Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin

B. Vrinda, P. Uma Devi

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Orientin (Ot) and Vicenin (Vc), two water-soluble flavonoids isolated from the leaves of Indian holy basil Ocimum sanctum have shown significant protection against radiation lethality and chromosomal aberrations in vivo. In the present study the protective effect of Ot and Vc against radiation induced chromosome damage in cultured human peripheral lymphocytes was determined by micronucleus test. In order to select the most effective drug concentration, fresh whole blood was exposed to 4Gy of cobalt-60 γ-radiation with or without a 30 min pre-treatment with 6.25, 12.5, 15.0, 17.5 or 20 μM of Ot/Vc. Micronucleus (MN) assay was done by cytochalasin induced cytokinesis block method. Radiation significantly increased the MN frequency (16 times normal). Pre-treatment with either Ot or Vc at all concentrations significantly (P < 0.05-0.001) reduced the MN count in a concentration dependent manner, with the optimum effect at 17.5 μM. Therefore, fresh blood samples were incubated with/without 17.5 μM Ot/Vc for 30 min and then exposed to 0.5-4 Gy of γ-radiation. Radiation increased the MN frequency linearly (r2 = 0.99) with dose. Pre-treatment with Ot or Vc significantly (P < 0.01-0.001) reduced the MN counts to 51-67% of RT alone values, giving DMFs of 2.62 (Ot) and 2.48 (Vc). Both the compounds showed significant antioxidant activity in vitro at the above concentrations, which was significantly higher than that of DMSO at equimolar concentrations. Thus, the results demonstrate that both the flavonoids give significant protection to the human lymphocytes against the clastogenic effect of radiation at low, non-toxic concentrations. The radioprotection seems to be associated with their antioxidant activity. The clinical potential of these protectors in cancer therapy needs to be investigated.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume498
Issue number1-2
DOIs
Publication statusPublished - 15-11-2001
Externally publishedYes

Fingerprint

Radiation Protection
Human Chromosomes
Lymphocytes
Radiation
Micronucleus Tests
Flavonoids
Antioxidants
Cytochalasins
Radiation Dosage
Cytokinesis
Radiation Effects
Dimethyl Sulfoxide
Cobalt
In Vitro Techniques
orientin
vicenin
Chromosome Aberrations
Chromosomes
Water
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

@article{13cd5fd39a2f4bfa8a8e1ed7b985aa9e,
title = "Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin",
abstract = "Orientin (Ot) and Vicenin (Vc), two water-soluble flavonoids isolated from the leaves of Indian holy basil Ocimum sanctum have shown significant protection against radiation lethality and chromosomal aberrations in vivo. In the present study the protective effect of Ot and Vc against radiation induced chromosome damage in cultured human peripheral lymphocytes was determined by micronucleus test. In order to select the most effective drug concentration, fresh whole blood was exposed to 4Gy of cobalt-60 γ-radiation with or without a 30 min pre-treatment with 6.25, 12.5, 15.0, 17.5 or 20 μM of Ot/Vc. Micronucleus (MN) assay was done by cytochalasin induced cytokinesis block method. Radiation significantly increased the MN frequency (16 times normal). Pre-treatment with either Ot or Vc at all concentrations significantly (P < 0.05-0.001) reduced the MN count in a concentration dependent manner, with the optimum effect at 17.5 μM. Therefore, fresh blood samples were incubated with/without 17.5 μM Ot/Vc for 30 min and then exposed to 0.5-4 Gy of γ-radiation. Radiation increased the MN frequency linearly (r2 = 0.99) with dose. Pre-treatment with Ot or Vc significantly (P < 0.01-0.001) reduced the MN counts to 51-67{\%} of RT alone values, giving DMFs of 2.62 (Ot) and 2.48 (Vc). Both the compounds showed significant antioxidant activity in vitro at the above concentrations, which was significantly higher than that of DMSO at equimolar concentrations. Thus, the results demonstrate that both the flavonoids give significant protection to the human lymphocytes against the clastogenic effect of radiation at low, non-toxic concentrations. The radioprotection seems to be associated with their antioxidant activity. The clinical potential of these protectors in cancer therapy needs to be investigated.",
author = "B. Vrinda and {Uma Devi}, P.",
year = "2001",
month = "11",
day = "15",
doi = "10.1016/S1383-5718(01)00263-7",
language = "English",
volume = "498",
pages = "39--46",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier",
number = "1-2",

}

Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin. / Vrinda, B.; Uma Devi, P.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 498, No. 1-2, 15.11.2001, p. 39-46.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin

AU - Vrinda, B.

AU - Uma Devi, P.

PY - 2001/11/15

Y1 - 2001/11/15

N2 - Orientin (Ot) and Vicenin (Vc), two water-soluble flavonoids isolated from the leaves of Indian holy basil Ocimum sanctum have shown significant protection against radiation lethality and chromosomal aberrations in vivo. In the present study the protective effect of Ot and Vc against radiation induced chromosome damage in cultured human peripheral lymphocytes was determined by micronucleus test. In order to select the most effective drug concentration, fresh whole blood was exposed to 4Gy of cobalt-60 γ-radiation with or without a 30 min pre-treatment with 6.25, 12.5, 15.0, 17.5 or 20 μM of Ot/Vc. Micronucleus (MN) assay was done by cytochalasin induced cytokinesis block method. Radiation significantly increased the MN frequency (16 times normal). Pre-treatment with either Ot or Vc at all concentrations significantly (P < 0.05-0.001) reduced the MN count in a concentration dependent manner, with the optimum effect at 17.5 μM. Therefore, fresh blood samples were incubated with/without 17.5 μM Ot/Vc for 30 min and then exposed to 0.5-4 Gy of γ-radiation. Radiation increased the MN frequency linearly (r2 = 0.99) with dose. Pre-treatment with Ot or Vc significantly (P < 0.01-0.001) reduced the MN counts to 51-67% of RT alone values, giving DMFs of 2.62 (Ot) and 2.48 (Vc). Both the compounds showed significant antioxidant activity in vitro at the above concentrations, which was significantly higher than that of DMSO at equimolar concentrations. Thus, the results demonstrate that both the flavonoids give significant protection to the human lymphocytes against the clastogenic effect of radiation at low, non-toxic concentrations. The radioprotection seems to be associated with their antioxidant activity. The clinical potential of these protectors in cancer therapy needs to be investigated.

AB - Orientin (Ot) and Vicenin (Vc), two water-soluble flavonoids isolated from the leaves of Indian holy basil Ocimum sanctum have shown significant protection against radiation lethality and chromosomal aberrations in vivo. In the present study the protective effect of Ot and Vc against radiation induced chromosome damage in cultured human peripheral lymphocytes was determined by micronucleus test. In order to select the most effective drug concentration, fresh whole blood was exposed to 4Gy of cobalt-60 γ-radiation with or without a 30 min pre-treatment with 6.25, 12.5, 15.0, 17.5 or 20 μM of Ot/Vc. Micronucleus (MN) assay was done by cytochalasin induced cytokinesis block method. Radiation significantly increased the MN frequency (16 times normal). Pre-treatment with either Ot or Vc at all concentrations significantly (P < 0.05-0.001) reduced the MN count in a concentration dependent manner, with the optimum effect at 17.5 μM. Therefore, fresh blood samples were incubated with/without 17.5 μM Ot/Vc for 30 min and then exposed to 0.5-4 Gy of γ-radiation. Radiation increased the MN frequency linearly (r2 = 0.99) with dose. Pre-treatment with Ot or Vc significantly (P < 0.01-0.001) reduced the MN counts to 51-67% of RT alone values, giving DMFs of 2.62 (Ot) and 2.48 (Vc). Both the compounds showed significant antioxidant activity in vitro at the above concentrations, which was significantly higher than that of DMSO at equimolar concentrations. Thus, the results demonstrate that both the flavonoids give significant protection to the human lymphocytes against the clastogenic effect of radiation at low, non-toxic concentrations. The radioprotection seems to be associated with their antioxidant activity. The clinical potential of these protectors in cancer therapy needs to be investigated.

UR - http://www.scopus.com/inward/record.url?scp=0035889594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035889594&partnerID=8YFLogxK

U2 - 10.1016/S1383-5718(01)00263-7

DO - 10.1016/S1383-5718(01)00263-7

M3 - Article

C2 - 11673069

AN - SCOPUS:0035889594

VL - 498

SP - 39

EP - 46

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

ER -