Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study

Suraj Menon, Sujatha, K. Kalyan Kumar, Jacob Kurien, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, C. Santhosh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Proteomics based techniques are rapidly emerging as alternative techniques to conventional histo-pathological methods for detection and diagnosis of cancers. Tumor markers are of considerable importance in the study of various cancers. A study of various changes in the protein profile associated with breast cancer will facilitate a better understanding of the various dynamic changes associated with the disease. In our study we have used High Performance Liquid Chromatography coupled with highly sensitive Laser Induced Fluorescence for recording the protein profiles of breast tissue homogenates. The protein profiles were recorded from pathologically certified normal as well as malignant breast tissue samples. The recorded protein profiles were studied by using Principal Component Analysis. Good discrimination of normal, benign and malignant samples was achieved in this pilot study.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems V
Volume6430
DOIs
Publication statusPublished - 2007
EventAdvanced Biomedical and Clinical Diagnostic Systems V - San Jose, CA, United States
Duration: 21-01-200727-01-2007

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic Systems V
CountryUnited States
CitySan Jose, CA
Period21-01-0727-01-07

Fingerprint

Tissue
Proteins
High performance liquid chromatography
Principal component analysis
Fluorescence
Lasers
Tumor Biomarkers
Proteomics

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Menon, S., Sujatha, Kumar, K. K., Kurien, J., Krishnanand, B. R., Mahato, K. K., ... Santhosh, C. (2007). Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study. In Advanced Biomedical and Clinical Diagnostic Systems V (Vol. 6430). [64300W] https://doi.org/10.1117/12.699935
Menon, Suraj ; Sujatha ; Kumar, K. Kalyan ; Kurien, Jacob ; Krishnanand, B. R. ; Mahato, K. K. ; George, Sajan D. ; Kartha, V. B. ; Santhosh, C. / Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study. Advanced Biomedical and Clinical Diagnostic Systems V. Vol. 6430 2007.
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author = "Suraj Menon and Sujatha and Kumar, {K. Kalyan} and Jacob Kurien and Krishnanand, {B. R.} and Mahato, {K. K.} and George, {Sajan D.} and Kartha, {V. B.} and C. Santhosh",
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Menon, S, Sujatha, Kumar, KK, Kurien, J, Krishnanand, BR, Mahato, KK, George, SD, Kartha, VB & Santhosh, C 2007, Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study. in Advanced Biomedical and Clinical Diagnostic Systems V. vol. 6430, 64300W, Advanced Biomedical and Clinical Diagnostic Systems V, San Jose, CA, United States, 21-01-07. https://doi.org/10.1117/12.699935

Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study. / Menon, Suraj; Sujatha; Kumar, K. Kalyan; Kurien, Jacob; Krishnanand, B. R.; Mahato, K. K.; George, Sajan D.; Kartha, V. B.; Santhosh, C.

Advanced Biomedical and Clinical Diagnostic Systems V. Vol. 6430 2007. 64300W.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Proteomics based techniques are rapidly emerging as alternative techniques to conventional histo-pathological methods for detection and diagnosis of cancers. Tumor markers are of considerable importance in the study of various cancers. A study of various changes in the protein profile associated with breast cancer will facilitate a better understanding of the various dynamic changes associated with the disease. In our study we have used High Performance Liquid Chromatography coupled with highly sensitive Laser Induced Fluorescence for recording the protein profiles of breast tissue homogenates. The protein profiles were recorded from pathologically certified normal as well as malignant breast tissue samples. The recorded protein profiles were studied by using Principal Component Analysis. Good discrimination of normal, benign and malignant samples was achieved in this pilot study.

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Menon S, Sujatha, Kumar KK, Kurien J, Krishnanand BR, Mahato KK et al. Protein profile study of breast cancer tissues using HPLC-LIF- A pilot study. In Advanced Biomedical and Clinical Diagnostic Systems V. Vol. 6430. 2007. 64300W https://doi.org/10.1117/12.699935