Quality analysis of red cell and platelet concentrates obtained by the automated 'Top-and-Top' blood processing system in a developing country

Sudipta Sekhar Das, Shamee Shastry, Rajendra Chaudhary, Anupam Verma

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The efficacy of the widely practiced 'Top and Bottom' collection system has been established. We studied efficacy of our new 'Top-and-Top' automated blood component separation system with regards to quality analysis of red cell and platelet concentrates. At installation of machine 59 U of whole blood (WB) were used for its calibration and validation program. Optimum volume, leukocyte-platelet recovery and red cells loss in BC were adjusted as per recommended standards. WB showed mean volume of 510 ml and net hemoglobin content of 63 g/bag. BC recovered 91.7% and 62.7% of platelet and leukocytes, respectively, in a mean volume of 96 ml a hematocrit of 54% and mean platelet of 5.47 × 10 10 were observed in red cell and platelet concentrates, respectively. In many aspects quality of our products could not comply with the recommended European and American standards and this requires a close insight into the series of activities associated with WB collection, separation and quality control program.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalTransfusion and Apheresis Science
Issue number1
Publication statusPublished - 01-08-2008


All Science Journal Classification (ASJC) codes

  • Hematology
  • Infectious Diseases

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