Protein profile study of clinical samples of ovarian cancer using High Performance Liquid Chromatography-Laser Induced Fluorescence (HPLC-LIF)

Sameer Kumar Singh, Remila L. Martis, Sujatha, Rani A. Bhat, Pralhad Kushtagi, Lavanya Rai, V. B. Kartha, C. Santhosh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

New techniques for the early detection of cancer are fast emerging. This is essential for more effective diagnosis and control of the disease. We have used a High Performance Liquid Chromatography-Laser Induced Fluorescence (HPLC-LIF) technique to record chromatograms of proteins in serum and ovarian tissue samples. The recorded chromatograms of normal, benign and malignant samples were analyzed using statistical (Principal Component Analysis) methods. It is shown that chromatograms of the samples can be classified into sets, and a model based on such a classification can be used to analyze protein profiles of test samples of serum and ovarian tissue for the detection of malignancies.

Original languageEnglish
Article number60920N
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6092
DOIs
Publication statusPublished - 29-06-2006

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liquid chromatography
High performance liquid chromatography
Ovarian Neoplasms
laser induced fluorescence
Lasers
Fluorescence
cancer
High Pressure Liquid Chromatography
Tissue
proteins
Proteins
profiles
Principal Component Analysis
Early Detection of Cancer
serums
Principal component analysis
Blood Proteins
principal components analysis
Serum
emerging

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

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abstract = "New techniques for the early detection of cancer are fast emerging. This is essential for more effective diagnosis and control of the disease. We have used a High Performance Liquid Chromatography-Laser Induced Fluorescence (HPLC-LIF) technique to record chromatograms of proteins in serum and ovarian tissue samples. The recorded chromatograms of normal, benign and malignant samples were analyzed using statistical (Principal Component Analysis) methods. It is shown that chromatograms of the samples can be classified into sets, and a model based on such a classification can be used to analyze protein profiles of test samples of serum and ovarian tissue for the detection of malignancies.",
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Protein profile study of clinical samples of ovarian cancer using High Performance Liquid Chromatography-Laser Induced Fluorescence (HPLC-LIF). / Singh, Sameer Kumar; Martis, Remila L.; Sujatha; Bhat, Rani A.; Kushtagi, Pralhad; Rai, Lavanya; Kartha, V. B.; Santhosh, C.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 6092, 60920N, 29.06.2006.

Research output: Contribution to journalArticle

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AU - Singh, Sameer Kumar

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AU - Bhat, Rani A.

AU - Kushtagi, Pralhad

AU - Rai, Lavanya

AU - Kartha, V. B.

AU - Santhosh, C.

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