Protein profile study of clinical samples using laser induced fluorescence as the detection method: Case of malignant and normal cervical tissues

Gopal Karemore, Sujatha, Lavanya Rai, V. B. Kartha, C. Santhosh

Research output: Contribution to journalArticle

Abstract

Protein profiles of tissue homogenates were recorded using HPLC separation and LIF detection method. The samples were collected from volunteers with clinically normal or cervical cancer conditions. It is shown that the protein profile can be classified as belonging to malignant or normal state by using hard and Fuzzy clustering methods. The study was performed to test the utility of the HPLC-LIF protein profiling method for classification of tissue samples as well as to establish a complementary method for histopathology for clinical diagnosis of the tissue as normal or malignant.

Original languageEnglish
Article number71691I
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7169
DOIs
Publication statusPublished - 08-06-2009

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Laser-induced Fluorescence
laser induced fluorescence
Lasers
Fluorescence
High-performance Liquid Chromatography
Tissue
proteins
Proteins
Protein
profiles
Fuzzy clustering
High Pressure Liquid Chromatography
Fuzzy Clustering
Profiling
Clustering Methods
Cancer
cancer
Uterine Cervical Neoplasms
Cluster Analysis
Volunteers

All Science Journal Classification (ASJC) codes

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

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