Protein profile study of breast-tissue homogenates by HPLC-LIF

K. Kalyan Kumar, M. V.P. Chowdary, Stanley Mathew, Lakshmi Rao, C. Murali Krishna, Jacob Kurien

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3 Citations (Scopus)

Abstract

Proteomics is a promising approach for molecular under-standing of neoplastic processes including response to treatment. Widely used 2D-gel electrophoresis/Liquid chromatography coupled with mass spectrometry (LC-MS) are time consuming and not cost effective. We have developed a high-sensitivity (femto/subfemtomoles of protein/20 μl) High Performance Liquid Chromatography-Laser Induced Fluorescence HPLC-LIF instrument for studying protein profiles of biological samples. In this study, we have explored the feasibility of classifying breast tissues by multivariate analysis of chromatographic data. We have analyzed 13 normal, 17 malignant, 5 benign and 4 post-treatment breast-tissue homogenates. Data was analyzed by Principal Component Analysis PCA in both unsupervised and supervised modes on derivative and baseline-corrected chromatograms. Our findings suggest that PCA of derivative chromatograms gives better classification. Thus, the HPLC-LIF instrument is not only suitable for generation of chromatographic data using femto/subfemto moles of proteins but the data can also be used for objective diagnosis via multivariate analysis. Prospectively, identified fractions can be collected and analyzed by biochemical and/or MS methods.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalJournal of Biophotonics
Volume2
Issue number5
DOIs
Publication statusPublished - 01-05-2009

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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    Kalyan Kumar, K., Chowdary, M. V. P., Mathew, S., Rao, L., Murali Krishna, C., & Kurien, J. (2009). Protein profile study of breast-tissue homogenates by HPLC-LIF. Journal of Biophotonics, 2(5), 313-321. https://doi.org/10.1002/jbio.200810046