Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein

B. Shívananda Nayak, N. Jagadish Ulloor, B. Shívaraj

Research output: Contribution to journalArticle

Abstract

Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatograpbed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent α2-globulins.

Original languageEnglish
Pages (from-to)503-510
Number of pages8
JournalProtein and Peptide Letters
Volume9
Issue number6
DOIs
Publication statusPublished - 01-12-2002
Externally publishedYes

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Alocasia
Macroglobulins
Haptoglobins
Serum
Plant Proteins
Proteins
Globulins
Ammonium Sulfate
Precipitates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Nayak, B. Shívananda ; Ulloor, N. Jagadish ; Shívaraj, B. / Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein. In: Protein and Peptide Letters. 2002 ; Vol. 9, No. 6. pp. 503-510.
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Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein. / Nayak, B. Shívananda; Ulloor, N. Jagadish; Shívaraj, B.

In: Protein and Peptide Letters, Vol. 9, No. 6, 01.12.2002, p. 503-510.

Research output: Contribution to journalArticle

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