Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India

Anu Yamuna Joseph, Vidhu Sankar Babu, Sona S. Dev, Jayashree Gopalakrishnapai, M. Harish, M. D. Rajesh, S. Anisha, C. Mohankumar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes. Five out of twenty clinical samples were positive for CHIK virus. The partial sequences of the E1 and nsP1 genes of the strain, IndKL01 were highly similar to the Reunion strains and the recently isolated Indian strains. A novel substitution, A148V, was detected in the E1 gene of the isolate, IndKL02. The detection procedure used in this study was simple, sensitive and rapid (less than 4 hr). This result suggests that CHIK viruses similar to the Reunion strains, which had resulted in high morbidity and mortality rates, may have caused the recent Chikungunya outbreak in India. The effect of the variant, E1-A148V, in the virulence and the rate of transmission of the virus deserves further investigation.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalIndian Journal of Experimental Biology
Volume46
Issue number8
Publication statusPublished - 08-2008

Fingerprint

Chikungunya virus
Reunion
India
Fever
Polymerase Chain Reaction
Genes
Viral RNA
Serum
Disease Outbreaks
Virulence
Viruses
Morbidity
Mortality

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Joseph, A. Y., Sankar Babu, V., Dev, S. S., Gopalakrishnapai, J., Harish, M., Rajesh, M. D., ... Mohankumar, C. (2008). Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India. Indian Journal of Experimental Biology, 46(8), 573-578.
Joseph, Anu Yamuna ; Sankar Babu, Vidhu ; Dev, Sona S. ; Gopalakrishnapai, Jayashree ; Harish, M. ; Rajesh, M. D. ; Anisha, S. ; Mohankumar, C. / Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India. In: Indian Journal of Experimental Biology. 2008 ; Vol. 46, No. 8. pp. 573-578.
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Joseph, AY, Sankar Babu, V, Dev, SS, Gopalakrishnapai, J, Harish, M, Rajesh, MD, Anisha, S & Mohankumar, C 2008, 'Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India', Indian Journal of Experimental Biology, vol. 46, no. 8, pp. 573-578.

Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India. / Joseph, Anu Yamuna; Sankar Babu, Vidhu; Dev, Sona S.; Gopalakrishnapai, Jayashree; Harish, M.; Rajesh, M. D.; Anisha, S.; Mohankumar, C.

In: Indian Journal of Experimental Biology, Vol. 46, No. 8, 08.2008, p. 573-578.

Research output: Contribution to journalArticle

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AB - There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes. Five out of twenty clinical samples were positive for CHIK virus. The partial sequences of the E1 and nsP1 genes of the strain, IndKL01 were highly similar to the Reunion strains and the recently isolated Indian strains. A novel substitution, A148V, was detected in the E1 gene of the isolate, IndKL02. The detection procedure used in this study was simple, sensitive and rapid (less than 4 hr). This result suggests that CHIK viruses similar to the Reunion strains, which had resulted in high morbidity and mortality rates, may have caused the recent Chikungunya outbreak in India. The effect of the variant, E1-A148V, in the virulence and the rate of transmission of the virus deserves further investigation.

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