Screening marine-derived endophytic fungi for xylan-degrading enzymes

N. Thirunavukkarasu, Ben Jahnes, Arthur Broadstock, M. B. Govinda Rajulu, T. S. Murali, Venkat Gopalan, T. S. Suryanarayanan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Marine-derived fungi surviving as symptomless endophytes in seaweeds and seagrasses were screened for production of xylan-degrading enzymes. Of the eight endophyte isolates obtained from five different seagrasses and another eight from six different marine algae, half of them exhibited xylanase activity in an agar plate assay. Further examination of these lead candidates using spectrophotometric assays revealed that Trichoderma harzianum, endophytic in the brown alga Sargassum wightii, had the maximum secreted xylanase and xylosidase activity. Moreover, this fungus could grow in NaCl-containing media (up to 1.2 M NaCl), and inclusion of 0.26 M NaCl in the media elicited a two- and three-fold increase in extracellular xylanase and xylosidase activity respectively. These findings highlight the potential of prospecting marinederived fungal endophytes to identify novel cell-wall degrading enzymes of value to the biofuel industry.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalCurrent Science
Volume109
Issue number1
Publication statusPublished - 2015

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xylan
xylanases
endophytes
screening
algae
fungi
Sargassum wightii
enzymes
bioenergy industry
Phaeophyceae
Trichoderma harzianum
assays
macroalgae
agar
cell walls
seagrasses

All Science Journal Classification (ASJC) codes

  • General

Cite this

Thirunavukkarasu, N., Jahnes, B., Broadstock, A., Govinda Rajulu, M. B., Murali, T. S., Gopalan, V., & Suryanarayanan, T. S. (2015). Screening marine-derived endophytic fungi for xylan-degrading enzymes. Current Science, 109(1), 112-120.
Thirunavukkarasu, N. ; Jahnes, Ben ; Broadstock, Arthur ; Govinda Rajulu, M. B. ; Murali, T. S. ; Gopalan, Venkat ; Suryanarayanan, T. S. / Screening marine-derived endophytic fungi for xylan-degrading enzymes. In: Current Science. 2015 ; Vol. 109, No. 1. pp. 112-120.
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Thirunavukkarasu, N, Jahnes, B, Broadstock, A, Govinda Rajulu, MB, Murali, TS, Gopalan, V & Suryanarayanan, TS 2015, 'Screening marine-derived endophytic fungi for xylan-degrading enzymes', Current Science, vol. 109, no. 1, pp. 112-120.

Screening marine-derived endophytic fungi for xylan-degrading enzymes. / Thirunavukkarasu, N.; Jahnes, Ben; Broadstock, Arthur; Govinda Rajulu, M. B.; Murali, T. S.; Gopalan, Venkat; Suryanarayanan, T. S.

In: Current Science, Vol. 109, No. 1, 2015, p. 112-120.

Research output: Contribution to journalArticle

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AU - Jahnes, Ben

AU - Broadstock, Arthur

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Thirunavukkarasu N, Jahnes B, Broadstock A, Govinda Rajulu MB, Murali TS, Gopalan V et al. Screening marine-derived endophytic fungi for xylan-degrading enzymes. Current Science. 2015;109(1):112-120.