Quantitative role of mast cells in odontogenic cystic enlargement

Shailja Chatterjeer, Sumita Mahajan, Karen Boaz, Thomas George

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aim: Mast cells have been hypothesized to play a significant role in pathogenesis of odontogenic cysts. The aim of this study was to evaluate mast cell distribution in cystic lining and the capsule to formulate a mechanism of cystic expansion. Methods: Ten formalin-fixed paraffin embedded tissue blocks each of OKC, dentigerous and radicular cysts were selected. Toluidine blue staining (1% in 1% NaCl solution) was done in 5μm thick sections and counting performed in 10 areas using an ocular grid. Areas counted were divided into 4 zones: intraepithelial, subepithelial, intermediate and deep zones (Group I, II, III and IV respectively). Statistical analysis: Mean ±S.D. was calculated in each group followed by paired 'T' test. Results: Mast cells had greatest concentration in subepithelial zone. 'T' test showed no significant differences between group I and II zones in OKC but a highly significant difference between groups I and II in dentigerous cyst. Radicular cysts showed a significant difference between groups II and III. Conclusion: Mast cell degranulation releases numerous hydrolytic enzymes that facilitate breakdown of capsular matrix increasing the hydrostatic pressure due to raised osmolality. Influx of tissue fluids results in their enlargement coupled with resorption at the bone-cyst interface.

Original languageEnglish
Pages (from-to)1662-1665
Number of pages4
JournalBrazilian Journal of Oral Sciences
Volume7
Issue number27
Publication statusPublished - 01-12-2008

Fingerprint

Mast Cells
Radicular Cyst
Dentigerous Cyst
Odontogenic Cysts
Bone Cysts
Cell Degranulation
Tolonium Chloride
Hydrostatic Pressure
Paraffin
Osmolar Concentration
Formaldehyde
Capsules
Staining and Labeling
Enzymes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Chatterjeer, S., Mahajan, S., Boaz, K., & George, T. (2008). Quantitative role of mast cells in odontogenic cystic enlargement. Brazilian Journal of Oral Sciences, 7(27), 1662-1665.
Chatterjeer, Shailja ; Mahajan, Sumita ; Boaz, Karen ; George, Thomas. / Quantitative role of mast cells in odontogenic cystic enlargement. In: Brazilian Journal of Oral Sciences. 2008 ; Vol. 7, No. 27. pp. 1662-1665.
@article{3d187266dcec4e2ebbe659b2f2984068,
title = "Quantitative role of mast cells in odontogenic cystic enlargement",
abstract = "Aim: Mast cells have been hypothesized to play a significant role in pathogenesis of odontogenic cysts. The aim of this study was to evaluate mast cell distribution in cystic lining and the capsule to formulate a mechanism of cystic expansion. Methods: Ten formalin-fixed paraffin embedded tissue blocks each of OKC, dentigerous and radicular cysts were selected. Toluidine blue staining (1{\%} in 1{\%} NaCl solution) was done in 5μm thick sections and counting performed in 10 areas using an ocular grid. Areas counted were divided into 4 zones: intraepithelial, subepithelial, intermediate and deep zones (Group I, II, III and IV respectively). Statistical analysis: Mean ±S.D. was calculated in each group followed by paired 'T' test. Results: Mast cells had greatest concentration in subepithelial zone. 'T' test showed no significant differences between group I and II zones in OKC but a highly significant difference between groups I and II in dentigerous cyst. Radicular cysts showed a significant difference between groups II and III. Conclusion: Mast cell degranulation releases numerous hydrolytic enzymes that facilitate breakdown of capsular matrix increasing the hydrostatic pressure due to raised osmolality. Influx of tissue fluids results in their enlargement coupled with resorption at the bone-cyst interface.",
author = "Shailja Chatterjeer and Sumita Mahajan and Karen Boaz and Thomas George",
year = "2008",
month = "12",
day = "1",
language = "English",
volume = "7",
pages = "1662--1665",
journal = "Brazilian Journal of Oral Sciences",
issn = "1677-3225",
publisher = "Universidade Estadual de Campinas",
number = "27",

}

Chatterjeer, S, Mahajan, S, Boaz, K & George, T 2008, 'Quantitative role of mast cells in odontogenic cystic enlargement', Brazilian Journal of Oral Sciences, vol. 7, no. 27, pp. 1662-1665.

Quantitative role of mast cells in odontogenic cystic enlargement. / Chatterjeer, Shailja; Mahajan, Sumita; Boaz, Karen; George, Thomas.

In: Brazilian Journal of Oral Sciences, Vol. 7, No. 27, 01.12.2008, p. 1662-1665.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative role of mast cells in odontogenic cystic enlargement

AU - Chatterjeer, Shailja

AU - Mahajan, Sumita

AU - Boaz, Karen

AU - George, Thomas

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Aim: Mast cells have been hypothesized to play a significant role in pathogenesis of odontogenic cysts. The aim of this study was to evaluate mast cell distribution in cystic lining and the capsule to formulate a mechanism of cystic expansion. Methods: Ten formalin-fixed paraffin embedded tissue blocks each of OKC, dentigerous and radicular cysts were selected. Toluidine blue staining (1% in 1% NaCl solution) was done in 5μm thick sections and counting performed in 10 areas using an ocular grid. Areas counted were divided into 4 zones: intraepithelial, subepithelial, intermediate and deep zones (Group I, II, III and IV respectively). Statistical analysis: Mean ±S.D. was calculated in each group followed by paired 'T' test. Results: Mast cells had greatest concentration in subepithelial zone. 'T' test showed no significant differences between group I and II zones in OKC but a highly significant difference between groups I and II in dentigerous cyst. Radicular cysts showed a significant difference between groups II and III. Conclusion: Mast cell degranulation releases numerous hydrolytic enzymes that facilitate breakdown of capsular matrix increasing the hydrostatic pressure due to raised osmolality. Influx of tissue fluids results in their enlargement coupled with resorption at the bone-cyst interface.

AB - Aim: Mast cells have been hypothesized to play a significant role in pathogenesis of odontogenic cysts. The aim of this study was to evaluate mast cell distribution in cystic lining and the capsule to formulate a mechanism of cystic expansion. Methods: Ten formalin-fixed paraffin embedded tissue blocks each of OKC, dentigerous and radicular cysts were selected. Toluidine blue staining (1% in 1% NaCl solution) was done in 5μm thick sections and counting performed in 10 areas using an ocular grid. Areas counted were divided into 4 zones: intraepithelial, subepithelial, intermediate and deep zones (Group I, II, III and IV respectively). Statistical analysis: Mean ±S.D. was calculated in each group followed by paired 'T' test. Results: Mast cells had greatest concentration in subepithelial zone. 'T' test showed no significant differences between group I and II zones in OKC but a highly significant difference between groups I and II in dentigerous cyst. Radicular cysts showed a significant difference between groups II and III. Conclusion: Mast cell degranulation releases numerous hydrolytic enzymes that facilitate breakdown of capsular matrix increasing the hydrostatic pressure due to raised osmolality. Influx of tissue fluids results in their enlargement coupled with resorption at the bone-cyst interface.

UR - http://www.scopus.com/inward/record.url?scp=60849121102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60849121102&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:60849121102

VL - 7

SP - 1662

EP - 1665

JO - Brazilian Journal of Oral Sciences

JF - Brazilian Journal of Oral Sciences

SN - 1677-3225

IS - 27

ER -