Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry

Muthusamy Annamalai, ER Sanjay, HN Nagendra Prasad, M. Radhakrishna Rao, Manjunath B Joshi, Dr. Padmalatha S Rai, Satyamoorthy Kapaettu

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Abstract

Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

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Phyllanthus urinaria
Phyllanthus amarus
Phyllanthus
quantitative analysis
spectrometry
liquid chromatography
spectroscopy
Spectrum Analysis
high performance liquid chromatography
High Pressure Liquid Chromatography
Ellagic Acid
India
Lignans
liquid
ellagic acid
liquids
lignans
transgenic plant
pharmaceutical industry
medicinal plant

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@article{a487ac9b4971465fbbfe7e14bdeac78e,
title = "Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry",
abstract = "Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.",
author = "Muthusamy Annamalai and ER Sanjay and {Nagendra Prasad}, HN and {Radhakrishna Rao}, M. and Joshi, {Manjunath B} and Rai, {Dr. Padmalatha S} and Satyamoorthy Kapaettu",
year = "2017",
doi = "DOI 10.1007/s40011-017-0839-y",
language = "English",
journal = "Proceedings of the National Academy of Sciences India Section B - Biological Sciences",
issn = "0369-8211",
publisher = "Springer India",

}

TY - JOUR

T1 - Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry

AU - Annamalai, Muthusamy

AU - Sanjay, ER

AU - Nagendra Prasad, HN

AU - Radhakrishna Rao, M.

AU - Joshi, Manjunath B

AU - Rai, Dr. Padmalatha S

AU - Kapaettu, Satyamoorthy

PY - 2017

Y1 - 2017

N2 - Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

AB - Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

U2 - DOI 10.1007/s40011-017-0839-y

DO - DOI 10.1007/s40011-017-0839-y

M3 - Article

JO - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

JF - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

SN - 0369-8211

ER -