Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

Original languageEnglish
Title of host publicationSecond International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
PublisherSPIE
Volume10150
ISBN (Electronic)9781510607514
DOIs
Publication statusPublished - 01-01-2016
Event2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 - Legian - Kuta, Bali, Indonesia
Duration: 24-08-201625-08-2016

Conference

Conference2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
CountryIndonesia
CityLegian - Kuta, Bali
Period24-08-1625-08-16

Fingerprint

Oxidative Stress
Free Radicals
Oxidative stress
Erythrocyte
erythrocytes
Raman
Free radicals
free radicals
Spectroscopy
Raman spectroscopy
Hydroxyl Radical
Raman scattering
Raman Spectra
hydroxyl radicals
Blood
Cells
Red Blood Cells
Raman spectra
deoxygenation
Trigger

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Barkur, S., Bankapur, A., & Chidangil, S. (2016). Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. In Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 (Vol. 10150). [1015016] SPIE. https://doi.org/10.1117/12.2248461
Barkur, Surekha ; Bankapur, Aseefhali ; Chidangil, Santhosh. / Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016. Vol. 10150 SPIE, 2016.
@inproceedings{efbccef26be84b289d8b9c8333599f9f,
title = "Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis",
abstract = "Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.",
author = "Surekha Barkur and Aseefhali Bankapur and Santhosh Chidangil",
year = "2016",
month = "1",
day = "1",
doi = "10.1117/12.2248461",
language = "English",
volume = "10150",
booktitle = "Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016",
publisher = "SPIE",
address = "United States",

}

Barkur, S, Bankapur, A & Chidangil, S 2016, Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. in Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016. vol. 10150, 1015016, SPIE, 2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016, Legian - Kuta, Bali, Indonesia, 24-08-16. https://doi.org/10.1117/12.2248461

Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. / Barkur, Surekha; Bankapur, Aseefhali; Chidangil, Santhosh.

Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016. Vol. 10150 SPIE, 2016. 1015016.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

AU - Barkur, Surekha

AU - Bankapur, Aseefhali

AU - Chidangil, Santhosh

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

AB - Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

UR - http://www.scopus.com/inward/record.url?scp=85007207149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007207149&partnerID=8YFLogxK

U2 - 10.1117/12.2248461

DO - 10.1117/12.2248461

M3 - Conference contribution

VL - 10150

BT - Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016

PB - SPIE

ER -

Barkur S, Bankapur A, Chidangil S. Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. In Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016. Vol. 10150. SPIE. 2016. 1015016 https://doi.org/10.1117/12.2248461