Radioprotective effect of abana, a polyherbal drug following total body irradiation

M.S. Baliga, G.C. Jagetia, P. Venkatesh, R. Reddy, J.N. Ulloor

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Effects of 20 mg/kg body weight of abana (ABE) on radiation-induced sickness and mortality in mice exposed to 7 Gy to 12 Gy of gamma irradiation were studied. Treatment of mice with abana 1 h before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls (double distilled water, DDW). Abana provided protection against both the gastrointestinal and haemopoietic deaths. However, animals of both the ABE+irradiation and DDW+irradiation groups did not survive up to 30 days post-irradiation beyond 11 Gy irradiation. The LD50/30 was found to be 8.5 Gy for the DDW+irradiation group and 10.3 Gy for ABE+irradiation group. The administration of abana resulted in an increase in radiation tolerance by 1.8 Gy, and the dose modification factor (DMF) was found to be 1.2. The irradiation of animals resulted in a dose dependent elevation in lipid peroxidation, and a reduction in glutathione (GSH) concentration on day 31 post-irradiation. Treatment of mice with abana before irradiation caused a significant depletion in lipid peroxidation followed by a significant elevation in GSH concentration in the liver of mice at day 31 post-irradiation. Abana scavenged •OH, DPPH, ABTS•+ and NO• in a concentration dependent manner in vitro. Our results indicate that the radioprotective activity of abana may be due to free radical scavenging and increased GSH level in irradiated mice.
Original languageEnglish
Pages (from-to)1027-1035
Number of pages9
JournalBritish Journal of Radiology
Volume77
Issue number924
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Whole-Body Irradiation
Pharmaceutical Preparations
Radiation Injuries
Lipid Peroxidation
Water
Mortality
Radiation Tolerance
Lethal Dose 50
abana
Free Radicals
Glutathione
Body Weight
Liver
Therapeutics

Cite this

Baliga, M. S., Jagetia, G. C., Venkatesh, P., Reddy, R., & Ulloor, J. N. (2004). Radioprotective effect of abana, a polyherbal drug following total body irradiation. British Journal of Radiology, 77(924), 1027-1035. https://doi.org/10.1259/bjr/83720350
Baliga, M.S. ; Jagetia, G.C. ; Venkatesh, P. ; Reddy, R. ; Ulloor, J.N. / Radioprotective effect of abana, a polyherbal drug following total body irradiation. In: British Journal of Radiology. 2004 ; Vol. 77, No. 924. pp. 1027-1035.
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title = "Radioprotective effect of abana, a polyherbal drug following total body irradiation",
abstract = "Effects of 20 mg/kg body weight of abana (ABE) on radiation-induced sickness and mortality in mice exposed to 7 Gy to 12 Gy of gamma irradiation were studied. Treatment of mice with abana 1 h before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls (double distilled water, DDW). Abana provided protection against both the gastrointestinal and haemopoietic deaths. However, animals of both the ABE+irradiation and DDW+irradiation groups did not survive up to 30 days post-irradiation beyond 11 Gy irradiation. The LD50/30 was found to be 8.5 Gy for the DDW+irradiation group and 10.3 Gy for ABE+irradiation group. The administration of abana resulted in an increase in radiation tolerance by 1.8 Gy, and the dose modification factor (DMF) was found to be 1.2. The irradiation of animals resulted in a dose dependent elevation in lipid peroxidation, and a reduction in glutathione (GSH) concentration on day 31 post-irradiation. Treatment of mice with abana before irradiation caused a significant depletion in lipid peroxidation followed by a significant elevation in GSH concentration in the liver of mice at day 31 post-irradiation. Abana scavenged •OH, DPPH, ABTS•+ and NO• in a concentration dependent manner in vitro. Our results indicate that the radioprotective activity of abana may be due to free radical scavenging and increased GSH level in irradiated mice.",
author = "M.S. Baliga and G.C. Jagetia and P. Venkatesh and R. Reddy and J.N. Ulloor",
note = "Cited By :46 Export Date: 10 November 2017 CODEN: BJRAA Correspondence Address: Jagetia, G.C.; Department of Radiobiology, Kasturba Medical College, Manipal-576 104 Karnataka, India Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; abana, 105863-96-5; glutathione, 70-18-8; nitric oxide, 10102-43-9; abana, 105863-96-5; Antioxidants; Biphenyl Compounds; diphenyl-p-picrylhydrazyl; Free Radical Scavengers; Glutathione, 70-18-8; Hydrazines; Nitric Oxide, 10102-43-9; Plant Extracts; Radiation-Protective Agents Manufacturers: Himalaya, India References: Patt, H.M., Tyree, E.B., Straube, R.L., Smith, D.E., Cysteine protection against X-irradiation (1949) Science, 110, pp. 213-214; Sweeney, T.R., A survey of compounds from the antiradiation drug development program of the U.S. Army Medical Research and development command (1979), pp. 308-318. , Government Printing office, Washington, D.C Publication; Saini, M.R., Kumar, S., Jagetia, G.C., Saini, N., Effect of Liv. 52 against radiation sickness and mortality (1984) Ind. Pract., 37, pp. 1133-1138; Jagetia, G.C., Ganapathi, N.G., Inhibition of clastogenic effect of radiation by Liv. 52 in the bone marrow of mice (1989) Murat. Res., 224, pp. 507-510; Jagetia, G.C., Ganapathi, N.G., Treatment of mice with a herbal preparation Liv. 52. reduces the frequency of radiation induced chromosome damage in bone marrow (1991) Mutat. Res., 253, pp. 123-126; Ganapathi, N.G., Jagetia, G.C., Liv. 52 pretreatment inhibits the radiation-induced lipid peroxidation in mouse liver (1995) Curr. Sci., 68, pp. 601-603; Kumar, P.V., Kuttan, R., Kuttan, G., Radioprotective effects of Rasayanas (1996) Ind. J. Exptl. Biol., 34, pp. 848-850; Jagetia, G.C., Shrinath Baliga, M., Malagi, K.J., Sethukumar Kamath, M., The evaluation of the radioprotective effect of Triphala (an Ayurvedic rejuvenating drug) in the mice exposed to γ-radiation (2002) Phytomedicine, 9, pp. 99-108; Jagetia, G.C., Baliga, M.S., Cystone, an ayurvedic herbal drug imparts protection to the mice against the lethal effects of gamma-radiation: A preliminary study (2002) Nahrung, 46, pp. 332-336; Jagetia, G.C., Baliga, M.S., Treatment of mice with a herbal preparation mentat protects against the radiation-induced mortality (2002) Phyotherapy Res., 17, pp. 876-881; Jagetia, G.C., Baliga, M.S., The evaluation of the radioprotective effect of chyavanaprasha (an ayurvedic rasayana drug) in the mice exposed to lethal dose of γ-radiation: A preliminary study (2004) Phyotherapy Res., 18, pp. 14-18; Agarwal, B.V., Trial of abana in ischaemia heart disease and hypertension (1986) Probe, 4, pp. 314-315; Dadkar, V.N., Tahiliani, R.R., Jaguste, V.S., Damle, V.B., Dhar, H.L., Double blind comparative trial of abana and methyldopa for monotherapy of hypertension in Indian patients (1990) Japanese Heart J., 31, pp. 193-198; Tiwari, A.K., Gode, J.D., A comparative study of abana and cholesramine on serum lipids and lipoproteins in hypercholesterolemic rabbits (1990) Arogya J. Health Sci., 16, pp. 88-95; Dubey, G.P., Agrawal, A., Srivastava, V.K., Regulation of cardiovascular functions with abana (1985) Probe, 1, pp. 27-33; Basu, D.P., Joshi, R.L., Abana in cardiac neurosis and similar conditions (1986) Probe, 2, pp. 131-134; Rao, R., Abana in cardiac disease and cardiac neurosis (1988) Probe, 4, pp. 274-279; Yajnik, V.H., Acharya, H.K., Cardiovascular response to mental and physical stress: Modification by abana (1992) Probe, 4, pp. 299-303; Sankaranarayana, A., Mukherjee, S., Thusu, K., Gyawali, K., Luthra, N., Effect of abana on exercise induced tachycardia and in vitro platelet aggregation (1985) Probe, 1, pp. 25-32; Jagetia, G.C., Aruna, R., The herbal preparation abana protects against radiation-induced micronuclei in the mouse bone marrow (1997) Mutat. Res., 393, pp. 157-163; Jagetia, G.C., Baliga, M.S., Aruna, R., Rajanikant, G.K., Jain, V., Effect of abana (a herbal preparation) on the radiation-induced mortality in mice (2003) J. Ethnopharmacol., 2857, pp. 1-7; Miller, L.C., Tainter, M.L., Estimation of the ED50 and its error by means of logarithmic-probit graph paper (1944) Proc. Soc. Exp. Biol. Med., 57, p. 261; Moron, M.S., Depierre, J.W., Mannervik, B., Levels of glutathione, glutathione reductase and glutathione-s-transferase activities in rat lung and liver (1979) Biochim. Biophys. Acta, 582, pp. 67-78; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; Beuege, J.A., Aust, S.D., Microsomal lipid peroxidation (1978) Meth. Enzymol., 30, pp. 302-310; Halliwell, B., Gutteridge, J.M., Aruoma, O.I., The deoxyribose method: A simple {"}test-tube{"} Assay for determination of rate constants for reactions of hydroxyl radicals (1987) Anal. Biochem., 15, pp. 215-219; Mensor, L.L., Menezes, F.S., Leitao, G.G., Reis, A.S., Dos Santos, T.C., Coube, C.S., Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method (2001) Phytother. Res., 15, pp. 127-130; Miller, N.J., Castelluccio, C., Tijburg, L., Rice-Evans, C., The antioxidant properties of theaflavins and their gallate esters-radical scavengers or metal chelators? (1996) FEBS Letters, 392, pp. 40-44; Marcocci, L., Packer, L., Droy-Lefaix, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant action of Ginkgo biloba extract EGB 761 Methods Enzymol., 234, pp. 462-475; Sreejayan Rao, M.N., Nitric oxide scavenging by curcuminoids (1997) J. Pharm. Pharmacol., 49, pp. 105-107; Green, L.C., Wagner, D.A., Glogowski, J., Analysis of nitrate, nitrite and nitrate in biological fluids (1982) Anal. Biochem., 126, pp. 131-138; Abramowitz, M., Stegun, I.A., (1972) Handbook of Mathematical Functions, p. 925. , Library of Congress Catalog card number 65-12253, Dover Publications, Inc. New York, USA; Bond, V.P., Fliedner, T.M., Archambeau, J.O., (1965) Mammalian Radiation Lethality, , New York, USA: Academic Press; Jagetia, G.C., Baliga, M.S., Venkatesh, P., Ulloor, J.N., Influence of ginger rhizome (Zingiber officinale Rose) on survival, glutathione and lipid peroxidation in mice after whole-body exposure to gamma radiation (2003) Radiat. Res., 160, pp. 5845-5892; Nadar, T.S., Pillai, M.M., Effect of Ayurvedic medicines on beta-glucuronidase activity of Brunner's glands during recovery from cysteamine induced duodenal ulcers in rats (1989) Ind. J. Exp. Biol., 11, pp. 959-962; Badmaev, V., Nowakowski, M., Protection of epithelial cells against influenza A virus by a plant derived biological response modifier Ledretan-96 (2000) Phytother. Res., 4, pp. 245-249; Khayyal, M.T., El-Ghazaly, M.A., Kenawy, S.A., Seif-el-Nasr, M., Mahran, L.G., Kafafi, Y.A., Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination (2001) Arzneimittelforschung, 51, pp. 545-553; Phadke, S.A., Kulkarni, S.D., Screening of in vitro antibacterial activity of Terminalia chebula, Eclipta alba and Ocimum sanctum (1989) Ind. J. Med. Sci., 43, pp. 113-117; Ahmad, I., Mehmood, Z., Mohammad, F., Screening of some Indian medicinal plants for their antimicrobial properties (1908) J. Ethnopharmacol., 62, pp. 183-193; Uma Devi, P., Ganasoundari, A., Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum (1995) Ind. J. Exp. Biol., 33, pp. 205-209; Mathew, S., Kuttan, G., Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide induced toxicity (1997) J. Exp. Clin. Cancer Res., 16, pp. 407-411; Atal, C.K., Sharma, M.L., Kaul, A., Khajuria, A., Immunomodulating agents of plant origin. I: Preliminary screening (1986) J. Ethnopharmacol., 18, pp. 133-141; Warrier, P.K., Nambiar, V.P.K., Ramankutty, C., (1992) Indian Medicinal Plants (Orient Longman Ltd Hyderabad India), 5, pp. 225-228; Suresh, K., Vasudevan, D.M., Augmentation of murine natural killer cell and antibody dependent cytotoxicity activities by Phyllanthus emblica, a new immunomodulator (1994) J. Ethanopharmacol., 44, pp. 55-60; Rege, N.N., Thatte, U.M., Dahanukar, S.A., Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine (1999) Phytother. Res., 13, pp. 275-291; Ziauddin, M., Phansalkar, N., Patki, P., Diwanay, S., Patwardhan, B., Studies on the immunomodulatory effects of ashwagandha (1996) J. Ethnopharmacol., 50, pp. 69-76; Raleigh, J.M., Shum, F.Y., Radioprotector in model lipid membranes by hydroxyl radical scavengers: Supplementary role of α-tocopherol in scavenging secondary peroxy radicals (1983) Radioprotectors and Anticarcinogens, pp. 87-122. , Nygaard O, Simic MG, editors. New York: Academic Press; Leyko, W., Bartosz, G., Membrane effects of ionizing radiation and hyperthermia (1986) Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med., 49, pp. 743-770; Banerjee, S., Evacuee, A., Rao, A.R., Modulator influence of sandalwood oil on mouse hepatic Glutathione S-transferees activity and acid soluble sulphydryl level (1993) Cancer Letters, 68, pp. 105-109; Chitra, M., Shyamala Devi, C.S., Protective action of embelin against lipid peroxidation on tumor bearing mice (1994) Fitoterapia, 65, pp. 317-321; Bharali, R., Banerjee, S., Kumar, S.P.V., Rao, A.R., Role of some spice essential oils in modulating mouse hepatic antioxidant enzymes, lipid peroxidation and DNA sugar damage for cancer chemoprevention (1998) Proceed. International Conference on Radiation Biology: DNA Damage, Repair and Carcinogenesis, 64; Pandey, S., Sharma, M., Chaturvedi, P., Tripathi, Y.B., Protective effect of Rubia cordifolia on lipid peroxide formation in isolated rat liver homogenate (1994) Ind. J. Exp. Biol., 32, pp. 180-183; Uma Devi, P., Ganasoundari, A., Modulation of glutathione and antioxidant enzymes by Ocimum sanctum and its role in protection against radiation injury (1999) Indian J. Exper. Biol., 37, pp. 262-268; Joy, K.L., Kuttan, R., Anti-oxidant activity of selected plant extracts (1995) Amala Research Bulletin, 15, pp. 68-71; Evaluation of the radioprotective effect of bael leaf (Aegle marmelos) extract in mice Int. J. Radiat. Biol., p. 80. , (in press)",
year = "2004",
doi = "10.1259/bjr/83720350",
language = "English",
volume = "77",
pages = "1027--1035",
journal = "British Journal of Radiology",
issn = "0007-1285",
publisher = "British Institute of Radiology",
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}

Baliga, MS, Jagetia, GC, Venkatesh, P, Reddy, R & Ulloor, JN 2004, 'Radioprotective effect of abana, a polyherbal drug following total body irradiation', British Journal of Radiology, vol. 77, no. 924, pp. 1027-1035. https://doi.org/10.1259/bjr/83720350

Radioprotective effect of abana, a polyherbal drug following total body irradiation. / Baliga, M.S.; Jagetia, G.C.; Venkatesh, P.; Reddy, R.; Ulloor, J.N.

In: British Journal of Radiology, Vol. 77, No. 924, 2004, p. 1027-1035.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Radioprotective effect of abana, a polyherbal drug following total body irradiation

AU - Baliga, M.S.

AU - Jagetia, G.C.

AU - Venkatesh, P.

AU - Reddy, R.

AU - Ulloor, J.N.

N1 - Cited By :46 Export Date: 10 November 2017 CODEN: BJRAA Correspondence Address: Jagetia, G.C.; Department of Radiobiology, Kasturba Medical College, Manipal-576 104 Karnataka, India Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; abana, 105863-96-5; glutathione, 70-18-8; nitric oxide, 10102-43-9; abana, 105863-96-5; Antioxidants; Biphenyl Compounds; diphenyl-p-picrylhydrazyl; Free Radical Scavengers; Glutathione, 70-18-8; Hydrazines; Nitric Oxide, 10102-43-9; Plant Extracts; Radiation-Protective Agents Manufacturers: Himalaya, India References: Patt, H.M., Tyree, E.B., Straube, R.L., Smith, D.E., Cysteine protection against X-irradiation (1949) Science, 110, pp. 213-214; Sweeney, T.R., A survey of compounds from the antiradiation drug development program of the U.S. Army Medical Research and development command (1979), pp. 308-318. , Government Printing office, Washington, D.C Publication; Saini, M.R., Kumar, S., Jagetia, G.C., Saini, N., Effect of Liv. 52 against radiation sickness and mortality (1984) Ind. Pract., 37, pp. 1133-1138; Jagetia, G.C., Ganapathi, N.G., Inhibition of clastogenic effect of radiation by Liv. 52 in the bone marrow of mice (1989) Murat. Res., 224, pp. 507-510; Jagetia, G.C., Ganapathi, N.G., Treatment of mice with a herbal preparation Liv. 52. reduces the frequency of radiation induced chromosome damage in bone marrow (1991) Mutat. Res., 253, pp. 123-126; Ganapathi, N.G., Jagetia, G.C., Liv. 52 pretreatment inhibits the radiation-induced lipid peroxidation in mouse liver (1995) Curr. Sci., 68, pp. 601-603; Kumar, P.V., Kuttan, R., Kuttan, G., Radioprotective effects of Rasayanas (1996) Ind. J. Exptl. Biol., 34, pp. 848-850; Jagetia, G.C., Shrinath Baliga, M., Malagi, K.J., Sethukumar Kamath, M., The evaluation of the radioprotective effect of Triphala (an Ayurvedic rejuvenating drug) in the mice exposed to γ-radiation (2002) Phytomedicine, 9, pp. 99-108; Jagetia, G.C., Baliga, M.S., Cystone, an ayurvedic herbal drug imparts protection to the mice against the lethal effects of gamma-radiation: A preliminary study (2002) Nahrung, 46, pp. 332-336; Jagetia, G.C., Baliga, M.S., Treatment of mice with a herbal preparation mentat protects against the radiation-induced mortality (2002) Phyotherapy Res., 17, pp. 876-881; Jagetia, G.C., Baliga, M.S., The evaluation of the radioprotective effect of chyavanaprasha (an ayurvedic rasayana drug) in the mice exposed to lethal dose of γ-radiation: A preliminary study (2004) Phyotherapy Res., 18, pp. 14-18; Agarwal, B.V., Trial of abana in ischaemia heart disease and hypertension (1986) Probe, 4, pp. 314-315; Dadkar, V.N., Tahiliani, R.R., Jaguste, V.S., Damle, V.B., Dhar, H.L., Double blind comparative trial of abana and methyldopa for monotherapy of hypertension in Indian patients (1990) Japanese Heart J., 31, pp. 193-198; Tiwari, A.K., Gode, J.D., A comparative study of abana and cholesramine on serum lipids and lipoproteins in hypercholesterolemic rabbits (1990) Arogya J. Health Sci., 16, pp. 88-95; Dubey, G.P., Agrawal, A., Srivastava, V.K., Regulation of cardiovascular functions with abana (1985) Probe, 1, pp. 27-33; Basu, D.P., Joshi, R.L., Abana in cardiac neurosis and similar conditions (1986) Probe, 2, pp. 131-134; Rao, R., Abana in cardiac disease and cardiac neurosis (1988) Probe, 4, pp. 274-279; Yajnik, V.H., Acharya, H.K., Cardiovascular response to mental and physical stress: Modification by abana (1992) Probe, 4, pp. 299-303; Sankaranarayana, A., Mukherjee, S., Thusu, K., Gyawali, K., Luthra, N., Effect of abana on exercise induced tachycardia and in vitro platelet aggregation (1985) Probe, 1, pp. 25-32; Jagetia, G.C., Aruna, R., The herbal preparation abana protects against radiation-induced micronuclei in the mouse bone marrow (1997) Mutat. Res., 393, pp. 157-163; Jagetia, G.C., Baliga, M.S., Aruna, R., Rajanikant, G.K., Jain, V., Effect of abana (a herbal preparation) on the radiation-induced mortality in mice (2003) J. Ethnopharmacol., 2857, pp. 1-7; Miller, L.C., Tainter, M.L., Estimation of the ED50 and its error by means of logarithmic-probit graph paper (1944) Proc. Soc. Exp. Biol. Med., 57, p. 261; Moron, M.S., Depierre, J.W., Mannervik, B., Levels of glutathione, glutathione reductase and glutathione-s-transferase activities in rat lung and liver (1979) Biochim. Biophys. Acta, 582, pp. 67-78; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; Beuege, J.A., Aust, S.D., Microsomal lipid peroxidation (1978) Meth. Enzymol., 30, pp. 302-310; Halliwell, B., Gutteridge, J.M., Aruoma, O.I., The deoxyribose method: A simple "test-tube" Assay for determination of rate constants for reactions of hydroxyl radicals (1987) Anal. Biochem., 15, pp. 215-219; Mensor, L.L., Menezes, F.S., Leitao, G.G., Reis, A.S., Dos Santos, T.C., Coube, C.S., Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method (2001) Phytother. Res., 15, pp. 127-130; Miller, N.J., Castelluccio, C., Tijburg, L., Rice-Evans, C., The antioxidant properties of theaflavins and their gallate esters-radical scavengers or metal chelators? (1996) FEBS Letters, 392, pp. 40-44; Marcocci, L., Packer, L., Droy-Lefaix, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant action of Ginkgo biloba extract EGB 761 Methods Enzymol., 234, pp. 462-475; Sreejayan Rao, M.N., Nitric oxide scavenging by curcuminoids (1997) J. Pharm. Pharmacol., 49, pp. 105-107; Green, L.C., Wagner, D.A., Glogowski, J., Analysis of nitrate, nitrite and nitrate in biological fluids (1982) Anal. Biochem., 126, pp. 131-138; Abramowitz, M., Stegun, I.A., (1972) Handbook of Mathematical Functions, p. 925. , Library of Congress Catalog card number 65-12253, Dover Publications, Inc. New York, USA; Bond, V.P., Fliedner, T.M., Archambeau, J.O., (1965) Mammalian Radiation Lethality, , New York, USA: Academic Press; Jagetia, G.C., Baliga, M.S., Venkatesh, P., Ulloor, J.N., Influence of ginger rhizome (Zingiber officinale Rose) on survival, glutathione and lipid peroxidation in mice after whole-body exposure to gamma radiation (2003) Radiat. Res., 160, pp. 5845-5892; Nadar, T.S., Pillai, M.M., Effect of Ayurvedic medicines on beta-glucuronidase activity of Brunner's glands during recovery from cysteamine induced duodenal ulcers in rats (1989) Ind. J. Exp. Biol., 11, pp. 959-962; Badmaev, V., Nowakowski, M., Protection of epithelial cells against influenza A virus by a plant derived biological response modifier Ledretan-96 (2000) Phytother. Res., 4, pp. 245-249; Khayyal, M.T., El-Ghazaly, M.A., Kenawy, S.A., Seif-el-Nasr, M., Mahran, L.G., Kafafi, Y.A., Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination (2001) Arzneimittelforschung, 51, pp. 545-553; Phadke, S.A., Kulkarni, S.D., Screening of in vitro antibacterial activity of Terminalia chebula, Eclipta alba and Ocimum sanctum (1989) Ind. J. Med. Sci., 43, pp. 113-117; Ahmad, I., Mehmood, Z., Mohammad, F., Screening of some Indian medicinal plants for their antimicrobial properties (1908) J. Ethnopharmacol., 62, pp. 183-193; Uma Devi, P., Ganasoundari, A., Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum (1995) Ind. J. Exp. Biol., 33, pp. 205-209; Mathew, S., Kuttan, G., Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide induced toxicity (1997) J. Exp. Clin. Cancer Res., 16, pp. 407-411; Atal, C.K., Sharma, M.L., Kaul, A., Khajuria, A., Immunomodulating agents of plant origin. I: Preliminary screening (1986) J. Ethnopharmacol., 18, pp. 133-141; Warrier, P.K., Nambiar, V.P.K., Ramankutty, C., (1992) Indian Medicinal Plants (Orient Longman Ltd Hyderabad India), 5, pp. 225-228; Suresh, K., Vasudevan, D.M., Augmentation of murine natural killer cell and antibody dependent cytotoxicity activities by Phyllanthus emblica, a new immunomodulator (1994) J. Ethanopharmacol., 44, pp. 55-60; Rege, N.N., Thatte, U.M., Dahanukar, S.A., Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine (1999) Phytother. Res., 13, pp. 275-291; Ziauddin, M., Phansalkar, N., Patki, P., Diwanay, S., Patwardhan, B., Studies on the immunomodulatory effects of ashwagandha (1996) J. Ethnopharmacol., 50, pp. 69-76; Raleigh, J.M., Shum, F.Y., Radioprotector in model lipid membranes by hydroxyl radical scavengers: Supplementary role of α-tocopherol in scavenging secondary peroxy radicals (1983) Radioprotectors and Anticarcinogens, pp. 87-122. , Nygaard O, Simic MG, editors. New York: Academic Press; Leyko, W., Bartosz, G., Membrane effects of ionizing radiation and hyperthermia (1986) Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med., 49, pp. 743-770; Banerjee, S., Evacuee, A., Rao, A.R., Modulator influence of sandalwood oil on mouse hepatic Glutathione S-transferees activity and acid soluble sulphydryl level (1993) Cancer Letters, 68, pp. 105-109; Chitra, M., Shyamala Devi, C.S., Protective action of embelin against lipid peroxidation on tumor bearing mice (1994) Fitoterapia, 65, pp. 317-321; Bharali, R., Banerjee, S., Kumar, S.P.V., Rao, A.R., Role of some spice essential oils in modulating mouse hepatic antioxidant enzymes, lipid peroxidation and DNA sugar damage for cancer chemoprevention (1998) Proceed. International Conference on Radiation Biology: DNA Damage, Repair and Carcinogenesis, 64; Pandey, S., Sharma, M., Chaturvedi, P., Tripathi, Y.B., Protective effect of Rubia cordifolia on lipid peroxide formation in isolated rat liver homogenate (1994) Ind. J. Exp. Biol., 32, pp. 180-183; Uma Devi, P., Ganasoundari, A., Modulation of glutathione and antioxidant enzymes by Ocimum sanctum and its role in protection against radiation injury (1999) Indian J. Exper. Biol., 37, pp. 262-268; Joy, K.L., Kuttan, R., Anti-oxidant activity of selected plant extracts (1995) Amala Research Bulletin, 15, pp. 68-71; Evaluation of the radioprotective effect of bael leaf (Aegle marmelos) extract in mice Int. J. Radiat. Biol., p. 80. , (in press)

PY - 2004

Y1 - 2004

N2 - Effects of 20 mg/kg body weight of abana (ABE) on radiation-induced sickness and mortality in mice exposed to 7 Gy to 12 Gy of gamma irradiation were studied. Treatment of mice with abana 1 h before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls (double distilled water, DDW). Abana provided protection against both the gastrointestinal and haemopoietic deaths. However, animals of both the ABE+irradiation and DDW+irradiation groups did not survive up to 30 days post-irradiation beyond 11 Gy irradiation. The LD50/30 was found to be 8.5 Gy for the DDW+irradiation group and 10.3 Gy for ABE+irradiation group. The administration of abana resulted in an increase in radiation tolerance by 1.8 Gy, and the dose modification factor (DMF) was found to be 1.2. The irradiation of animals resulted in a dose dependent elevation in lipid peroxidation, and a reduction in glutathione (GSH) concentration on day 31 post-irradiation. Treatment of mice with abana before irradiation caused a significant depletion in lipid peroxidation followed by a significant elevation in GSH concentration in the liver of mice at day 31 post-irradiation. Abana scavenged •OH, DPPH, ABTS•+ and NO• in a concentration dependent manner in vitro. Our results indicate that the radioprotective activity of abana may be due to free radical scavenging and increased GSH level in irradiated mice.

AB - Effects of 20 mg/kg body weight of abana (ABE) on radiation-induced sickness and mortality in mice exposed to 7 Gy to 12 Gy of gamma irradiation were studied. Treatment of mice with abana 1 h before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls (double distilled water, DDW). Abana provided protection against both the gastrointestinal and haemopoietic deaths. However, animals of both the ABE+irradiation and DDW+irradiation groups did not survive up to 30 days post-irradiation beyond 11 Gy irradiation. The LD50/30 was found to be 8.5 Gy for the DDW+irradiation group and 10.3 Gy for ABE+irradiation group. The administration of abana resulted in an increase in radiation tolerance by 1.8 Gy, and the dose modification factor (DMF) was found to be 1.2. The irradiation of animals resulted in a dose dependent elevation in lipid peroxidation, and a reduction in glutathione (GSH) concentration on day 31 post-irradiation. Treatment of mice with abana before irradiation caused a significant depletion in lipid peroxidation followed by a significant elevation in GSH concentration in the liver of mice at day 31 post-irradiation. Abana scavenged •OH, DPPH, ABTS•+ and NO• in a concentration dependent manner in vitro. Our results indicate that the radioprotective activity of abana may be due to free radical scavenging and increased GSH level in irradiated mice.

U2 - 10.1259/bjr/83720350

DO - 10.1259/bjr/83720350

M3 - Article

VL - 77

SP - 1027

EP - 1035

JO - British Journal of Radiology

JF - British Journal of Radiology

SN - 0007-1285

IS - 924

ER -