Role of PCR for diagnosing Pneumocystis jirovecii pneumonia in HIV-infected individuals in a tertiary care hospital in India

Kiran Chawla, Suganthi Martena, Bimala Gurung, Chiranjay Mukhopadhyay, George K. Varghese, Indira Bairy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives : In developing countries like India, the diagnosis of Pneumocystis jirovecii infection is often made either by conventional staining or clinically. This study was planned to know the utility of polymerase chain reaction (PCR) in diagnosing Pneumocystis jirovecii pneumonia (PJP) in human immunodeficiency virus (HIV)-infected patients, to compare the PCR results with that of staining techniques and also to correlate the results with clinical condition of patients. Materials and Methods: A prospective study included 50 HIV-infected adult in-patients with symptoms of lower respiratory tract infection. Induced sputum, bronchoalveolar lavage or tracheal aspirate were proceeded for both staining and PCR for mitochondrial large subunit rRNA gene of P. jirovecii. Results: In our study PCR results correlated with staining findings in 14% (7/50) of cases. Another 20% (10/50) cases could be diagnosed only with PCR, where staining was negative for the presence of P. jirovecii. When compared with clinical evidence of disease, PCR showed 93.7% sensitivity and 94.1% specificity. Presence of dyspnea and CD 4 count showed statistical significance (P<0.05) in PCP-diagnosed patients. Conclusions: PCR can be used for early and accurate diagnosis of PCP in HIV-infected patients.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalIndian Journal of Pathology and Microbiology
Volume54
Issue number2
DOIs
Publication statusPublished - 01-04-2011

Fingerprint

Pneumocystis carinii
Pneumocystis Pneumonia
Tertiary Healthcare
Tertiary Care Centers
India
HIV
Polymerase Chain Reaction
Staining and Labeling
Pneumocystis Infections
Negative Staining
Bronchoalveolar Lavage
Sputum
rRNA Genes
Respiratory Tract Infections
Dyspnea
Developing Countries
Early Diagnosis
Prospective Studies
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

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abstract = "Objectives : In developing countries like India, the diagnosis of Pneumocystis jirovecii infection is often made either by conventional staining or clinically. This study was planned to know the utility of polymerase chain reaction (PCR) in diagnosing Pneumocystis jirovecii pneumonia (PJP) in human immunodeficiency virus (HIV)-infected patients, to compare the PCR results with that of staining techniques and also to correlate the results with clinical condition of patients. Materials and Methods: A prospective study included 50 HIV-infected adult in-patients with symptoms of lower respiratory tract infection. Induced sputum, bronchoalveolar lavage or tracheal aspirate were proceeded for both staining and PCR for mitochondrial large subunit rRNA gene of P. jirovecii. Results: In our study PCR results correlated with staining findings in 14{\%} (7/50) of cases. Another 20{\%} (10/50) cases could be diagnosed only with PCR, where staining was negative for the presence of P. jirovecii. When compared with clinical evidence of disease, PCR showed 93.7{\%} sensitivity and 94.1{\%} specificity. Presence of dyspnea and CD 4 count showed statistical significance (P<0.05) in PCP-diagnosed patients. Conclusions: PCR can be used for early and accurate diagnosis of PCP in HIV-infected patients.",
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Role of PCR for diagnosing Pneumocystis jirovecii pneumonia in HIV-infected individuals in a tertiary care hospital in India. / Chawla, Kiran; Martena, Suganthi; Gurung, Bimala; Mukhopadhyay, Chiranjay; Varghese, George K.; Bairy, Indira.

In: Indian Journal of Pathology and Microbiology, Vol. 54, No. 2, 01.04.2011, p. 326-329.

Research output: Contribution to journalArticle

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AU - Chawla, Kiran

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AU - Mukhopadhyay, Chiranjay

AU - Varghese, George K.

AU - Bairy, Indira

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