Protein profile analysis of cellular samples from the cervix for the objective diagnosis of cervical cancer using HPLC-LIF

Sujatha Bhat, Ajeetkumar Patil, Lavanya Rai, V. B. Kartha, C. Santhosh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Protein profiles of cytologic samples from the cervix were studied using High Performance Liquid Chromatographic (HPLC) separation combined with ultra-sensitive laser induced fluorescence (LIF) detection. HPLC-LIF protein profiles of samples from clinically normal subjects, individuals suffering from cervical cancer (different stages), and subjects who had other gynecological problems related to cervix, like erosion of cervix and Nabothian cyst, but no malignancy, were subjected to Principal Component Analysis (PCA). The application of HPLC-LIF protein profiling combined with PCA was found to be a highly efficient method for discrimination of different classes of samples with high sensitivity and specificity. Diagnostic accuracy and optimal threshold - decision criterion - for objective discrimination were estimated using sensitivity-specificity pairs and Youden's index (J) plots.

Original languageEnglish
Pages (from-to)3225-3230
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume878
Issue number31
DOIs
Publication statusPublished - 01-12-2010

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Cervix Uteri
Uterine Cervical Neoplasms
Lasers
Fluorescence
Principal Component Analysis
Principal component analysis
Uterine Cervical Erosion
Liquids
Sensitivity and Specificity
Proteins
Cysts
Erosion
Neoplasms

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "Protein profiles of cytologic samples from the cervix were studied using High Performance Liquid Chromatographic (HPLC) separation combined with ultra-sensitive laser induced fluorescence (LIF) detection. HPLC-LIF protein profiles of samples from clinically normal subjects, individuals suffering from cervical cancer (different stages), and subjects who had other gynecological problems related to cervix, like erosion of cervix and Nabothian cyst, but no malignancy, were subjected to Principal Component Analysis (PCA). The application of HPLC-LIF protein profiling combined with PCA was found to be a highly efficient method for discrimination of different classes of samples with high sensitivity and specificity. Diagnostic accuracy and optimal threshold - decision criterion - for objective discrimination were estimated using sensitivity-specificity pairs and Youden's index (J) plots.",
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AU - Kartha, V. B.

AU - Santhosh, C.

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