"scratch it out": Carbon copy based paper devices for microbial assays and liver disease diagnosis

Amrutha Hasandka, Anusha Prabhu, Akshata Prabhu, Hardik Ramesh Singhal, Giri Nandagopal M. S., Revathi Shenoy, Naresh Kumar Mani

Research output: Contribution to journalArticlepeer-review

Abstract

We present a facile paper-based microfluidic device fabrication technique leveraging off-the-shelf carbon paper for the deposition of hydrophobic barriers using a novel "stencil scratching"method. This exceedingly frugal approach (0.05$) requires practically no technical training to employ. Hydrophobic barriers fabricated using this approach offer a width of 3 mm and a hydrophilic channel width of 849 μm, with an ability to confine major aqueous solvents without leakage. The utility of the device is demonstrated by porting a cell viability assay showing a limit-of-detection (LOD) of 0.6 × 108 CFU mL-1 and bilirubin assay with human serum showing a detection range of 1.76-6.9 mg dL-1 and a limit-of-detection (LOD) of 1.76 mg dL-1. The intuitiveness and economic viability of the fabrication method afford it great potential in the field of point-of-care diagnostics geared towards providing testing infrastructure in resource-scarce regions globally.

Original languageEnglish
Pages (from-to)3172-3180
Number of pages9
JournalAnalytical Methods
Volume13
Issue number28
DOIs
Publication statusPublished - 28-07-2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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