Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter

Prasanna Bhomkar, Chandrama P. Upadhyay, Mukesh Saxena, Annamalai Muthusamy, N. Shiva Prakash, Mikhail Pooggin, Thomas Hohn, Neera B. Sarin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A reproducible and efficient transformation system utilizing the nodal regions of embryonal axis of blackgram (Vigna mungo L. Hepper) has been established via Agrobacterium tumefaciens. This is a report of genetic transformation of Vigna mungo for value addition of an agronomic trait, wherein the gene of interest, the glyoxalase I driven by a novel constitutive Cestrum yellow leaf curling viral promoter has been transferred for alleviating salt stress. The overexpression of this gene under the constitutive CaMV 35S promoter had earlier been shown to impart salt, heavy metal and drought stress tolerance in the model plant, tobacco. Molecular analyses of four independent transgenic lines performed by PCR, Southern and western blot revealed the stable integration of the transgene in the progeny. The transformation frequency was ca. 2.25% and the time required for the generation of transgenic plants was 10-11 weeks. Exposure of T1 transgenic plants as well as untransformed control plants to salt stress (100 mM NaCl) revealed that the transgenic plants survived under salt stress and set seed whereas the untransformed control plants failed to survive. The higher level of Glyoxalase I activity in transgenic lines was directly correlated with their ability to withstand salt stress. To the best of our knowledge this is the only report of engineering abiotic stress tolerance in blackgram.

Original languageEnglish
Pages (from-to)169-181
Number of pages13
JournalMolecular Breeding
Volume22
Issue number2
DOIs
Publication statusPublished - 09-2008

Fingerprint

Cestrum
Lactoylglutathione Lyase
leaf curling
Vigna mungo
black gram
salt stress
Salts
promoter regions
genetically modified organisms
Viruses
Genetically Modified Plants
transgenic plants
viruses
Genes
genes
gene overexpression
genetic transformation
Genetic Transformation
seed set
Agrobacterium radiobacter

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Bhomkar, Prasanna ; Upadhyay, Chandrama P. ; Saxena, Mukesh ; Muthusamy, Annamalai ; Shiva Prakash, N. ; Pooggin, Mikhail ; Hohn, Thomas ; Sarin, Neera B. / Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter. In: Molecular Breeding. 2008 ; Vol. 22, No. 2. pp. 169-181.
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abstract = "A reproducible and efficient transformation system utilizing the nodal regions of embryonal axis of blackgram (Vigna mungo L. Hepper) has been established via Agrobacterium tumefaciens. This is a report of genetic transformation of Vigna mungo for value addition of an agronomic trait, wherein the gene of interest, the glyoxalase I driven by a novel constitutive Cestrum yellow leaf curling viral promoter has been transferred for alleviating salt stress. The overexpression of this gene under the constitutive CaMV 35S promoter had earlier been shown to impart salt, heavy metal and drought stress tolerance in the model plant, tobacco. Molecular analyses of four independent transgenic lines performed by PCR, Southern and western blot revealed the stable integration of the transgene in the progeny. The transformation frequency was ca. 2.25{\%} and the time required for the generation of transgenic plants was 10-11 weeks. Exposure of T1 transgenic plants as well as untransformed control plants to salt stress (100 mM NaCl) revealed that the transgenic plants survived under salt stress and set seed whereas the untransformed control plants failed to survive. The higher level of Glyoxalase I activity in transgenic lines was directly correlated with their ability to withstand salt stress. To the best of our knowledge this is the only report of engineering abiotic stress tolerance in blackgram.",
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Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter. / Bhomkar, Prasanna; Upadhyay, Chandrama P.; Saxena, Mukesh; Muthusamy, Annamalai; Shiva Prakash, N.; Pooggin, Mikhail; Hohn, Thomas; Sarin, Neera B.

In: Molecular Breeding, Vol. 22, No. 2, 09.2008, p. 169-181.

Research output: Contribution to journalArticle

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AU - Hohn, Thomas

AU - Sarin, Neera B.

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