Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla

Surendrababu P. Swathy, Kodsara Ramachandra Kiran, Madhura S. Rao, Krishna K. Mahato, Mattu Radhakrishna Rao, Kapaettu Satyamoorthy, Annamalai Muthusamy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Exposure to laser irradiation on seeds brings about the changes in agronomical characteristics of the plants. Solanum melongena L. var. Mattu Gulla, a variety of brinjal is of high economic value due to its unique colour and flavour. The aim of the study was to understand the influence of Helium-Neon (He-Ne) laser irradiation on agronomical characters of Solanum melongena L. var. Mattu Gulla in the field conditions. Various growth characteristics including seed germination percentage, survival rate, plant height, number of branches, and flowers and fruits were estimated during different developmental stages of the brinjal. In addition, the chlorogenic acid content of fruits obtained from the laser irradiated seeds were quantified using reversed-phase high-performance liquid chromatography (RP- HPLC). The plants from the seeds irradiated with different doses (20, 25 and 30 J/cm2) of He-Ne laser showed significant enhancement on the growth characteristics when compared to the non-irradiated control groups. He-Ne laser irradiation also improved the yield characteristics of the plants significantly in in vivo conditions in comparison with control group. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using methanolic extract of matured fruit of Mattu Gulla on HepG2 and fibroblast cell lines. The IC50 values of fruit extract from laser irradiated groups were found to be similar to non-irradiated control groups. Chlorogenic acid content was found to be higher in 20 J/cm2 and lower in 30 J/cm2 treated fruit tissue. The current study thus elucidates the role of He-Ne laser as a biostimulator on brinjal var. Mattu Gulla not only in the in vitro conditions but also in the in vivo field conditions.

Original languageEnglish
Pages (from-to)182-190
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume164
DOIs
Publication statusPublished - 01-11-2016

Fingerprint

Solanum melongena
Neon
helium-neon lasers
Chlorogenic Acid
Gas Lasers
fruits
Fruit
seeds
Seeds
acids
irradiation
Lasers
Control Groups
germination
lasers
liquid chromatography
Hep G2 Cells
fibroblasts
Reverse-Phase Chromatography
Growth

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla",
abstract = "Exposure to laser irradiation on seeds brings about the changes in agronomical characteristics of the plants. Solanum melongena L. var. Mattu Gulla, a variety of brinjal is of high economic value due to its unique colour and flavour. The aim of the study was to understand the influence of Helium-Neon (He-Ne) laser irradiation on agronomical characters of Solanum melongena L. var. Mattu Gulla in the field conditions. Various growth characteristics including seed germination percentage, survival rate, plant height, number of branches, and flowers and fruits were estimated during different developmental stages of the brinjal. In addition, the chlorogenic acid content of fruits obtained from the laser irradiated seeds were quantified using reversed-phase high-performance liquid chromatography (RP- HPLC). The plants from the seeds irradiated with different doses (20, 25 and 30 J/cm2) of He-Ne laser showed significant enhancement on the growth characteristics when compared to the non-irradiated control groups. He-Ne laser irradiation also improved the yield characteristics of the plants significantly in in vivo conditions in comparison with control group. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using methanolic extract of matured fruit of Mattu Gulla on HepG2 and fibroblast cell lines. The IC50 values of fruit extract from laser irradiated groups were found to be similar to non-irradiated control groups. Chlorogenic acid content was found to be higher in 20 J/cm2 and lower in 30 J/cm2 treated fruit tissue. The current study thus elucidates the role of He-Ne laser as a biostimulator on brinjal var. Mattu Gulla not only in the in vitro conditions but also in the in vivo field conditions.",
author = "Swathy, {Surendrababu P.} and Kiran, {Kodsara Ramachandra} and Rao, {Madhura S.} and Mahato, {Krishna K.} and Rao, {Mattu Radhakrishna} and Kapaettu Satyamoorthy and Annamalai Muthusamy",
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Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla. / Swathy, Surendrababu P.; Kiran, Kodsara Ramachandra; Rao, Madhura S.; Mahato, Krishna K.; Rao, Mattu Radhakrishna; Satyamoorthy, Kapaettu; Muthusamy, Annamalai.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 164, 01.11.2016, p. 182-190.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla

AU - Swathy, Surendrababu P.

AU - Kiran, Kodsara Ramachandra

AU - Rao, Madhura S.

AU - Mahato, Krishna K.

AU - Rao, Mattu Radhakrishna

AU - Satyamoorthy, Kapaettu

AU - Muthusamy, Annamalai

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