Abstract

The subsequent artefact entail of extremely subtle and informal Reversed phase HPLC (RP-HPLC) technique for approximation ofMethylcobalamin in large quantity. Shimadzu LC-20- AD(Kyoto, Japan), with auto sampler and (PDA/UV) detector was used for the separation of Methylcobalamin.C18 Hypersil BDS (5.0 micron,150 x 4.6mm) column was aimed at the separation and mobile phase(MP) comprising of(55:45 v/v) Methanol:o-phosphoric acid 0.02%v/v pH 2.3 was castoffby keeping the rate offlow1 ml/minalong the effluents supervised on 223nm. linearity is undeviating having line equationf(x) =4.87512e-006*x-0.702284 with correlation coefficient(R2) of 0.997. Theprocessremained precise with 0.5% RSD(intra-day precision) & (Inter-day precision) is 0.43%. The LOD & LOQ was0.1499μg/ml and 0.4542μg/ml respectively.The establishedprocess wasvalidated forlinearity,system suitability,precision, and robustness; henceforthverifies in order that this one is exceedinglyrelevantto approximate Methylcobalamin in routine analysis.Methods name: Estimation of Methylcobalaminby HPLC.

Original languageEnglish
Pages (from-to)6-12
Number of pages7
JournalJournal of Global Pharma Technology
Volume9
Issue number3
Publication statusPublished - 01-03-2017

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High Pressure Liquid Chromatography
Artifacts
Names
Methanol
Japan
mecobalamin
AD 20
phosphoric acid

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

@article{3369010a254f42f2a5c1baead058154f,
title = "RP-HPLC method development and validation for the estimation of methylcobalamin in bulk",
abstract = "The subsequent artefact entail of extremely subtle and informal Reversed phase HPLC (RP-HPLC) technique for approximation ofMethylcobalamin in large quantity. Shimadzu LC-20- AD(Kyoto, Japan), with auto sampler and (PDA/UV) detector was used for the separation of Methylcobalamin.C18 Hypersil BDS (5.0 micron,150 x 4.6mm) column was aimed at the separation and mobile phase(MP) comprising of(55:45 v/v) Methanol:o-phosphoric acid 0.02{\%}v/v pH 2.3 was castoffby keeping the rate offlow1 ml/minalong the effluents supervised on 223nm. linearity is undeviating having line equationf(x) =4.87512e-006*x-0.702284 with correlation coefficient(R2) of 0.997. Theprocessremained precise with 0.5{\%} RSD(intra-day precision) & (Inter-day precision) is 0.43{\%}. The LOD & LOQ was0.1499μg/ml and 0.4542μg/ml respectively.The establishedprocess wasvalidated forlinearity,system suitability,precision, and robustness; henceforthverifies in order that this one is exceedinglyrelevantto approximate Methylcobalamin in routine analysis.Methods name: Estimation of Methylcobalaminby HPLC.",
author = "Chamle, {Ashish Hanumantrao} and Shane, {Nazare Lobo John} and Girish Pai and Aravind Pai and Sathyanarayana, {Muddukrishna Badamane}",
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RP-HPLC method development and validation for the estimation of methylcobalamin in bulk. / Chamle, Ashish Hanumantrao; Shane, Nazare Lobo John; Pai, Girish; Pai, Aravind; Sathyanarayana, Muddukrishna Badamane.

In: Journal of Global Pharma Technology, Vol. 9, No. 3, 01.03.2017, p. 6-12.

Research output: Contribution to journalArticle

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T1 - RP-HPLC method development and validation for the estimation of methylcobalamin in bulk

AU - Chamle, Ashish Hanumantrao

AU - Shane, Nazare Lobo John

AU - Pai, Girish

AU - Pai, Aravind

AU - Sathyanarayana, Muddukrishna Badamane

PY - 2017/3/1

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