Restoration of Drug Sensitivity by Nitroxazepine Hydrochloride in P388 Murine Leukemia Cells Resistant to Adriamycin

M. P. Chitnis, S. G. Pradhan, K. Satyamoorthy, V. S. Basrur

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Attempts were made to reverse the acquired resistance of P388 murine leukemia utilizing non-toxic concentration of nitroxazepine hydrochloride, an antidepressant. The effect of nitroxazepine hydrochloride on the intracelluar accumulation of adriamycin and the inhibition of DNA synthesis were studied in these cells. The survival of mice bearing adriamycin-resistant P388 murine leukemia cells treated in vitro with nitroxazepine hydrochloride, adriamycin and a combination of the two drugs was also investigated. The results show that treatment of these cells with nitroxazepine hydrochloride significantly enhanced (1) the intracellular accumulation of adriamycin, (2) inhibition of DNA biosynthesis, and (3) the survival of mice transplanted with adriamycin-resistant P388 murine leukemia cells treated in vitro with a combination of nitroxazepine hydrochloride and adriamycin. The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhanced intracellular accumulation of adriamycin. The implications of the present investigations are promising, leaving hope for the utility of nitroxazepine hydrochloride in restoring drug sensitivity in adriamycin-resistant tumors.

    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalCancer Drug Delivery
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - 1987

    Fingerprint

    Leukemia P388
    Doxorubicin
    Pharmaceutical Preparations
    sintamil
    Drug Combinations
    Antidepressive Agents

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Cancer Research

    Cite this

    @article{dcc7127dbdaf494fb801bc0790304889,
    title = "Restoration of Drug Sensitivity by Nitroxazepine Hydrochloride in P388 Murine Leukemia Cells Resistant to Adriamycin",
    abstract = "Attempts were made to reverse the acquired resistance of P388 murine leukemia utilizing non-toxic concentration of nitroxazepine hydrochloride, an antidepressant. The effect of nitroxazepine hydrochloride on the intracelluar accumulation of adriamycin and the inhibition of DNA synthesis were studied in these cells. The survival of mice bearing adriamycin-resistant P388 murine leukemia cells treated in vitro with nitroxazepine hydrochloride, adriamycin and a combination of the two drugs was also investigated. The results show that treatment of these cells with nitroxazepine hydrochloride significantly enhanced (1) the intracellular accumulation of adriamycin, (2) inhibition of DNA biosynthesis, and (3) the survival of mice transplanted with adriamycin-resistant P388 murine leukemia cells treated in vitro with a combination of nitroxazepine hydrochloride and adriamycin. The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhanced intracellular accumulation of adriamycin. The implications of the present investigations are promising, leaving hope for the utility of nitroxazepine hydrochloride in restoring drug sensitivity in adriamycin-resistant tumors.",
    author = "Chitnis, {M. P.} and Pradhan, {S. G.} and K. Satyamoorthy and Basrur, {V. S.}",
    year = "1987",
    doi = "10.1089/cdd.1987.4.1",
    language = "English",
    volume = "4",
    pages = "1--9",
    journal = "Cancer Biotherapy and Radiopharmaceuticals",
    issn = "1084-9785",
    publisher = "Mary Ann Liebert Inc.",
    number = "1",

    }

    Restoration of Drug Sensitivity by Nitroxazepine Hydrochloride in P388 Murine Leukemia Cells Resistant to Adriamycin. / Chitnis, M. P.; Pradhan, S. G.; Satyamoorthy, K.; Basrur, V. S.

    In: Cancer Drug Delivery, Vol. 4, No. 1, 1987, p. 1-9.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Restoration of Drug Sensitivity by Nitroxazepine Hydrochloride in P388 Murine Leukemia Cells Resistant to Adriamycin

    AU - Chitnis, M. P.

    AU - Pradhan, S. G.

    AU - Satyamoorthy, K.

    AU - Basrur, V. S.

    PY - 1987

    Y1 - 1987

    N2 - Attempts were made to reverse the acquired resistance of P388 murine leukemia utilizing non-toxic concentration of nitroxazepine hydrochloride, an antidepressant. The effect of nitroxazepine hydrochloride on the intracelluar accumulation of adriamycin and the inhibition of DNA synthesis were studied in these cells. The survival of mice bearing adriamycin-resistant P388 murine leukemia cells treated in vitro with nitroxazepine hydrochloride, adriamycin and a combination of the two drugs was also investigated. The results show that treatment of these cells with nitroxazepine hydrochloride significantly enhanced (1) the intracellular accumulation of adriamycin, (2) inhibition of DNA biosynthesis, and (3) the survival of mice transplanted with adriamycin-resistant P388 murine leukemia cells treated in vitro with a combination of nitroxazepine hydrochloride and adriamycin. The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhanced intracellular accumulation of adriamycin. The implications of the present investigations are promising, leaving hope for the utility of nitroxazepine hydrochloride in restoring drug sensitivity in adriamycin-resistant tumors.

    AB - Attempts were made to reverse the acquired resistance of P388 murine leukemia utilizing non-toxic concentration of nitroxazepine hydrochloride, an antidepressant. The effect of nitroxazepine hydrochloride on the intracelluar accumulation of adriamycin and the inhibition of DNA synthesis were studied in these cells. The survival of mice bearing adriamycin-resistant P388 murine leukemia cells treated in vitro with nitroxazepine hydrochloride, adriamycin and a combination of the two drugs was also investigated. The results show that treatment of these cells with nitroxazepine hydrochloride significantly enhanced (1) the intracellular accumulation of adriamycin, (2) inhibition of DNA biosynthesis, and (3) the survival of mice transplanted with adriamycin-resistant P388 murine leukemia cells treated in vitro with a combination of nitroxazepine hydrochloride and adriamycin. The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhanced intracellular accumulation of adriamycin. The implications of the present investigations are promising, leaving hope for the utility of nitroxazepine hydrochloride in restoring drug sensitivity in adriamycin-resistant tumors.

    UR - http://www.scopus.com/inward/record.url?scp=0023573135&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0023573135&partnerID=8YFLogxK

    U2 - 10.1089/cdd.1987.4.1

    DO - 10.1089/cdd.1987.4.1

    M3 - Article

    C2 - 3607719

    AN - SCOPUS:0023573135

    VL - 4

    SP - 1

    EP - 9

    JO - Cancer Biotherapy and Radiopharmaceuticals

    JF - Cancer Biotherapy and Radiopharmaceuticals

    SN - 1084-9785

    IS - 1

    ER -