Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation

B. Nageshwar Rao, B. S.Satish Rao, B. Kiran Aithal, M. R.Sunil Kumar

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The radioprotective effect and antigenotoxic potential of phenolic alkanone, Zingerone (ZO) were investigated in Swiss albino mice exposed to gamma radiation. To study the optimum dose for radiation protection, mice were administered with ZO (10-100 mg/kg b.wt.), once daily for five consecutive days. One hour after the last administration of ZO on the fifth day, animals were whole body exposed to 10 Gy gamma radiations. The radioprotective potential was assessed using animal survival at an optimal ZO dose of 20 mg/kg b.wt., administered prior to 7-11 Gy. Further, the radioprotective potential of ZO was also analyzed by haemopoietic stem cell survival (CFU) assay, mouse bone marrow micronucleus test and histological observations of intestinal and bone marrow damage. Effect of ZO pretreatment on radiation-induced changes in glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPx) levels was also analyzed. ZO treatment resulted increase in the LD50/30 by 1.8 Gy (dose reduction factor = 1.2). The number of spleen colonies after whole body irradiation of mice (4.5 or 7.5 Gy) was increased when ZO was administered 1 h prior to irradiation. The histological observations indicated a decline in the villus height and crypt number with an increase in goblet and dead cell population in the irradiated group, which was normalized by pretreatment with ZO. A significant (p < 0.001) reduction in micronucleated polychromatic, normochromatic erythrocytes, increased PCE/NCE ratio, increase in the GSH, GST, SOD, CAT and decreased LPx levels were observed in ZO pretreated group when compared to the irradiated animals. Our findings demonstrate the potential of ZO in mitigating radiation-induced mortality and cytogenetic damage, which may be attributed to inhibition radiation-induced decline in the endogenous antioxidant levels and scavenging of radiation-induced free radicals.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume677
Issue number1-2
DOIs
Publication statusPublished - 06-2009

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Whole-Body Irradiation
Gamma Rays
Radiation
Glutathione Transferase
Catalase
Lipid Peroxidation
Superoxide Dismutase
zingerone
Bone Marrow
Radiation Dosage
Micronucleus Tests
Radiation Protection
Goblet Cells
Lethal Dose 50
Cytogenetics
Free Radicals
Glutathione
Cell Survival
Stem Cells
Spleen

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation",
abstract = "The radioprotective effect and antigenotoxic potential of phenolic alkanone, Zingerone (ZO) were investigated in Swiss albino mice exposed to gamma radiation. To study the optimum dose for radiation protection, mice were administered with ZO (10-100 mg/kg b.wt.), once daily for five consecutive days. One hour after the last administration of ZO on the fifth day, animals were whole body exposed to 10 Gy gamma radiations. The radioprotective potential was assessed using animal survival at an optimal ZO dose of 20 mg/kg b.wt., administered prior to 7-11 Gy. Further, the radioprotective potential of ZO was also analyzed by haemopoietic stem cell survival (CFU) assay, mouse bone marrow micronucleus test and histological observations of intestinal and bone marrow damage. Effect of ZO pretreatment on radiation-induced changes in glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPx) levels was also analyzed. ZO treatment resulted increase in the LD50/30 by 1.8 Gy (dose reduction factor = 1.2). The number of spleen colonies after whole body irradiation of mice (4.5 or 7.5 Gy) was increased when ZO was administered 1 h prior to irradiation. The histological observations indicated a decline in the villus height and crypt number with an increase in goblet and dead cell population in the irradiated group, which was normalized by pretreatment with ZO. A significant (p < 0.001) reduction in micronucleated polychromatic, normochromatic erythrocytes, increased PCE/NCE ratio, increase in the GSH, GST, SOD, CAT and decreased LPx levels were observed in ZO pretreated group when compared to the irradiated animals. Our findings demonstrate the potential of ZO in mitigating radiation-induced mortality and cytogenetic damage, which may be attributed to inhibition radiation-induced decline in the endogenous antioxidant levels and scavenging of radiation-induced free radicals.",
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Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation. / Rao, B. Nageshwar; Rao, B. S.Satish; Aithal, B. Kiran; Kumar, M. R.Sunil.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 677, No. 1-2, 06.2009, p. 33-41.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Rao, B. Nageshwar

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AU - Kumar, M. R.Sunil

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