Screening of chitin deacetylase producing microbes from marine source using a novel receptor on agar plate

Goutam Mohan Pawaskar, Srikala Pangannaya, Keyur Raval, Darshak R. Trivedi, Ritu Raval

Research output: Contribution to journalArticle

Abstract

Chitosan is a deacetylated form of naturally occurring polymer; chitin. On an industrial scale, the deacetylation of chitin to chitosan is performed using harsh chemicals like sodium hydroxide. This not only adds to the environmental pollution but the product is also random in terms of its deacetylation. This shortcoming can be addressed by using enzymes like chitin deacetylase (CDA). The screening of these organisms would require a reliable, fast and sensitive screening method. The deacetylation of chitin into chitosan, releases acetate as the byproduct of the reaction. A receptor which specifically binds to the acetate ion was synthesized chemically. The receptor upon binding with the acetate ion emitted a fluorescence which could be viewed using the gel documentation unit. The receptor was optimized for the screening of CDA producing microbes with the positive fungal control as Penicillium sp. and bacterial control as Bacillus megaterium. A parallel study with the 4-Nitroacetanilide, the reported screening indicator for CDA was performed. The results obtained with the receptor in the present study were concordant with the 4-Nitroacetanilide. Upon standardization, the protocol was extended for the screening of CDA producing microbes from the marine crustacean dumped soil and water samples. The CDA activity of these microbes was further confirmed using spectrophotometric MBTH assay. This is the first report using this receptor for the screening of CDA producers. The method is not only sensitive but also reproducible and can be extended for a high throughput screening of CDA producers.

Original languageEnglish
Pages (from-to)716-720
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume131
DOIs
Publication statusPublished - 15-06-2019

Fingerprint

chitin deacetylase
Agar
Screening
Chitin
Chitosan
Acetates
Ions
Bacillus megaterium
Sodium Hydroxide
Environmental Pollution
Penicillium
Bacilli
Documentation
Standardization
Byproducts
Assays
Polymers
Pollution
Soil

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Pawaskar, Goutam Mohan ; Pangannaya, Srikala ; Raval, Keyur ; Trivedi, Darshak R. ; Raval, Ritu. / Screening of chitin deacetylase producing microbes from marine source using a novel receptor on agar plate. In: International Journal of Biological Macromolecules. 2019 ; Vol. 131. pp. 716-720.
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Screening of chitin deacetylase producing microbes from marine source using a novel receptor on agar plate. / Pawaskar, Goutam Mohan; Pangannaya, Srikala; Raval, Keyur; Trivedi, Darshak R.; Raval, Ritu.

In: International Journal of Biological Macromolecules, Vol. 131, 15.06.2019, p. 716-720.

Research output: Contribution to journalArticle

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