Revealing molecular structure of starch with Stokes-vector based second harmonic generation microscopy

Nirmal Mazumder, Lu Yun-Xiang, Jianjun Qiu, Fu Jen Kao

    Research output: Contribution to journalArticle

    Abstract

    We report on the measurement and characterization of polarization properties of second harmonic (SH) light from starch granule using a Stokes vector based microscopy. The four-element Stokes parameters are the most complete description of the polarization state of SH light, including any depolarization effects. Various polarization parameters, such as the degree of polarization, the degree of linear polarization, the degree of circular polarization, as well as anisotropy are extracted by implementing a pixel by pixel image analysis from the 2D reconstructed SH Stokes images of starch granule. Furthermore, we implemented the technique by varying the polarization states of excitation light and recording the resulting Stokes parameters at micro-meter depths of resolution in order to investigate the molecular structure and orientation of the samples.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalJournal of Optics (India)
    DOIs
    Publication statusPublished - 20-06-2018

    Fingerprint

    starches
    Harmonic generation
    Starch
    Molecular structure
    harmonic generations
    Microscopic examination
    molecular structure
    Polarization
    microscopy
    polarization
    harmonics
    pixels
    Pixels
    circular polarization
    linear polarization
    Circular polarization
    image analysis
    Molecular orientation
    depolarization
    Depolarization

    All Science Journal Classification (ASJC) codes

    • Electronic, Optical and Magnetic Materials
    • Atomic and Molecular Physics, and Optics

    Cite this

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    title = "Revealing molecular structure of starch with Stokes-vector based second harmonic generation microscopy",
    abstract = "We report on the measurement and characterization of polarization properties of second harmonic (SH) light from starch granule using a Stokes vector based microscopy. The four-element Stokes parameters are the most complete description of the polarization state of SH light, including any depolarization effects. Various polarization parameters, such as the degree of polarization, the degree of linear polarization, the degree of circular polarization, as well as anisotropy are extracted by implementing a pixel by pixel image analysis from the 2D reconstructed SH Stokes images of starch granule. Furthermore, we implemented the technique by varying the polarization states of excitation light and recording the resulting Stokes parameters at micro-meter depths of resolution in order to investigate the molecular structure and orientation of the samples.",
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    Revealing molecular structure of starch with Stokes-vector based second harmonic generation microscopy. / Mazumder, Nirmal; Yun-Xiang, Lu; Qiu, Jianjun; Kao, Fu Jen.

    In: Journal of Optics (India), 20.06.2018, p. 1-7.

    Research output: Contribution to journalArticle

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    AU - Mazumder, Nirmal

    AU - Yun-Xiang, Lu

    AU - Qiu, Jianjun

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