Rol del alcohol diluido en la eliminación de arrugas finas desde secciones de parafina, un estudio histotécnico

Translated title of the contribution: Role of dilute alcohol in the removal of fine wrinkles from paraffin sections, a histo-technical study

Naveen Kumar, B. Satheesha Nayak, S. N. Somayaji, Sadananda Nayak

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person who needs to do section cutting. In the routine method of preparing paraffin sections, it is often encountered by the presence of artefacts like fine wrinkles or folds. Attempts have been made to remove the wrinkles by floating the sections in the warm water bath. However this method has not been able to remove all the wrinkles from the sections. We have designed a simple and reliable method, in which the paraffin sections were floated over the ethyl alcohol diluted with water (1:15) before they were placed in the water bath. Through this method, we have been able to get the wrinkle free sections of superior quality. The advantage of our method is that, it is easy to prepare the dilute alcohol and is cost effective. This method can be used by the histology and pathology technicians and the researchers.

Original languageSpanish
Pages (from-to)45-48
Number of pages4
JournalInternational Journal of Morphology
Volume30
Issue number1
DOIs
Publication statusPublished - 01-01-2012

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Paraffin
Alcohols
Baths
Water
Histology
Art
Artifacts
Ethanol
Research Personnel
Pathology
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

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title = "Rol del alcohol diluido en la eliminaci{\'o}n de arrugas finas desde secciones de parafina, un estudio histot{\'e}cnico",
abstract = "Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person who needs to do section cutting. In the routine method of preparing paraffin sections, it is often encountered by the presence of artefacts like fine wrinkles or folds. Attempts have been made to remove the wrinkles by floating the sections in the warm water bath. However this method has not been able to remove all the wrinkles from the sections. We have designed a simple and reliable method, in which the paraffin sections were floated over the ethyl alcohol diluted with water (1:15) before they were placed in the water bath. Through this method, we have been able to get the wrinkle free sections of superior quality. The advantage of our method is that, it is easy to prepare the dilute alcohol and is cost effective. This method can be used by the histology and pathology technicians and the researchers.",
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Rol del alcohol diluido en la eliminación de arrugas finas desde secciones de parafina, un estudio histotécnico. / Kumar, Naveen; Satheesha Nayak, B.; Somayaji, S. N.; Nayak, Sadananda.

In: International Journal of Morphology, Vol. 30, No. 1, 01.01.2012, p. 45-48.

Research output: Contribution to journalArticle

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