Self-assembling peptide nanostructures on aligned poly(lactide-co-glycolide) nanofibers for the functional regeneration of sciatic nerve

Manasa Nune, Anuradha Subramanian, Uma Maheswari Krishnan, Suraj Sasidhara Kaimal, Swaminathan Sethuraman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: Regeneration of functional peripheral nerve tissue at critical-sized defect requires extracellular matrix analogs impregnated with appropriate biosignals to regulate the cell fate process and subsequent tissue progression. The aim of the study was to develop electrospun aligned nanofibers as architectural analogs integrated with RADA16-I-BMHP1 as biofunctional peptides. Materials & methods: Aligned poly(lactide-co-glycolide) (PLGA)-RADA16-I-BMHP1 nanofibers were fabricated and characterized for their in vitro potential using rat Schwann cell line and in vivo potential using a 10 mm sciatic nerve transection rat model. Results: PLGA-peptide scaffolds significantly promoted higher expression of genotypic markers and bipolar extension of Schwann cells. Further, PLGA-peptide treated animals promoted the native collagen organization, remyelination and showed significantly higher recovery of sensorimotor and motor function than PLGA-treated groups (p < 0.05). Conclusion: Our results demonstrate that self-assembling peptide nanostructures on aligned PLGA nanofibers provided better cell-matrix communication with significant functional restoration of the sciatic nerve.

Original languageEnglish
Pages (from-to)219-235
Number of pages17
JournalNanomedicine
Volume12
Issue number3
DOIs
Publication statusPublished - 01-02-2017

Fingerprint

motor function
Nanofibers
Polyglactin 910
Nanostructures
Sciatic Nerve
peptide
Peptides
restoration
Regeneration
regeneration
animal
organization
communication
Schwann Cells
Rats
Group
Cells
Tissue
matrix
collagen

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Development
  • Materials Science(all)

Cite this

Nune, Manasa ; Subramanian, Anuradha ; Krishnan, Uma Maheswari ; Kaimal, Suraj Sasidhara ; Sethuraman, Swaminathan. / Self-assembling peptide nanostructures on aligned poly(lactide-co-glycolide) nanofibers for the functional regeneration of sciatic nerve. In: Nanomedicine. 2017 ; Vol. 12, No. 3. pp. 219-235.
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Self-assembling peptide nanostructures on aligned poly(lactide-co-glycolide) nanofibers for the functional regeneration of sciatic nerve. / Nune, Manasa; Subramanian, Anuradha; Krishnan, Uma Maheswari; Kaimal, Suraj Sasidhara; Sethuraman, Swaminathan.

In: Nanomedicine, Vol. 12, No. 3, 01.02.2017, p. 219-235.

Research output: Contribution to journalArticle

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AU - Nune, Manasa

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AU - Kaimal, Suraj Sasidhara

AU - Sethuraman, Swaminathan

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