Salivary protein markers

A noninvasive protein profile-based method for the early diagnosis of oral premalignancy and malignancy

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9 Citations (Scopus)

Abstract

An ultra-sensitive hyphenated technique, high-performance liquid chromatography-laser-induced fluorescence detection protein profiling of saliva, is evaluated for early detection and diagnosis of oral premalignancy and malignancy. Calibration sets of protein profiles of unstimulated whole saliva are collected from clinically/pathologically normal, premalignant, and malignant subjects and used as standards. Three parameters-scores of factors, sum of squared residuals, and Mahalanobis distance-derived from principal component analysis of protein profiles of the standard calibration sets, and blind samples are used for "match/no-match" diagnosis of the blind samples. Analyses of the results show that the method is capable of differentiating normal, premalignant, and malignant conditions with the sensitivity and specificity of 79% and 78%, respectively. The technique provides a fast, highly objective (free from personal judgment and statistically defined), and noninvasive diagnostic method for screening and early detection of oral cancer.

Original languageEnglish
Article number101317
JournalJournal of Biomedical Optics
Volume18
Issue number10
DOIs
Publication statusPublished - 06-11-2013

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Salivary Proteins and Peptides
markers
saliva
proteins
Proteins
profiles
Calibration
liquid chromatography
High performance liquid chromatography
principal components analysis
laser induced fluorescence
Principal component analysis
Screening
screening
cancer
Fluorescence
Lasers
sensitivity

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

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abstract = "An ultra-sensitive hyphenated technique, high-performance liquid chromatography-laser-induced fluorescence detection protein profiling of saliva, is evaluated for early detection and diagnosis of oral premalignancy and malignancy. Calibration sets of protein profiles of unstimulated whole saliva are collected from clinically/pathologically normal, premalignant, and malignant subjects and used as standards. Three parameters-scores of factors, sum of squared residuals, and Mahalanobis distance-derived from principal component analysis of protein profiles of the standard calibration sets, and blind samples are used for {"}match/no-match{"} diagnosis of the blind samples. Analyses of the results show that the method is capable of differentiating normal, premalignant, and malignant conditions with the sensitivity and specificity of 79{\%} and 78{\%}, respectively. The technique provides a fast, highly objective (free from personal judgment and statistically defined), and noninvasive diagnostic method for screening and early detection of oral cancer.",
author = "Ajeetkumar Patil and Choudhari, {Khoobram S.} and Unnikrishnan, {Vayakkara Kolaprath} and Nandita Shenoy and Ravikiran Ongole and Pai, {Keerthilatha M.} and Kartha, {Vasudevan Bhaskaran} and Santhosh Chidangil",
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AU - Patil, Ajeetkumar

AU - Choudhari, Khoobram S.

AU - Unnikrishnan, Vayakkara Kolaprath

AU - Shenoy, Nandita

AU - Ongole, Ravikiran

AU - Pai, Keerthilatha M.

AU - Kartha, Vasudevan Bhaskaran

AU - Chidangil, Santhosh

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