Protective effect of Azadirachta indica A. Juss against doxorubicin-induced cardiac toxicity in tumour bearing mice

Ashwani Koul, Renu Goyal, Sanjay Bharati

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Doxorubicin (DOX) treatment (12 μg/g body weight, once a week for 2 weeks) resulted in a significant decrease in the heart rate along with an increase in QRS, ST, and QT intervals. Histopathological studies showed cardiomyocyte degeneration, cytoplasmic vacuolation and macrophage infiltration in cardiac tissue. A marked increase in the rate of apoptosis was also observed. An increased oxidative stress was evidenced by significantly higher levels of lipid peroxidation (LPO) and depletion of reduced glutathione. A decrease in the activity of cellular antioxidant defence enzymes was also observed. The decrease in the heart rate and ECG alterations were prevented significantly by AAILE (100 μg/g body weight, po) co-treatment, started two weeks prior to DOX treatment and continued till the termination of the experiment. The cardioprotection was also evident from histopathology and decrease in the rate of apoptosis in cardiomyocytes. AAILE co-treatment also prevented DOX-induced increase in LPO and decrease in antioxidant defence enzymes. The results suggest that AAILE administration prevents DOX-induced cardiotoxicity.

Original languageEnglish
Pages (from-to)323-331
Number of pages9
JournalIndian Journal of Experimental Biology
Volume52
Issue number4
Publication statusPublished - 01-01-2014

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Azadirachta
Doxorubicin
Cardiac Myocytes
Lipid Peroxidation
Neoplasms
Antioxidants
Heart Rate
Body Weight
Apoptosis
Enzymes
Glutathione
Electrocardiography
Oxidative Stress
Macrophages
Cardiotoxicity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Cell Biology
  • Molecular Biology

Cite this

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Protective effect of Azadirachta indica A. Juss against doxorubicin-induced cardiac toxicity in tumour bearing mice. / Koul, Ashwani; Goyal, Renu; Bharati, Sanjay.

In: Indian Journal of Experimental Biology, Vol. 52, No. 4, 01.01.2014, p. 323-331.

Research output: Contribution to journalArticle

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AB - Doxorubicin (DOX) treatment (12 μg/g body weight, once a week for 2 weeks) resulted in a significant decrease in the heart rate along with an increase in QRS, ST, and QT intervals. Histopathological studies showed cardiomyocyte degeneration, cytoplasmic vacuolation and macrophage infiltration in cardiac tissue. A marked increase in the rate of apoptosis was also observed. An increased oxidative stress was evidenced by significantly higher levels of lipid peroxidation (LPO) and depletion of reduced glutathione. A decrease in the activity of cellular antioxidant defence enzymes was also observed. The decrease in the heart rate and ECG alterations were prevented significantly by AAILE (100 μg/g body weight, po) co-treatment, started two weeks prior to DOX treatment and continued till the termination of the experiment. The cardioprotection was also evident from histopathology and decrease in the rate of apoptosis in cardiomyocytes. AAILE co-treatment also prevented DOX-induced increase in LPO and decrease in antioxidant defence enzymes. The results suggest that AAILE administration prevents DOX-induced cardiotoxicity.

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