elucidation of anticancer activity of various extracts of J. simplex, isolation of its active constituents and assessment of the role in growth inhibition and angiogenesis both in vitro and in vivo. Materials and methods Extracts of J. simplex was evaluated for the in vitro cytotoxic effect by Brine Shrimp Lethality assay, Trypan Blue dye exclusion assay and antiproliferative assay. In vivo cytotoxicity of the extracts were determined by liquid tumor model in Swiss albino mice. Tumor prognosis, metastasis and angiogenesis were assessed by VEGF expression of the solid tumor. Phytochemical analysis afforded the isolation of a compound, the chemical structure of which was established using IR, NMR and TOF-MS spectral method. The compound was also evaluated for the growth inhibitory and angiogenic effects. Results and conclusion The petroleum ether extract revealed potent anticancer activity in in vitro and in vivo studies. The anti-angiogenic effect is due to the down regulation of VEGF expression. The growth inhibitory assay revealed that the isolated compound namely triacontanoic ester of 5ʹʹ-hydroxyjustisolin is responsible for the anticancer activity.

AB - Ethnopharmacological relevance Justicia simplex D. Don. belonging to the family of Acanthaceae has been traditionally used for treatment of rheumatism, inflammation and bronchitis. The plant is traditionally considered as an anticancer medicine and is used by healers of Karnataka to treat various types of cancers. Aim of the study The present study aims at the elucidation of anticancer activity of various extracts of J. simplex, isolation of its active constituents and assessment of the role in growth inhibition and angiogenesis both in vitro and in vivo. Materials and methods Extracts of J. simplex was evaluated for the in vitro cytotoxic effect by Brine Shrimp Lethality assay, Trypan Blue dye exclusion assay and antiproliferative assay. In vivo cytotoxicity of the extracts were determined by liquid tumor model in Swiss albino mice. Tumor prognosis, metastasis and angiogenesis were assessed by VEGF expression of the solid tumor. Phytochemical analysis afforded the isolation of a compound, the chemical structure of which was established using IR, NMR and TOF-MS spectral method. The compound was also evaluated for the growth inhibitory and angiogenic effects. Results and conclusion The petroleum ether extract revealed potent anticancer activity in in vitro and in vivo studies. The anti-angiogenic effect is due to the down regulation of VEGF expression. The growth inhibitory assay revealed that the isolated compound namely triacontanoic ester of 5ʹʹ-hydroxyjustisolin is responsible for the anticancer activity.

UR - http://www.scopus.com/inward/record.url?scp=85012992162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85012992162&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2017.01.046

DO - 10.1016/j.jep.2017.01.046

M3 - Article

VL - 199

SP - 231

EP - 239

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

ER -