Salt split technique

A useful tool in the diagnosis of subepidermal bullous disorders

Abhishek De, Raghavendra Rao, C. Balachandran

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Direct immunofluorescence (DIF) is the gold standard in the diagnosis of immunobullous diseases. However, it cannot reliably differentiate various subtypes of subepidermal immune- bullous diseases (SIBD). Salt split technique (SST) could be used under such circumstances to differentiate them. There is paucity of reports in the Indian literature regarding the SST. Aim: This study was designed to evaluate the utility of direct SST in subepidermal blistering diseases. Materials and Methods: Fourteen clinically diagnosed cases of subepidermal blistering diseases were included in the study. Two perilesional punch biopsies were taken one each for DIF and salt split study. Results: Linear basement membrane zone band with IgG and/or C3 was seen in 14 cases of patients BP. Salt split study showed epidermal or mixed pattern of deposits in 12 patients and exclusive floor pattern in two patients. The diagnosis was revised in these two patients to epidermolysis bullosa acquisita. Conclusion: SST is a simple, inexpensive procedure and should be routinely employed in the diagnosis of subepidermal bullous diseases.

Original languageEnglish
Pages (from-to)334-336
Number of pages3
JournalIndian Journal of Dermatology
Volume55
Issue number4
DOIs
Publication statusPublished - 01-10-2010

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Salts
Direct Fluorescent Antibody Technique
Epidermolysis Bullosa Acquisita
Immune System Diseases
Basement Membrane
Immunoglobulin G
Biopsy

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

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abstract = "Background: Direct immunofluorescence (DIF) is the gold standard in the diagnosis of immunobullous diseases. However, it cannot reliably differentiate various subtypes of subepidermal immune- bullous diseases (SIBD). Salt split technique (SST) could be used under such circumstances to differentiate them. There is paucity of reports in the Indian literature regarding the SST. Aim: This study was designed to evaluate the utility of direct SST in subepidermal blistering diseases. Materials and Methods: Fourteen clinically diagnosed cases of subepidermal blistering diseases were included in the study. Two perilesional punch biopsies were taken one each for DIF and salt split study. Results: Linear basement membrane zone band with IgG and/or C3 was seen in 14 cases of patients BP. Salt split study showed epidermal or mixed pattern of deposits in 12 patients and exclusive floor pattern in two patients. The diagnosis was revised in these two patients to epidermolysis bullosa acquisita. Conclusion: SST is a simple, inexpensive procedure and should be routinely employed in the diagnosis of subepidermal bullous diseases.",
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Salt split technique : A useful tool in the diagnosis of subepidermal bullous disorders. / De, Abhishek; Rao, Raghavendra; Balachandran, C.

In: Indian Journal of Dermatology, Vol. 55, No. 4, 01.10.2010, p. 334-336.

Research output: Contribution to journalArticle

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AU - Rao, Raghavendra

AU - Balachandran, C.

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