Real time monitoring of biomolecular interactions using lab-built surface plasmon resonance (SPR) instrument

Jijo Lukose, Vinaya Kulal, C. Santhosh, Rajeev K. Sinha

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Surface Plasmon Resonance based sensors have found appreciable application in the biochemical and biological research due to its capability of real time monitoring of biomolecular interactions. In this study, we report the label free detection of low weight biological analytes in real time using a lab-built surface plasmon resonance based instrument. Real time binding of biotin to streptavidin attached on sensor chip was monitored in terms of variation in reflected light intensity. Mixed self-assembled monolayer of 1-Octanethiol and 1,10-Decanedithiol was explored as a linker layer for streptavidin attachment on metal film surface. The result shows that developed surface plasmon resonance based instrument has greater potential for detection of the low weight biological analytes as well as monitoring the biomolecular interactions in real time applications. In future this device can be used to investigate about the binding kinetics of bimolecular interactions to resolve the kinetic constants.

Original languageEnglish
Pages (from-to)1807-1810
Number of pages4
JournalAdvanced Science Letters
Volume23
Issue number3
DOIs
Publication statusPublished - 01-03-2017

Fingerprint

Real-time Monitoring
Surface Plasmon Resonance
Surface Plasmon
Surface plasmon resonance
monitoring
Streptavidin
Monitoring
interaction
Kinetics
Interaction
Self-assembled Monolayer
Sensor
Sensors
Light Intensity
Self assembled monolayers
sensor
Weights and Measures
kinetics
Labels
Resolve

All Science Journal Classification (ASJC) codes

  • Health(social science)
  • Computer Science(all)
  • Education
  • Mathematics(all)
  • Environmental Science(all)
  • Engineering(all)
  • Energy(all)

Cite this

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abstract = "Surface Plasmon Resonance based sensors have found appreciable application in the biochemical and biological research due to its capability of real time monitoring of biomolecular interactions. In this study, we report the label free detection of low weight biological analytes in real time using a lab-built surface plasmon resonance based instrument. Real time binding of biotin to streptavidin attached on sensor chip was monitored in terms of variation in reflected light intensity. Mixed self-assembled monolayer of 1-Octanethiol and 1,10-Decanedithiol was explored as a linker layer for streptavidin attachment on metal film surface. The result shows that developed surface plasmon resonance based instrument has greater potential for detection of the low weight biological analytes as well as monitoring the biomolecular interactions in real time applications. In future this device can be used to investigate about the binding kinetics of bimolecular interactions to resolve the kinetic constants.",
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Real time monitoring of biomolecular interactions using lab-built surface plasmon resonance (SPR) instrument. / Lukose, Jijo; Kulal, Vinaya; Santhosh, C.; Sinha, Rajeev K.

In: Advanced Science Letters, Vol. 23, No. 3, 01.03.2017, p. 1807-1810.

Research output: Contribution to journalArticle

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