Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry

Muthusamy Annamalai, ER Sanjay, HN Nagendra Prasad, M. Radhakrishna Rao, Manjunath B Joshi, Padmalatha S Rai, Satyamoorthy Kapaettu

    Research output: Contribution to journalArticle

    Abstract

    Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

    Fingerprint

    Phyllanthus urinaria
    Phyllanthus amarus
    Phyllanthus
    quantitative analysis
    spectrometry
    liquid chromatography
    spectroscopy
    Spectrum Analysis
    high performance liquid chromatography
    High Pressure Liquid Chromatography
    Ellagic Acid
    India
    Lignans
    liquid
    ellagic acid
    liquids
    lignans
    transgenic plant
    pharmaceutical industry
    medicinal plant

    Cite this

    @article{a487ac9b4971465fbbfe7e14bdeac78e,
    title = "Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry",
    abstract = "Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.",
    author = "Muthusamy Annamalai and ER Sanjay and {Nagendra Prasad}, HN and {Radhakrishna Rao}, M. and Joshi, {Manjunath B} and Rai, {Padmalatha S} and Satyamoorthy Kapaettu",
    year = "2017",
    doi = "DOI 10.1007/s40011-017-0839-y",
    language = "English",
    journal = "Proceedings of the National Academy of Sciences India Section B - Biological Sciences",
    issn = "0369-8211",
    publisher = "Springer India",

    }

    TY - JOUR

    T1 - Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry

    AU - Annamalai, Muthusamy

    AU - Sanjay, ER

    AU - Nagendra Prasad, HN

    AU - Radhakrishna Rao, M.

    AU - Joshi, Manjunath B

    AU - Rai, Padmalatha S

    AU - Kapaettu, Satyamoorthy

    PY - 2017

    Y1 - 2017

    N2 - Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

    AB - Phyllanthus amarus Schum. & Thonn. and P. urinaria Linn. are valuable medicinal plants which are widely used in several countries for the treatment of jaundice, diabetes, cancer and other important diseases. The Phyllanthus species are well-known for their important bioactive lignans (phyllanthin, hypophyllanthin and niranthin) and an antioxidant (ellagic acid). Qualitative and quantitative analyses by high performance liquid chromatography (HPLC) and liquid chromatography coupled to mass spectrometry (LC–MS) method revealed the contents of four bioactive molecules in Phyllanthus amarus and Phyllanthus urinaria from district Udupi, Karnataka, India. The phyllanthin, hypophyllanthin and niranthin were eluted out at specific retention time of 16.008, 14.838 and 21.106 min respectively by isocratic method and ellagic acid was eluted out at 28.656 min using gradient method in HPLC. The extracts of P. amarus from three taluks in district Udupi, Karnataka, India showed higher amounts of phyllanthin, hypophyllanthin and niranthin as compared to that of P. urinaria. The ellagic acid was found to be quantitatively higher than the three lignans of P. amarus and P. urinaria. The phytochemical profiling of bioactive compounds in these two species collected from district Udupi showed significant variations in their contents. The quantification of bioactive molecules using HPLC and LC– MS is consistent and reproducible for the future studies on somaclonal variant plants with varied amount of bioactive molecules, and transgenic plants with over-expression of lignans and phenolic compounds which has higher market value in the pharmaceutical industry.

    U2 - DOI 10.1007/s40011-017-0839-y

    DO - DOI 10.1007/s40011-017-0839-y

    M3 - Article

    JO - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

    JF - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

    SN - 0369-8211

    ER -