Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats

A. Shirwaikar, S. Malini, S.C. Kumari

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Ethanolic extract of flowers of Pongamia pinnata was studied for its protective effect against cisplatin and gentamicin induced renal injury in rats. When the extract (300 & 600 mg kg-1) was administered orally for 10 days following cisplatin (5 mg kg-1 ip) on day 5, toxicity of cisplatin, as measured by loss of body weight, elevated blood urea and serum creatinine declined significantly. Similarly in gentamicin (40 mg kg-1 s.c) induced renal injury, the extract (600 mg kg-1) normalized the raised blood urea and serum creatinine levels. Reversal of cisplatin and gentamicin renal cell damage as induced by tubular necrosis ie, marked congestion of the glomeruli with glomerular atrophy, degeneration of tubular epithelial cells with casts in the tubular lumen and infiltration of inflammatory cells in the interstitium was confirmed on histopathological examination. In the preventive regimen, co-administration of the extract with gentamicin significantly prevented the renal injury both functionally and histologically. Ethanolic extract of flowers had a marked nitric oxide free radical scavenging effect, suggesting an antioxidative property. Two flavonoids, known for their antioxidant activity viz. kaempferol and 3, 5, 6, 7, 8-pentamethoxy flavone were isolated from the extract. The results suggested that the flowers of Pongamia pinnata had a protective effect against cisplatin and gentamicin induced renal injury through antioxidant property.
Original languageEnglish
Pages (from-to)58-62
Number of pages5
JournalIndian Journal of Experimental Biology
Volume41
Issue number1
Publication statusPublished - 2003
Externally publishedYes

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Millettia
Gentamicins
Cisplatin
Kidney
flavone
Wounds and Injuries
Urea
Creatinine
Antioxidants
Serum
Flavonoids
Free Radicals
Atrophy
Nitric Oxide
Necrosis
Epithelial Cells
Body Weight

Cite this

@article{3106b1bf793f4e219a2ba628b913914c,
title = "Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats",
abstract = "Ethanolic extract of flowers of Pongamia pinnata was studied for its protective effect against cisplatin and gentamicin induced renal injury in rats. When the extract (300 & 600 mg kg-1) was administered orally for 10 days following cisplatin (5 mg kg-1 ip) on day 5, toxicity of cisplatin, as measured by loss of body weight, elevated blood urea and serum creatinine declined significantly. Similarly in gentamicin (40 mg kg-1 s.c) induced renal injury, the extract (600 mg kg-1) normalized the raised blood urea and serum creatinine levels. Reversal of cisplatin and gentamicin renal cell damage as induced by tubular necrosis ie, marked congestion of the glomeruli with glomerular atrophy, degeneration of tubular epithelial cells with casts in the tubular lumen and infiltration of inflammatory cells in the interstitium was confirmed on histopathological examination. In the preventive regimen, co-administration of the extract with gentamicin significantly prevented the renal injury both functionally and histologically. Ethanolic extract of flowers had a marked nitric oxide free radical scavenging effect, suggesting an antioxidative property. Two flavonoids, known for their antioxidant activity viz. kaempferol and 3, 5, 6, 7, 8-pentamethoxy flavone were isolated from the extract. The results suggested that the flowers of Pongamia pinnata had a protective effect against cisplatin and gentamicin induced renal injury through antioxidant property.",
author = "A. Shirwaikar and S. Malini and S.C. Kumari",
note = "Cited By :76 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharm. Sciences, K M C, Manipal 576119, India; email: annie.shirwaikar@cops.manipal.edu Chemicals/CAS: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; Cisplatin, 15663-27-1; Gentamicins References: Barry, M.B., Toxic n{\'e}phropathies (2000) The Kidney, 2, p. 1563. , W.B. Saunders Company, Philadelphia; Devipriya, S., Shyamaladevi, C.S., Protective effect of quercetin in cisplatin induced cell injury in the rat kidney (1999) Indian J Pharmacol, 31, p. 422; Li, C.J., Bowmer, Yates, M.S., Amelioration of cisplatin nephrotoxicity with glycine: Dose dependency in rats (1995) J Pharm Pharmacol, 47, p. 223; Rao, M., Rao, M.N.A., Protective effect of selenomethionine against cisplatin induced renal toxicity in mice and rats (1998) J Pharm Pharmacol, 50, p. 687; Kanato, U., Kajufumi, S., Srie, T., Protective effect of cyclodextrin sulphates against gentamicin induced nephrotoxicity in rat (1992) J Pharm Pharmacol, 45, p. 745; Gilbert, D.N., Wood, C.A., Kohleep, S.J., Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity (1989) J Infect Dis, 159, p. 945; Vedavati, S., Mrudula, V., Sudhakar, A., (1997) Tribal Medicines of Chittoor District (A.P) India, p. 114. , Tirupathi, Herbal Folklore Research Centre; Harborne, J.B., (1984) Phytochemical Methods, p. 123. , Chapman and Hill, London; Mabry, J.J., Markham, K.R., Thomas, M.B., (1970) The Systematic Identification of Flavonoids, p. 41. , Springer Verlag, New York; Greggi, A.L.M., Darin, J.D., Bianchi, M.D., Protective effects of vit. C against cisplatin induced nephrotoxicity and lipid peroxidation in adult rats: A dose dependent study (2000) Pharmacol Res, 41, p. 405; Akira, H., Toshiaki, N., Masahioo, Y., Role of vasoactive substances in gentamicin induced acute renal failure (1994) Asian Nephrology, p. 395. , edited by K S Chugh (Oxford University Press, Delhi); Varley, H., Alan, H.G., Tests in renal disease (1984) Practical Clinical Biochemistry, p. 1123. , William Heinemann Medical Book Ltd, London; Kishore, B.K., Maldagu, P., Laurent, G., Acute renal failure induced by aminoglycoside antibiotics: Pathophysiology, clinical implications and development of protective measures (1994) Asian Nephrology, p. 395. , edited by K S Chugh (Oxford University Press, Delhi); Satyavati, G.V., Gupta, A.K., Neeraj, T., (1987) Medicinal Plants of India, p. 490. , Indian Council of Medicinal Research, New Delhi",
year = "2003",
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pages = "58--62",
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Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats. / Shirwaikar, A.; Malini, S.; Kumari, S.C.

In: Indian Journal of Experimental Biology, Vol. 41, No. 1, 2003, p. 58-62.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats

AU - Shirwaikar, A.

AU - Malini, S.

AU - Kumari, S.C.

N1 - Cited By :76 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharm. Sciences, K M C, Manipal 576119, India; email: annie.shirwaikar@cops.manipal.edu Chemicals/CAS: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; Cisplatin, 15663-27-1; Gentamicins References: Barry, M.B., Toxic néphropathies (2000) The Kidney, 2, p. 1563. , W.B. Saunders Company, Philadelphia; Devipriya, S., Shyamaladevi, C.S., Protective effect of quercetin in cisplatin induced cell injury in the rat kidney (1999) Indian J Pharmacol, 31, p. 422; Li, C.J., Bowmer, Yates, M.S., Amelioration of cisplatin nephrotoxicity with glycine: Dose dependency in rats (1995) J Pharm Pharmacol, 47, p. 223; Rao, M., Rao, M.N.A., Protective effect of selenomethionine against cisplatin induced renal toxicity in mice and rats (1998) J Pharm Pharmacol, 50, p. 687; Kanato, U., Kajufumi, S., Srie, T., Protective effect of cyclodextrin sulphates against gentamicin induced nephrotoxicity in rat (1992) J Pharm Pharmacol, 45, p. 745; Gilbert, D.N., Wood, C.A., Kohleep, S.J., Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity (1989) J Infect Dis, 159, p. 945; Vedavati, S., Mrudula, V., Sudhakar, A., (1997) Tribal Medicines of Chittoor District (A.P) India, p. 114. , Tirupathi, Herbal Folklore Research Centre; Harborne, J.B., (1984) Phytochemical Methods, p. 123. , Chapman and Hill, London; Mabry, J.J., Markham, K.R., Thomas, M.B., (1970) The Systematic Identification of Flavonoids, p. 41. , Springer Verlag, New York; Greggi, A.L.M., Darin, J.D., Bianchi, M.D., Protective effects of vit. C against cisplatin induced nephrotoxicity and lipid peroxidation in adult rats: A dose dependent study (2000) Pharmacol Res, 41, p. 405; Akira, H., Toshiaki, N., Masahioo, Y., Role of vasoactive substances in gentamicin induced acute renal failure (1994) Asian Nephrology, p. 395. , edited by K S Chugh (Oxford University Press, Delhi); Varley, H., Alan, H.G., Tests in renal disease (1984) Practical Clinical Biochemistry, p. 1123. , William Heinemann Medical Book Ltd, London; Kishore, B.K., Maldagu, P., Laurent, G., Acute renal failure induced by aminoglycoside antibiotics: Pathophysiology, clinical implications and development of protective measures (1994) Asian Nephrology, p. 395. , edited by K S Chugh (Oxford University Press, Delhi); Satyavati, G.V., Gupta, A.K., Neeraj, T., (1987) Medicinal Plants of India, p. 490. , Indian Council of Medicinal Research, New Delhi

PY - 2003

Y1 - 2003

N2 - Ethanolic extract of flowers of Pongamia pinnata was studied for its protective effect against cisplatin and gentamicin induced renal injury in rats. When the extract (300 & 600 mg kg-1) was administered orally for 10 days following cisplatin (5 mg kg-1 ip) on day 5, toxicity of cisplatin, as measured by loss of body weight, elevated blood urea and serum creatinine declined significantly. Similarly in gentamicin (40 mg kg-1 s.c) induced renal injury, the extract (600 mg kg-1) normalized the raised blood urea and serum creatinine levels. Reversal of cisplatin and gentamicin renal cell damage as induced by tubular necrosis ie, marked congestion of the glomeruli with glomerular atrophy, degeneration of tubular epithelial cells with casts in the tubular lumen and infiltration of inflammatory cells in the interstitium was confirmed on histopathological examination. In the preventive regimen, co-administration of the extract with gentamicin significantly prevented the renal injury both functionally and histologically. Ethanolic extract of flowers had a marked nitric oxide free radical scavenging effect, suggesting an antioxidative property. Two flavonoids, known for their antioxidant activity viz. kaempferol and 3, 5, 6, 7, 8-pentamethoxy flavone were isolated from the extract. The results suggested that the flowers of Pongamia pinnata had a protective effect against cisplatin and gentamicin induced renal injury through antioxidant property.

AB - Ethanolic extract of flowers of Pongamia pinnata was studied for its protective effect against cisplatin and gentamicin induced renal injury in rats. When the extract (300 & 600 mg kg-1) was administered orally for 10 days following cisplatin (5 mg kg-1 ip) on day 5, toxicity of cisplatin, as measured by loss of body weight, elevated blood urea and serum creatinine declined significantly. Similarly in gentamicin (40 mg kg-1 s.c) induced renal injury, the extract (600 mg kg-1) normalized the raised blood urea and serum creatinine levels. Reversal of cisplatin and gentamicin renal cell damage as induced by tubular necrosis ie, marked congestion of the glomeruli with glomerular atrophy, degeneration of tubular epithelial cells with casts in the tubular lumen and infiltration of inflammatory cells in the interstitium was confirmed on histopathological examination. In the preventive regimen, co-administration of the extract with gentamicin significantly prevented the renal injury both functionally and histologically. Ethanolic extract of flowers had a marked nitric oxide free radical scavenging effect, suggesting an antioxidative property. Two flavonoids, known for their antioxidant activity viz. kaempferol and 3, 5, 6, 7, 8-pentamethoxy flavone were isolated from the extract. The results suggested that the flowers of Pongamia pinnata had a protective effect against cisplatin and gentamicin induced renal injury through antioxidant property.

M3 - Article

VL - 41

SP - 58

EP - 62

JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 1

ER -