RP-HPLC estimation of rofecoxib and tizanidine in combination tablets

G. Subramanian, S. Pandey, N. Udupa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of rofecoxib and tizanidine in tablet formulations. The separation was achieved by Luna C18 column and methanol : phosphate buffer pH 3.5 (55:45 v/v) as eluent, at a flow rate of 1 ml/min. Detection was carried out at 240 nm. Valdecoxib was used as an internal standard. The retention time of rofecoxib and tizanidine was found to be 4.53 and 5.92 min, respectively. The method has been validated for linearity accuracy and precision. Linearity for tizanidine and rofecoxib were in the range of 0.6-1.4 μg/ml and 7.5-17.5 μg/ml, respectively. The mean recoveries obtained for tizanidine and rofecoxib were 98.73% and 99.70%, respectively. The developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of rofecoxib and tizanidine in tablets.

Original languageEnglish
Pages (from-to)699-701
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume66
Issue number5
Publication statusPublished - 2004

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Tablets
High Pressure Liquid Chromatography
Reverse-Phase Chromatography
Methanol
Buffers
Phosphates
rofecoxib
tizanidine

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

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abstract = "A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of rofecoxib and tizanidine in tablet formulations. The separation was achieved by Luna C18 column and methanol : phosphate buffer pH 3.5 (55:45 v/v) as eluent, at a flow rate of 1 ml/min. Detection was carried out at 240 nm. Valdecoxib was used as an internal standard. The retention time of rofecoxib and tizanidine was found to be 4.53 and 5.92 min, respectively. The method has been validated for linearity accuracy and precision. Linearity for tizanidine and rofecoxib were in the range of 0.6-1.4 μg/ml and 7.5-17.5 μg/ml, respectively. The mean recoveries obtained for tizanidine and rofecoxib were 98.73{\%} and 99.70{\%}, respectively. The developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of rofecoxib and tizanidine in tablets.",
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RP-HPLC estimation of rofecoxib and tizanidine in combination tablets. / Subramanian, G.; Pandey, S.; Udupa, N.

In: Indian Journal of Pharmaceutical Sciences, Vol. 66, No. 5, 2004, p. 699-701.

Research output: Contribution to journalArticle

TY - JOUR

T1 - RP-HPLC estimation of rofecoxib and tizanidine in combination tablets

AU - Subramanian, G.

AU - Pandey, S.

AU - Udupa, N.

PY - 2004

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N2 - A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of rofecoxib and tizanidine in tablet formulations. The separation was achieved by Luna C18 column and methanol : phosphate buffer pH 3.5 (55:45 v/v) as eluent, at a flow rate of 1 ml/min. Detection was carried out at 240 nm. Valdecoxib was used as an internal standard. The retention time of rofecoxib and tizanidine was found to be 4.53 and 5.92 min, respectively. The method has been validated for linearity accuracy and precision. Linearity for tizanidine and rofecoxib were in the range of 0.6-1.4 μg/ml and 7.5-17.5 μg/ml, respectively. The mean recoveries obtained for tizanidine and rofecoxib were 98.73% and 99.70%, respectively. The developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of rofecoxib and tizanidine in tablets.

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