Real-time and rapid detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance setup

Jijo Lukose, Vignesh Shetty, Mamatha Ballal, Santhosh Chidangil, Rajeev K. Sinha

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1 Citation (Scopus)

Abstract

Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and real-time detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ∼1.5 × 108 and ∼1 × 106 cfu ml-1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6-7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.

Original languageEnglish
Article number075701
JournalLaser Physics Letters
Volume15
Issue number7
DOIs
Publication statusPublished - 01-07-2018

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salmonella
Salmonella
pathogens
Surface plasmon resonance
Pathogens
surface plasmon resonance
costs
Monoclonal antibodies
Self assembled monolayers
antibodies
Developing countries
Labeling
marking
Costs
Sustainable development
phosphates
Phosphates
platforms
injection
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Cite this

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abstract = "Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and real-time detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ∼1.5 × 108 and ∼1 × 106 cfu ml-1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6-7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.",
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AU - Chidangil, Santhosh

AU - Sinha, Rajeev K.

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AB - Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and real-time detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ∼1.5 × 108 and ∼1 × 106 cfu ml-1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6-7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.

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