Protective effect by aqueous extract of Phyllanthus amarus Linn., phyllanthin and nirocil against carbontetrachloride-induced liver and brain toxicity

P. Venkatesan, K.S. Satyan, M. Sudheer Kumar, A. Prakash

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Effect of carbontetrachloride treatment on hepatic and brain antioxidant status in rats pretreated with aqueous extract of Phyllanthus amarus Linn. (Euphorbiaceae), nirocil (a tablet made up of aqueous extract of P. amarus), phyllanthin (a bioactive lignan from P. amarus), and silymarin were studied. Plasma aspartate aminotransferase and alanine aminotransferase were estimated to monitor the extent of hepatocellur damage. Tissue lipid peroxide, ascorbic acid and total protein levels were used as the markers for functional and antioxidant efficiency of liver and brain cells. Phyllanthin reversed the elevated plasma aminotransferase levels but did not affect hepatic antioxidant status. In all the paradigms tested for hepatoprotection, nirocil, Silymarin and aqueous extract (90 mg/kg) showed significant protection. There was a drastic impairment in the functional and antioxidant status of brain on treatment with carbontetrachloride. None of the drugs except silymarin showed good protection against carbontetrachloride-induced lipid peroxidation in the brain, but all these produced a significant increase in the protein levels. All the drugs administered, augmented the ascorbate levels in liver and brain, with the aqueous extract of P. amarus clearly outdoing the others.
Original languageEnglish
Pages (from-to)309-312
Number of pages4
JournalIndian Journal of Pharmaceutical Sciences
Volume65
Issue number3
Publication statusPublished - 2003

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Phyllanthus
Silymarin
Antioxidants
Liver
Brain
Euphorbiaceae
Lignans
Lipid Peroxides
Aspartate Aminotransferases
Transaminases
Alanine Transaminase
Pharmaceutical Preparations
Lipid Peroxidation
Tablets
Ascorbic Acid
Proteins
phyllanthin

Cite this

@article{1a2cfbea2c6c431ead32cf675bed2127,
title = "Protective effect by aqueous extract of Phyllanthus amarus Linn., phyllanthin and nirocil against carbontetrachloride-induced liver and brain toxicity",
abstract = "Effect of carbontetrachloride treatment on hepatic and brain antioxidant status in rats pretreated with aqueous extract of Phyllanthus amarus Linn. (Euphorbiaceae), nirocil (a tablet made up of aqueous extract of P. amarus), phyllanthin (a bioactive lignan from P. amarus), and silymarin were studied. Plasma aspartate aminotransferase and alanine aminotransferase were estimated to monitor the extent of hepatocellur damage. Tissue lipid peroxide, ascorbic acid and total protein levels were used as the markers for functional and antioxidant efficiency of liver and brain cells. Phyllanthin reversed the elevated plasma aminotransferase levels but did not affect hepatic antioxidant status. In all the paradigms tested for hepatoprotection, nirocil, Silymarin and aqueous extract (90 mg/kg) showed significant protection. There was a drastic impairment in the functional and antioxidant status of brain on treatment with carbontetrachloride. None of the drugs except silymarin showed good protection against carbontetrachloride-induced lipid peroxidation in the brain, but all these produced a significant increase in the protein levels. All the drugs administered, augmented the ascorbate levels in liver and brain, with the aqueous extract of P. amarus clearly outdoing the others.",
author = "P. Venkatesan and K.S. Satyan and {Sudheer Kumar}, M. and A. Prakash",
note = "Cited By :7 Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Venkatesan, P.; Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Manipal-576 119, India; email: badram@rediffmail.com References: Syamasundar, K.V., Singh, B., Thakur, R.S., Husain, A., Kiso, Y., Hino, H., (1985) J. Ethnopharmacol, 14, p. 41; Prakash, A., Satyan, K.S., Wahi, S.P., Singh, R.P., (1995) Phytother. Res, 9, p. 594; Olafsson, S., Gottstein, J., Blei, A.T., (1995) Gastroenterology, 108, p. 1097; Murry, R.K., (1993) Harper's Biochemistry, p. 829. , Murry, R.K, Garnner, D.K, Mayes, P.A. and Rodwell V.W, Eds, 25th Edn, Appleton & Lange, Conneticut, USA; Krishnamurthy, G.V., Seshadri, T.R., (1946) Proc. Indian Acad. Sci, 23, p. 357; Henry, R.J., Chiamori, N., Golub, O.J., Berkman, S., (1969) Amer. J. Clin. Path, 34, p. 381; Ohkawa, H., Ohishi, N., Yagi, K., (1979) Anal. Biochem, 95, p. 351; Roe, J.H., (1954) Methods in Biochemical Analysis, 1, p. 115. , Glick, D Eds, Inter Science Publishers, New York, USA, 1; Lowry, O.H., Rosebrough, N.J., Farr, A.I., Randall, R.J., (1951) J. Biol. Chem, 193, p. 265; Grunewald, R.A., (1993) Brain Res. Rev, 18, p. 123; Hillered, L., Nilsson, P., Ungerstedt, U., Ponter, U., (1990) Neurosci. Lett, 113, p. 328; Valenzuela, A., Lagos, C., Schimdt, K., Videla, I.A., (1985) Biochem. Pharmacol, 34, p. 2209; Miguez, M.P., Anundi, I., Sainz-Pardo, L.A., Lindros, K.O., (1994) Toxic. in vitro, 8, p. 581; Galvez, J., Jose Pedro de la Cruz. and Zarzuelo, A (1995) Pharmacology, 51, p. 127; Ubeda, A., Esteve, M.L., Alcaraz, M.J., Cheeseman, K.H., Slater, T.F., (1995) Phytother. Res, 9, p. 416; Balasubramanyam, K.A., Ramachandran, A., Thomas, S., Prabhu, R., (2001) International Conference on Natural Antioxidants and Free Radicals in Human Health and Radiation Biology, BARC, p. 22. , Mumbai, India; Sosnovsky, A.S., Salieva, R.A., Koplick, E.V., Kozlov, A.V., (1995) Ann. Natl. Acad. Med. Sci, 31, p. 115",
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Protective effect by aqueous extract of Phyllanthus amarus Linn., phyllanthin and nirocil against carbontetrachloride-induced liver and brain toxicity. / Venkatesan, P.; Satyan, K.S.; Sudheer Kumar, M.; Prakash, A.

In: Indian Journal of Pharmaceutical Sciences, Vol. 65, No. 3, 2003, p. 309-312.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect by aqueous extract of Phyllanthus amarus Linn., phyllanthin and nirocil against carbontetrachloride-induced liver and brain toxicity

AU - Venkatesan, P.

AU - Satyan, K.S.

AU - Sudheer Kumar, M.

AU - Prakash, A.

N1 - Cited By :7 Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Venkatesan, P.; Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Manipal-576 119, India; email: badram@rediffmail.com References: Syamasundar, K.V., Singh, B., Thakur, R.S., Husain, A., Kiso, Y., Hino, H., (1985) J. Ethnopharmacol, 14, p. 41; Prakash, A., Satyan, K.S., Wahi, S.P., Singh, R.P., (1995) Phytother. Res, 9, p. 594; Olafsson, S., Gottstein, J., Blei, A.T., (1995) Gastroenterology, 108, p. 1097; Murry, R.K., (1993) Harper's Biochemistry, p. 829. , Murry, R.K, Garnner, D.K, Mayes, P.A. and Rodwell V.W, Eds, 25th Edn, Appleton & Lange, Conneticut, USA; Krishnamurthy, G.V., Seshadri, T.R., (1946) Proc. Indian Acad. Sci, 23, p. 357; Henry, R.J., Chiamori, N., Golub, O.J., Berkman, S., (1969) Amer. J. Clin. Path, 34, p. 381; Ohkawa, H., Ohishi, N., Yagi, K., (1979) Anal. Biochem, 95, p. 351; Roe, J.H., (1954) Methods in Biochemical Analysis, 1, p. 115. , Glick, D Eds, Inter Science Publishers, New York, USA, 1; Lowry, O.H., Rosebrough, N.J., Farr, A.I., Randall, R.J., (1951) J. Biol. Chem, 193, p. 265; Grunewald, R.A., (1993) Brain Res. Rev, 18, p. 123; Hillered, L., Nilsson, P., Ungerstedt, U., Ponter, U., (1990) Neurosci. Lett, 113, p. 328; Valenzuela, A., Lagos, C., Schimdt, K., Videla, I.A., (1985) Biochem. Pharmacol, 34, p. 2209; Miguez, M.P., Anundi, I., Sainz-Pardo, L.A., Lindros, K.O., (1994) Toxic. in vitro, 8, p. 581; Galvez, J., Jose Pedro de la Cruz. and Zarzuelo, A (1995) Pharmacology, 51, p. 127; Ubeda, A., Esteve, M.L., Alcaraz, M.J., Cheeseman, K.H., Slater, T.F., (1995) Phytother. Res, 9, p. 416; Balasubramanyam, K.A., Ramachandran, A., Thomas, S., Prabhu, R., (2001) International Conference on Natural Antioxidants and Free Radicals in Human Health and Radiation Biology, BARC, p. 22. , Mumbai, India; Sosnovsky, A.S., Salieva, R.A., Koplick, E.V., Kozlov, A.V., (1995) Ann. Natl. Acad. Med. Sci, 31, p. 115

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Y1 - 2003

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AB - Effect of carbontetrachloride treatment on hepatic and brain antioxidant status in rats pretreated with aqueous extract of Phyllanthus amarus Linn. (Euphorbiaceae), nirocil (a tablet made up of aqueous extract of P. amarus), phyllanthin (a bioactive lignan from P. amarus), and silymarin were studied. Plasma aspartate aminotransferase and alanine aminotransferase were estimated to monitor the extent of hepatocellur damage. Tissue lipid peroxide, ascorbic acid and total protein levels were used as the markers for functional and antioxidant efficiency of liver and brain cells. Phyllanthin reversed the elevated plasma aminotransferase levels but did not affect hepatic antioxidant status. In all the paradigms tested for hepatoprotection, nirocil, Silymarin and aqueous extract (90 mg/kg) showed significant protection. There was a drastic impairment in the functional and antioxidant status of brain on treatment with carbontetrachloride. None of the drugs except silymarin showed good protection against carbontetrachloride-induced lipid peroxidation in the brain, but all these produced a significant increase in the protein levels. All the drugs administered, augmented the ascorbate levels in liver and brain, with the aqueous extract of P. amarus clearly outdoing the others.

M3 - Article

VL - 65

SP - 309

EP - 312

JO - Indian Journal of Pharmaceutical Sciences

JF - Indian Journal of Pharmaceutical Sciences

SN - 0250-474X

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ER -