TY - JOUR
T1 - Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain
T2 - Simple, Sensitive and Robust Method
AU - Attari, Zenab
AU - Kumar, Lalit
AU - Mallikarjuna Rao, C.
AU - Koteshwara, K. B.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.
AB - A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.
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U2 - 10.1007/s11094-018-1804-7
DO - 10.1007/s11094-018-1804-7
M3 - Article
AN - SCOPUS:85047911779
SN - 0091-150X
VL - 52
SP - 266
EP - 270
JO - Pharmaceutical Chemistry Journal
JF - Pharmaceutical Chemistry Journal
IS - 3
ER -