1 Citation (Scopus)

Abstract

The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.

Original languageEnglish
Title of host publicationSPIE BioPhotonics Australasia
PublisherSPIE
Volume10013
ISBN (Electronic)9781510604346
DOIs
Publication statusPublished - 2016
EventSPIE BioPhotonics Australasia - Adelaide, Australia
Duration: 17-10-201619-10-2016

Conference

ConferenceSPIE BioPhotonics Australasia
CountryAustralia
CityAdelaide
Period17-10-1619-10-16

Fingerprint

Irradiation
markers
mice
Mouse
Tissue
Laser
irradiation
Lasers
Animals
lasers
Povidone-Iodine
animals
Anesthetics
Tissue regeneration
Proliferating Cell Nuclear Antigen
Hematoxylin
Eosine Yellowish-(YS)
Antigens
Wound Healing
Iodine

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

@inproceedings{468d8911f82b4da38f794977456bb3ff,
title = "Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice",
abstract = "The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5{\%} Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.",
author = "Bharath Rathnakar and Vijendra Prabhu and Rao, {Bola Sadashiva Satish} and Subhash Chandra and Sharada Rai and Mahato, {Krishna Kishore}",
year = "2016",
doi = "10.1117/12.2242919",
language = "English",
volume = "10013",
booktitle = "SPIE BioPhotonics Australasia",
publisher = "SPIE",
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}

Rathnakar, B, Prabhu, V, Rao, BSS, Chandra, S, Rai, S & Mahato, KK 2016, Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice. in SPIE BioPhotonics Australasia. vol. 10013, 100132Z, SPIE, SPIE BioPhotonics Australasia, Adelaide, Australia, 17-10-16. https://doi.org/10.1117/12.2242919

Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice. / Rathnakar, Bharath; Prabhu, Vijendra; Rao, Bola Sadashiva Satish; Chandra, Subhash; Rai, Sharada; Mahato, Krishna Kishore.

SPIE BioPhotonics Australasia. Vol. 10013 SPIE, 2016. 100132Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice

AU - Rathnakar, Bharath

AU - Prabhu, Vijendra

AU - Rao, Bola Sadashiva Satish

AU - Chandra, Subhash

AU - Rai, Sharada

AU - Mahato, Krishna Kishore

PY - 2016

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N2 - The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.

AB - The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.

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