Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus

Poonam Mishra Chatterjee, Silpi Datta, Deepika Pandey Tiwari, Ritu Raval, Ashok Kumar Dubey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

γ-Polyglutamic acid (γ-PGA) is a biosynthetic outcome of glutamic acid polymerization by microbes. In the current study, we have isolated Bacillus methylotrophicus on solid differential media containing methylene blue. This is the first report mentioning the use of methylene blue to distinguish the monomeric and polymeric form of glutamic acid in the liquid medium using UV-Vis spectrophotometer. Our method can simplify the analytical process of γ-PGA confirmation using the aforementioned studies. This screening protocol is sensitive to the detection of γ-PGA quantities as low as 3 μg/mL; thus, the potent producers can be effectively screened. Furthermore, we have carried out process optimization of the present strain for γ-PGA production wherein we could obtain 1.4-fold improvement in the yield with respect to utilization of carbon source and 2.6-fold increase with respect to nitrogen source under submerged fermentation at a shake flask level. We have shown an increase in γ-PGA titer from 1.5 to 36 g/L using mannitol, monosodium glutamate, peptone, and tween 20.

Original languageEnglish
Pages (from-to)270-288
Number of pages19
JournalApplied Biochemistry and Biotechnology
Volume185
Issue number1
DOIs
Publication statusPublished - 01-05-2018

Fingerprint

Prostaglandins A
Polyglutamic Acid
Biosynthesis
Bacilli
Microorganisms
Fermentation
Bacillus
Acids
Methylene Blue
Ultraviolet spectrophotometers
Glutamic Acid
Sodium Glutamate
Peptones
Polysorbates
Screening
Mannitol
Polymerization
Nitrogen
Carbon
Liquids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

@article{aaba4aa0106949079ae590c95c6e0e89,
title = "Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus",
abstract = "γ-Polyglutamic acid (γ-PGA) is a biosynthetic outcome of glutamic acid polymerization by microbes. In the current study, we have isolated Bacillus methylotrophicus on solid differential media containing methylene blue. This is the first report mentioning the use of methylene blue to distinguish the monomeric and polymeric form of glutamic acid in the liquid medium using UV-Vis spectrophotometer. Our method can simplify the analytical process of γ-PGA confirmation using the aforementioned studies. This screening protocol is sensitive to the detection of γ-PGA quantities as low as 3 μg/mL; thus, the potent producers can be effectively screened. Furthermore, we have carried out process optimization of the present strain for γ-PGA production wherein we could obtain 1.4-fold improvement in the yield with respect to utilization of carbon source and 2.6-fold increase with respect to nitrogen source under submerged fermentation at a shake flask level. We have shown an increase in γ-PGA titer from 1.5 to 36 g/L using mannitol, monosodium glutamate, peptone, and tween 20.",
author = "Chatterjee, {Poonam Mishra} and Silpi Datta and Tiwari, {Deepika Pandey} and Ritu Raval and Dubey, {Ashok Kumar}",
year = "2018",
month = "5",
day = "1",
doi = "10.1007/s12010-017-2654-6",
language = "English",
volume = "185",
pages = "270--288",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "1",

}

Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus. / Chatterjee, Poonam Mishra; Datta, Silpi; Tiwari, Deepika Pandey; Raval, Ritu; Dubey, Ashok Kumar.

In: Applied Biochemistry and Biotechnology, Vol. 185, No. 1, 01.05.2018, p. 270-288.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus

AU - Chatterjee, Poonam Mishra

AU - Datta, Silpi

AU - Tiwari, Deepika Pandey

AU - Raval, Ritu

AU - Dubey, Ashok Kumar

PY - 2018/5/1

Y1 - 2018/5/1

N2 - γ-Polyglutamic acid (γ-PGA) is a biosynthetic outcome of glutamic acid polymerization by microbes. In the current study, we have isolated Bacillus methylotrophicus on solid differential media containing methylene blue. This is the first report mentioning the use of methylene blue to distinguish the monomeric and polymeric form of glutamic acid in the liquid medium using UV-Vis spectrophotometer. Our method can simplify the analytical process of γ-PGA confirmation using the aforementioned studies. This screening protocol is sensitive to the detection of γ-PGA quantities as low as 3 μg/mL; thus, the potent producers can be effectively screened. Furthermore, we have carried out process optimization of the present strain for γ-PGA production wherein we could obtain 1.4-fold improvement in the yield with respect to utilization of carbon source and 2.6-fold increase with respect to nitrogen source under submerged fermentation at a shake flask level. We have shown an increase in γ-PGA titer from 1.5 to 36 g/L using mannitol, monosodium glutamate, peptone, and tween 20.

AB - γ-Polyglutamic acid (γ-PGA) is a biosynthetic outcome of glutamic acid polymerization by microbes. In the current study, we have isolated Bacillus methylotrophicus on solid differential media containing methylene blue. This is the first report mentioning the use of methylene blue to distinguish the monomeric and polymeric form of glutamic acid in the liquid medium using UV-Vis spectrophotometer. Our method can simplify the analytical process of γ-PGA confirmation using the aforementioned studies. This screening protocol is sensitive to the detection of γ-PGA quantities as low as 3 μg/mL; thus, the potent producers can be effectively screened. Furthermore, we have carried out process optimization of the present strain for γ-PGA production wherein we could obtain 1.4-fold improvement in the yield with respect to utilization of carbon source and 2.6-fold increase with respect to nitrogen source under submerged fermentation at a shake flask level. We have shown an increase in γ-PGA titer from 1.5 to 36 g/L using mannitol, monosodium glutamate, peptone, and tween 20.

UR - http://www.scopus.com/inward/record.url?scp=85033561201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033561201&partnerID=8YFLogxK

U2 - 10.1007/s12010-017-2654-6

DO - 10.1007/s12010-017-2654-6

M3 - Article

AN - SCOPUS:85033561201

VL - 185

SP - 270

EP - 288

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1

ER -