Scavenging of free radicals and total phenols of methanol extract of Azima tetracantha lam

G. Gayathri, Bindu R. Nair, V. Babu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To report the antioxidant activity, phenolic acid profile of the Azima tetracantha methanolic extract and find the correlation between the total phenolic content and their relative antioxidant activity.

Methods: The total antioxidant activity, hydroxyl radical and hydrogen peroxide scavenging of A. tetracantha methanol extract was evaluated by adopting various standard methods. Total phenolic content was estimated from dried methanolic extract and the phenolic acids in the extract were identified by HPLC.

Results: The methanolic extract of A. tetracantha showed free radical scavenging in a concentration dependant manner. The studies showed that the extract possess high free radical scavenging property, especially against hydroxyl radicals (67.61±0.42%). The correlation analysis results showed linear relation between phenolics content of methanolic extract and antioxidant assays (R2=0.97, 0.94, 0.99 and 0.98, significance at P<.0.001). The phenolic acid profile showed the presence of both benzoic acid and cinnamic acid derivatives, well known for their antioxidant potential.

Conclusion: The current observations suggested that the methanol extract of A. tetracantha exhibited multiple antioxidant activities, by scavenging free radicals. So, it can be concluded that the extract is a source of natural antioxidant, suggesting its traditional use as a rejuvenating agent.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume6
Issue number9
Publication statusPublished - 01-01-2014

Fingerprint

Phenols
Free Radicals
Methanol
Antioxidants
Hydroxyl Radical
Benzoic Acid
Hydrogen Peroxide
High Pressure Liquid Chromatography
phenolic acid

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{2bea57e8354240e9b19010eaea7e1349,
title = "Scavenging of free radicals and total phenols of methanol extract of Azima tetracantha lam",
abstract = "Objective: To report the antioxidant activity, phenolic acid profile of the Azima tetracantha methanolic extract and find the correlation between the total phenolic content and their relative antioxidant activity.Methods: The total antioxidant activity, hydroxyl radical and hydrogen peroxide scavenging of A. tetracantha methanol extract was evaluated by adopting various standard methods. Total phenolic content was estimated from dried methanolic extract and the phenolic acids in the extract were identified by HPLC.Results: The methanolic extract of A. tetracantha showed free radical scavenging in a concentration dependant manner. The studies showed that the extract possess high free radical scavenging property, especially against hydroxyl radicals (67.61±0.42{\%}). The correlation analysis results showed linear relation between phenolics content of methanolic extract and antioxidant assays (R2=0.97, 0.94, 0.99 and 0.98, significance at P<.0.001). The phenolic acid profile showed the presence of both benzoic acid and cinnamic acid derivatives, well known for their antioxidant potential.Conclusion: The current observations suggested that the methanol extract of A. tetracantha exhibited multiple antioxidant activities, by scavenging free radicals. So, it can be concluded that the extract is a source of natural antioxidant, suggesting its traditional use as a rejuvenating agent.",
author = "G. Gayathri and Nair, {Bindu R.} and V. Babu",
year = "2014",
month = "1",
day = "1",
language = "English",
volume = "6",
pages = "347--351",
journal = "International Journal of Pharmacy and Pharmaceutical Sciences",
issn = "0975-1491",
publisher = "IJPPS",
number = "9",

}

Scavenging of free radicals and total phenols of methanol extract of Azima tetracantha lam. / Gayathri, G.; Nair, Bindu R.; Babu, V.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, No. 9, 01.01.2014, p. 347-351.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Scavenging of free radicals and total phenols of methanol extract of Azima tetracantha lam

AU - Gayathri, G.

AU - Nair, Bindu R.

AU - Babu, V.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective: To report the antioxidant activity, phenolic acid profile of the Azima tetracantha methanolic extract and find the correlation between the total phenolic content and their relative antioxidant activity.Methods: The total antioxidant activity, hydroxyl radical and hydrogen peroxide scavenging of A. tetracantha methanol extract was evaluated by adopting various standard methods. Total phenolic content was estimated from dried methanolic extract and the phenolic acids in the extract were identified by HPLC.Results: The methanolic extract of A. tetracantha showed free radical scavenging in a concentration dependant manner. The studies showed that the extract possess high free radical scavenging property, especially against hydroxyl radicals (67.61±0.42%). The correlation analysis results showed linear relation between phenolics content of methanolic extract and antioxidant assays (R2=0.97, 0.94, 0.99 and 0.98, significance at P<.0.001). The phenolic acid profile showed the presence of both benzoic acid and cinnamic acid derivatives, well known for their antioxidant potential.Conclusion: The current observations suggested that the methanol extract of A. tetracantha exhibited multiple antioxidant activities, by scavenging free radicals. So, it can be concluded that the extract is a source of natural antioxidant, suggesting its traditional use as a rejuvenating agent.

AB - Objective: To report the antioxidant activity, phenolic acid profile of the Azima tetracantha methanolic extract and find the correlation between the total phenolic content and their relative antioxidant activity.Methods: The total antioxidant activity, hydroxyl radical and hydrogen peroxide scavenging of A. tetracantha methanol extract was evaluated by adopting various standard methods. Total phenolic content was estimated from dried methanolic extract and the phenolic acids in the extract were identified by HPLC.Results: The methanolic extract of A. tetracantha showed free radical scavenging in a concentration dependant manner. The studies showed that the extract possess high free radical scavenging property, especially against hydroxyl radicals (67.61±0.42%). The correlation analysis results showed linear relation between phenolics content of methanolic extract and antioxidant assays (R2=0.97, 0.94, 0.99 and 0.98, significance at P<.0.001). The phenolic acid profile showed the presence of both benzoic acid and cinnamic acid derivatives, well known for their antioxidant potential.Conclusion: The current observations suggested that the methanol extract of A. tetracantha exhibited multiple antioxidant activities, by scavenging free radicals. So, it can be concluded that the extract is a source of natural antioxidant, suggesting its traditional use as a rejuvenating agent.

UR - http://www.scopus.com/inward/record.url?scp=84907810034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907810034&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 347

EP - 351

JO - International Journal of Pharmacy and Pharmaceutical Sciences

JF - International Journal of Pharmacy and Pharmaceutical Sciences

SN - 0975-1491

IS - 9

ER -