Raman tweezers spectroscopy of live, single red and white blood cells

Aseefhali Bankapur, Elsa Zachariah, Santhosh Chidangil, Manna Valiathan, Deepak Mathur

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

An optical trap has been combined with a Raman spectrometer to make high-resolution measurements of Raman spectra of optically-immobilized, single, live red (RBC) and white blood cells (WBC) under physiological conditions. Tightly-focused, near infrared wavelength light (1064 nm) is utilized for trapping of single cells and 785 nm light is used for Raman excitation at low levels of incident power (few mW). Raman spectra of RBC recorded using this high-sensitivity, dual-wavelength apparatus has enabled identification of several additional lines; the hitherto-unreported lines originate purely from hemoglobin molecules. Raman spectra of single granulocytes and lymphocytes are interpreted on the basis of standard protein and nucleic acid vibrational spectroscopy data. The richness of the measured spectrum illustrates that Raman studies of live cells in suspension are more informative than conventional micro-Raman studies where the cells are chemically bound to a glass cover slip.

Original languageEnglish
Article numbere10427
JournalPLoS One
Volume5
Issue number4
DOIs
Publication statusPublished - 14-09-2010

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Raman spectroscopy
Raman Spectrum Analysis
Raman scattering
spectroscopy
leukocytes
Leukocytes
Blood
erythrocytes
Erythrocytes
Cells
Spectroscopy
Optical Tweezers
wavelengths
Light
Wavelength
Vibrational spectroscopy
Lymphocytes
Granulocytes
Nucleic Acids
Glass

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

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Raman tweezers spectroscopy of live, single red and white blood cells. / Bankapur, Aseefhali; Zachariah, Elsa; Chidangil, Santhosh; Valiathan, Manna; Mathur, Deepak.

In: PLoS One, Vol. 5, No. 4, e10427, 14.09.2010.

Research output: Contribution to journalArticle

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AU - Bankapur, Aseefhali

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AU - Mathur, Deepak

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