Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India

Krishn Pratap Singh, Shayan Shakeel, Namrata Naskar, Aakanksha Bharti, Asha Kaul, Shadab Anwar, Shweta Kumari, Amod Kumar, Jiv Kant Singh, Nutan Kumari, Birendra Kumar Gupta, Purwa Manna, Vishwaprakash Roy, Sneh Lata, Om P. Singh, Manoranjan Prasad Sinha, Ajay Kumar Sharma, Mohammad Sohail

Research output: Contribution to journalArticle

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Abstract

Background: The combinatorial effects of Plasmodium infection, perturbation of inflammatory responses and the dichotomic role of TNF promoter polymorphism has potential clinical and physiological relevance during pregnancy. Objective and Methods: This coordinated orchestration instigated us to investigate the circulating level of inflammatory cytokines (IL-1β TNF-α and IL-6) employing ELISA in a stratified group of samples and the plausible genetic association of TNF-α −308 G/A using PCR-RFLP/sequencing during Plasmodium vivax infection in pregnancy. Results: We observed significantly elevated concentrations of IL-1β were observed, followed by IL-6 and TNF-α in women with malaria (WWM) and in malaria in pregnancy (MIP). Further, elevated IL-1β followed by TNF-α and IL-6 were detected in the non-infected pregnancy group. The differential dynamics of inflammatory cytokine concentration during each trimester of pregnancy with and without P. vivax infection were detected. For the first time, a high level of IL-6 was observed in the first trimester of MIP and high IL-1β in healthy pregnancies. In the second trimester, however, we observed a high level of IL-1β in the MIP group compared to a sustained high level of IL-1β in the healthy pregnancy group. In the third trimester, high IL-1β was sustained in the MIP group and healthy pregnancies acquired a high TNF-α level. The genotypic distribution for the TNF-α promoter −308 G/A position was observed to be nonsignificant and mildly associated during MIP (OR = 1.4) and in WWM (OR = 1.2). Moreover, based on genotypic distribution, we observed a well-correlated and significantly elevated TNF-α concentration in the mutant homozygote genotype (AA; p = 0.001) followed by heterozygotes (GA; p = 0.0001) and ancestral genotypes (GG; p = 0.0001) in both MIP and WWM subjects. Conclusion: The observation of elevated IL-1β and IL-6 in MIP and TNF-α in WWM may be regarded as a prognostic inflammatory marker of infection and pregnancy. Most particularly, the TNF-α concentration and its polymorphic variability in the promoter region may indicate genetic susceptibility and mildly influence the risk for P. vivax infection during pregnancy and in women with malaria.

Original languageEnglish
Pages (from-to)82-93
Number of pages12
JournalMolecular Immunology
Volume97
DOIs
Publication statusPublished - 01-05-2018
Externally publishedYes

Fingerprint

Plasmodium vivax
Interleukin-1
Malaria
India
Interleukin-6
Cytokines
Pregnancy
Population
Genotype
Pregnancy Trimesters
Homozygote
Third Pregnancy Trimester
Second Pregnancy Trimester
First Pregnancy Trimester

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Singh, Krishn Pratap ; Shakeel, Shayan ; Naskar, Namrata ; Bharti, Aakanksha ; Kaul, Asha ; Anwar, Shadab ; Kumari, Shweta ; Kumar, Amod ; Singh, Jiv Kant ; Kumari, Nutan ; Gupta, Birendra Kumar ; Manna, Purwa ; Roy, Vishwaprakash ; Lata, Sneh ; Singh, Om P. ; Sinha, Manoranjan Prasad ; Sharma, Ajay Kumar ; Sohail, Mohammad. / Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India. In: Molecular Immunology. 2018 ; Vol. 97. pp. 82-93.
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title = "Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India",
abstract = "Background: The combinatorial effects of Plasmodium infection, perturbation of inflammatory responses and the dichotomic role of TNF promoter polymorphism has potential clinical and physiological relevance during pregnancy. Objective and Methods: This coordinated orchestration instigated us to investigate the circulating level of inflammatory cytokines (IL-1β TNF-α and IL-6) employing ELISA in a stratified group of samples and the plausible genetic association of TNF-α −308 G/A using PCR-RFLP/sequencing during Plasmodium vivax infection in pregnancy. Results: We observed significantly elevated concentrations of IL-1β were observed, followed by IL-6 and TNF-α in women with malaria (WWM) and in malaria in pregnancy (MIP). Further, elevated IL-1β followed by TNF-α and IL-6 were detected in the non-infected pregnancy group. The differential dynamics of inflammatory cytokine concentration during each trimester of pregnancy with and without P. vivax infection were detected. For the first time, a high level of IL-6 was observed in the first trimester of MIP and high IL-1β in healthy pregnancies. In the second trimester, however, we observed a high level of IL-1β in the MIP group compared to a sustained high level of IL-1β in the healthy pregnancy group. In the third trimester, high IL-1β was sustained in the MIP group and healthy pregnancies acquired a high TNF-α level. The genotypic distribution for the TNF-α promoter −308 G/A position was observed to be nonsignificant and mildly associated during MIP (OR = 1.4) and in WWM (OR = 1.2). Moreover, based on genotypic distribution, we observed a well-correlated and significantly elevated TNF-α concentration in the mutant homozygote genotype (AA; p = 0.001) followed by heterozygotes (GA; p = 0.0001) and ancestral genotypes (GG; p = 0.0001) in both MIP and WWM subjects. Conclusion: The observation of elevated IL-1β and IL-6 in MIP and TNF-α in WWM may be regarded as a prognostic inflammatory marker of infection and pregnancy. Most particularly, the TNF-α concentration and its polymorphic variability in the promoter region may indicate genetic susceptibility and mildly influence the risk for P. vivax infection during pregnancy and in women with malaria.",
author = "Singh, {Krishn Pratap} and Shayan Shakeel and Namrata Naskar and Aakanksha Bharti and Asha Kaul and Shadab Anwar and Shweta Kumari and Amod Kumar and Singh, {Jiv Kant} and Nutan Kumari and Gupta, {Birendra Kumar} and Purwa Manna and Vishwaprakash Roy and Sneh Lata and Singh, {Om P.} and Sinha, {Manoranjan Prasad} and Sharma, {Ajay Kumar} and Mohammad Sohail",
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language = "English",
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pages = "82--93",
journal = "Molecular Immunology",
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Singh, KP, Shakeel, S, Naskar, N, Bharti, A, Kaul, A, Anwar, S, Kumari, S, Kumar, A, Singh, JK, Kumari, N, Gupta, BK, Manna, P, Roy, V, Lata, S, Singh, OP, Sinha, MP, Sharma, AK & Sohail, M 2018, 'Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India', Molecular Immunology, vol. 97, pp. 82-93. https://doi.org/10.1016/j.molimm.2018.03.019

Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India. / Singh, Krishn Pratap; Shakeel, Shayan; Naskar, Namrata; Bharti, Aakanksha; Kaul, Asha; Anwar, Shadab; Kumari, Shweta; Kumar, Amod; Singh, Jiv Kant; Kumari, Nutan; Gupta, Birendra Kumar; Manna, Purwa; Roy, Vishwaprakash; Lata, Sneh; Singh, Om P.; Sinha, Manoranjan Prasad; Sharma, Ajay Kumar; Sohail, Mohammad.

In: Molecular Immunology, Vol. 97, 01.05.2018, p. 82-93.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Role of IL-1β IL-6 and TNF-α cytokines and TNF-α promoter variability in Plasmodium vivax infection during pregnancy in endemic population of Jharkhand, India

AU - Singh, Krishn Pratap

AU - Shakeel, Shayan

AU - Naskar, Namrata

AU - Bharti, Aakanksha

AU - Kaul, Asha

AU - Anwar, Shadab

AU - Kumari, Shweta

AU - Kumar, Amod

AU - Singh, Jiv Kant

AU - Kumari, Nutan

AU - Gupta, Birendra Kumar

AU - Manna, Purwa

AU - Roy, Vishwaprakash

AU - Lata, Sneh

AU - Singh, Om P.

AU - Sinha, Manoranjan Prasad

AU - Sharma, Ajay Kumar

AU - Sohail, Mohammad

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Background: The combinatorial effects of Plasmodium infection, perturbation of inflammatory responses and the dichotomic role of TNF promoter polymorphism has potential clinical and physiological relevance during pregnancy. Objective and Methods: This coordinated orchestration instigated us to investigate the circulating level of inflammatory cytokines (IL-1β TNF-α and IL-6) employing ELISA in a stratified group of samples and the plausible genetic association of TNF-α −308 G/A using PCR-RFLP/sequencing during Plasmodium vivax infection in pregnancy. Results: We observed significantly elevated concentrations of IL-1β were observed, followed by IL-6 and TNF-α in women with malaria (WWM) and in malaria in pregnancy (MIP). Further, elevated IL-1β followed by TNF-α and IL-6 were detected in the non-infected pregnancy group. The differential dynamics of inflammatory cytokine concentration during each trimester of pregnancy with and without P. vivax infection were detected. For the first time, a high level of IL-6 was observed in the first trimester of MIP and high IL-1β in healthy pregnancies. In the second trimester, however, we observed a high level of IL-1β in the MIP group compared to a sustained high level of IL-1β in the healthy pregnancy group. In the third trimester, high IL-1β was sustained in the MIP group and healthy pregnancies acquired a high TNF-α level. The genotypic distribution for the TNF-α promoter −308 G/A position was observed to be nonsignificant and mildly associated during MIP (OR = 1.4) and in WWM (OR = 1.2). Moreover, based on genotypic distribution, we observed a well-correlated and significantly elevated TNF-α concentration in the mutant homozygote genotype (AA; p = 0.001) followed by heterozygotes (GA; p = 0.0001) and ancestral genotypes (GG; p = 0.0001) in both MIP and WWM subjects. Conclusion: The observation of elevated IL-1β and IL-6 in MIP and TNF-α in WWM may be regarded as a prognostic inflammatory marker of infection and pregnancy. Most particularly, the TNF-α concentration and its polymorphic variability in the promoter region may indicate genetic susceptibility and mildly influence the risk for P. vivax infection during pregnancy and in women with malaria.

AB - Background: The combinatorial effects of Plasmodium infection, perturbation of inflammatory responses and the dichotomic role of TNF promoter polymorphism has potential clinical and physiological relevance during pregnancy. Objective and Methods: This coordinated orchestration instigated us to investigate the circulating level of inflammatory cytokines (IL-1β TNF-α and IL-6) employing ELISA in a stratified group of samples and the plausible genetic association of TNF-α −308 G/A using PCR-RFLP/sequencing during Plasmodium vivax infection in pregnancy. Results: We observed significantly elevated concentrations of IL-1β were observed, followed by IL-6 and TNF-α in women with malaria (WWM) and in malaria in pregnancy (MIP). Further, elevated IL-1β followed by TNF-α and IL-6 were detected in the non-infected pregnancy group. The differential dynamics of inflammatory cytokine concentration during each trimester of pregnancy with and without P. vivax infection were detected. For the first time, a high level of IL-6 was observed in the first trimester of MIP and high IL-1β in healthy pregnancies. In the second trimester, however, we observed a high level of IL-1β in the MIP group compared to a sustained high level of IL-1β in the healthy pregnancy group. In the third trimester, high IL-1β was sustained in the MIP group and healthy pregnancies acquired a high TNF-α level. The genotypic distribution for the TNF-α promoter −308 G/A position was observed to be nonsignificant and mildly associated during MIP (OR = 1.4) and in WWM (OR = 1.2). Moreover, based on genotypic distribution, we observed a well-correlated and significantly elevated TNF-α concentration in the mutant homozygote genotype (AA; p = 0.001) followed by heterozygotes (GA; p = 0.0001) and ancestral genotypes (GG; p = 0.0001) in both MIP and WWM subjects. Conclusion: The observation of elevated IL-1β and IL-6 in MIP and TNF-α in WWM may be regarded as a prognostic inflammatory marker of infection and pregnancy. Most particularly, the TNF-α concentration and its polymorphic variability in the promoter region may indicate genetic susceptibility and mildly influence the risk for P. vivax infection during pregnancy and in women with malaria.

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