Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Sujatha, Lavanya Rai, Pratap Kumar, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, C. Santhosh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Volume6441
DOIs
Publication statusPublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: 22-01-200724-01-2007

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
CountryUnited States
CitySan Jose, CA
Period22-01-0724-01-07

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Sujatha, Rai, L., Kumar, P., Krishnanand, B. R., Mahato, K. K., George, S. D., ... Santhosh, C. (2007). Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V (Vol. 6441). [64410B] https://doi.org/10.1117/12.699711