Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

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Abstract

Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

Original languageEnglish
Title of host publicationSecond International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
PublisherSPIE
Volume10150
ISBN (Electronic)9781510607514
DOIs
Publication statusPublished - 01-01-2016
Event2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 - Legian - Kuta, Bali, Indonesia
Duration: 24-08-201625-08-2016

Conference

Conference2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
CountryIndonesia
CityLegian - Kuta, Bali
Period24-08-1625-08-16

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Barkur, S., Bankapur, A., & Chidangil, S. (2016). Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. In Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 (Vol. 10150). [1015016] SPIE. https://doi.org/10.1117/12.2248461