Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein

B. Shívananda Nayak, N. Jagadish Ulloor, B. Shívaraj

Research output: Contribution to journalArticle

Abstract

Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatograpbed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent α2-globulins.

Original languageEnglish
Pages (from-to)503-510
Number of pages8
JournalProtein and Peptide Letters
Volume9
Issue number6
DOIs
Publication statusPublished - 01-12-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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