Revealing starch denaturation with stokes vector based SHG microscopy

Nirmal Mazumder, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the 2D second harmonic (SH) Stokes images of starch granules. The SH signal on heating from hydrated and from dry starch differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.

Original languageEnglish
Title of host publicationInternational Conference on Fibre Optics and Photonics, Photonics 2016
PublisherOSA - The Optical Society
ISBN (Print)9781943580224
DOIs
Publication statusPublished - 21-07-2014
EventInternational Conference on Fibre Optics and Photonics, Photonics 2016 - Kanpur, India
Duration: 04-12-201608-12-2016

Conference

ConferenceInternational Conference on Fibre Optics and Photonics, Photonics 2016
CountryIndia
CityKanpur
Period04-12-1608-12-16

Fingerprint

Denaturation
Harmonic generation
Starch
Microscopic examination
Polarization
Circular polarization
Thermal processing (foods)
Amylopectins
Amylopectin
Heating
Polarimeters
Spatial distribution
Phase transitions

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

Cite this

Mazumder, N., & Kao, F. J. (2014). Revealing starch denaturation with stokes vector based SHG microscopy. In International Conference on Fibre Optics and Photonics, Photonics 2016 OSA - The Optical Society. https://doi.org/10.1364/PHOTONICS.2016.W3A.52
Mazumder, Nirmal ; Kao, Fu Jen. / Revealing starch denaturation with stokes vector based SHG microscopy. International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, 2014.
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Mazumder, N & Kao, FJ 2014, Revealing starch denaturation with stokes vector based SHG microscopy. in International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, International Conference on Fibre Optics and Photonics, Photonics 2016, Kanpur, India, 04-12-16. https://doi.org/10.1364/PHOTONICS.2016.W3A.52

Revealing starch denaturation with stokes vector based SHG microscopy. / Mazumder, Nirmal; Kao, Fu Jen.

International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, 2014.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the 2D second harmonic (SH) Stokes images of starch granules. The SH signal on heating from hydrated and from dry starch differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.

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Mazumder N, Kao FJ. Revealing starch denaturation with stokes vector based SHG microscopy. In International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society. 2014 https://doi.org/10.1364/PHOTONICS.2016.W3A.52