Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain: Simple, Sensitive and Robust Method

Research output: Contribution to journalArticle

Abstract

A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.

Original languageEnglish
Pages (from-to)266-270
Number of pages5
JournalPharmaceutical Chemistry Journal
Volume52
Issue number3
DOIs
Publication statusPublished - 01-06-2018

Fingerprint

temozolomide
High Pressure Liquid Chromatography
Brain
formic acid
Pharmaceutical Preparations
Recovery Room
Temperature
Metronidazole
Glioma

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

@article{d46391727d8e45ca9b526fdecd00ddb4,
title = "Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain: Simple, Sensitive and Robust Method",
abstract = "A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52{\%}. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73{\%}; however, the drug was stable up to 15 days at –70°C.",
author = "Zenab Attari and Lalit Kumar and {Mallikarjuna Rao}, C. and Koteshwara, {K. B.}",
year = "2018",
month = "6",
day = "1",
doi = "10.1007/s11094-018-1804-7",
language = "English",
volume = "52",
pages = "266--270",
journal = "Pharmaceutical Chemistry Journal",
issn = "0091-150X",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain

T2 - Simple, Sensitive and Robust Method

AU - Attari, Zenab

AU - Kumar, Lalit

AU - Mallikarjuna Rao, C.

AU - Koteshwara, K. B.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.

AB - A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.

UR - http://www.scopus.com/inward/record.url?scp=85047911779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047911779&partnerID=8YFLogxK

U2 - 10.1007/s11094-018-1804-7

DO - 10.1007/s11094-018-1804-7

M3 - Article

AN - SCOPUS:85047911779

VL - 52

SP - 266

EP - 270

JO - Pharmaceutical Chemistry Journal

JF - Pharmaceutical Chemistry Journal

SN - 0091-150X

IS - 3

ER -