Role of catecholamine pathways in action of orexin b induced open field activity by nucleus accumbens in wistar rats

Rashmi Kaup Shiva, Ganaraja Bolumbu, Santosh Mayannavar, Dharnappa Poojari

Research output: Contribution to journalArticle

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Abstract

Aims: To elucidate whether exploratory behaviour changes following Orexin B infusion in NAc is mediated through catecholamines in male Wistar rats. Methods: Inbred male Wistar rats (n= 24) were divided into three groups. Control, Treated 1 (Orexin B) and Treated 2 (Orexin B antagonist) groups. Using stereotaxic method, guide cannula was set in place bilaterally to reach Nucleus Accumbens. Orexin B and its antagonist, TCS-OX2-29 were infused in separate groups of overnight fasted rats. Following open field activity, catecholamines (Dopamine, Adrenaline, and Noradrenaline) were estimated in brain tissue homogenate by ELISA. Data were expressed as mean±SEM (ANOVA; Student-Newman Keuls test,).p<0.05 were considered as statistically significant. Results: Orexin B infusion significantly increased noradrenaline levels in Nucleus accumbens when compared to controls. TCS-OX2-29 significantly decreased noradrenaline in Nucleus accumbens during open field exploration activity. Adrenaline was not altered significantly during open field activity. Conclusion: These results suggest that Orexin B, which plays a role in the regulation of motor and exploratory behaviour when infused in NAc mediates these actions through noradrenergic neurotransmission in Nucleus Accumbens.

Original languageEnglish
Pages (from-to)628-632
Number of pages5
JournalPharmacognosy Journal
Volume10
Issue number4
DOIs
Publication statusPublished - 01-01-2018

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Nucleus Accumbens
Catecholamines
Wistar Rats
Norepinephrine
Exploratory Behavior
Epinephrine
Synaptic Transmission
Orexins
Dopamine
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Students
Brain

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Shiva, Rashmi Kaup ; Bolumbu, Ganaraja ; Mayannavar, Santosh ; Poojari, Dharnappa. / Role of catecholamine pathways in action of orexin b induced open field activity by nucleus accumbens in wistar rats. In: Pharmacognosy Journal. 2018 ; Vol. 10, No. 4. pp. 628-632.
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Role of catecholamine pathways in action of orexin b induced open field activity by nucleus accumbens in wistar rats. / Shiva, Rashmi Kaup; Bolumbu, Ganaraja; Mayannavar, Santosh; Poojari, Dharnappa.

In: Pharmacognosy Journal, Vol. 10, No. 4, 01.01.2018, p. 628-632.

Research output: Contribution to journalArticle

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