Reticulum vs inclusions: A learning experience in haemoglobin H disease

Research output: Contribution to journalArticle

Abstract

Haemoglobin H disease, also known as the alpha-thalassaemia is characterized by the presence of HbH inclusions in red blood cells, detectable on supra-vital stain. We present a case of a previously asymptomatic 31-year-old male, who insidiously developed anaemia and had prominent splenomegaly. Peripheral smear examination revealed microcytic hypochromic anaemia with numerous spherocytes and moderate polychromasia. In reticulocyte preparation with Brilliant cresyl blue, HbH inclusions were mistakenly identified as granulofilamentous reticulum of reticulocytes, giving a spuriously high reticulocyte percentage. After the literature review, repeat assessment was performed and with the aid of high performance liquid chromatography result, it was possible to delineate the HbH inclusions.

Original languageEnglish
Pages (from-to)ED17-ED19
JournalJournal of Clinical and Diagnostic Research
Volume9
Issue number10
DOIs
Publication statusPublished - 01-10-2015

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All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

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